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Xenopus egg extracts treated with the protein kinase inhibitor 6-dimethylaminopurine (6-DMAP) are unable to support the initiation of DNA replication. Nuclei assembled in 6-DMAP extracts behave as though they are in G2, and will not undergo another round of DNA replication until passage through mitosis. 6-DMAP extracts are functionally devoid of a replication factor that modifies chromatin in early G1 before nuclear envelope assembly, but which is itself incapable of crossing the nuclear envelope. This chromatin modification is capable of supporting only a single round of semiconservative replication. The behavior of this replication factor is sufficient to explain why eukaryotic DNA is replicated once and only once in each cell cycle, and conforms to the previous model of a Replication Licensing Factor. Cell cycle analysis shows that this putative Licensing Factor is inactive during metaphase, but becomes rapidly activated on exit from metaphase when it can modify chromatin before nuclear envelope assembly is complete.
Bakken,
Mapping of transcription initiation and termination signals on Xenopus laevis ribosomal DNA.
1982, Pubmed,
Xenbase
Bakken,
Mapping of transcription initiation and termination signals on Xenopus laevis ribosomal DNA.
1982,
Pubmed
,
Xenbase
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Blow,
Replication of purified DNA in Xenopus egg extract is dependent on nuclear assembly.
1990,
Pubmed
,
Xenbase
Blow,
Nuclei act as independent and integrated units of replication in a Xenopus cell-free DNA replication system.
1987,
Pubmed
,
Xenbase
Blow,
A role for the nuclear envelope in controlling DNA replication within the cell cycle.
1988,
Pubmed
,
Xenbase
Blow,
A cdc2-like protein is involved in the initiation of DNA replication in Xenopus egg extracts.
1990,
Pubmed
,
Xenbase
Broek,
Involvement of p34cdc2 in establishing the dependency of S phase on mitosis.
1991,
Pubmed
Coverley,
Reversible effects of nuclear membrane permeabilization on DNA replication: evidence for a positive licensing factor.
1993,
Pubmed
,
Xenbase
Cox,
DNA replication in cell-free extracts from Xenopus eggs is prevented by disrupting nuclear envelope function.
1992,
Pubmed
,
Xenbase
Coxon,
Fission yeast cdc21+ belongs to a family of proteins involved in an early step of chromosome replication.
1992,
Pubmed
,
Xenbase
Crevel,
DNA replication in cell-free extracts from Drosophila melanogaster.
1991,
Pubmed
,
Xenbase
de Roeper,
Chromatin dispersal and DNA synthesis in G1 and G2 HeLa cell nuclei injected into Xenopus eggs.
1977,
Pubmed
,
Xenbase
Fang,
Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes.
1991,
Pubmed
,
Xenbase
Felix,
A post-ribosomal supernatant from activated Xenopus eggs that displays post-translationally regulated oscillation of its cdc2+ mitotic kinase activity.
1989,
Pubmed
,
Xenbase
Finlay,
Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.
1987,
Pubmed
,
Xenbase
Finlay,
Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored.
1990,
Pubmed
,
Xenbase
Handeli,
The ts41 mutation in Chinese hamster cells leads to successive S phases in the absence of intervening G2, M, and G1.
1992,
Pubmed
Harland,
Regulated replication of DNA microinjected into eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Hennessy,
A group of interacting yeast DNA replication genes.
1991,
Pubmed
Hennessy,
Subcellular localization of yeast CDC46 varies with the cell cycle.
1990,
Pubmed
Hutchison,
The control of DNA replication in a cell-free extract that recapitulates a basic cell cycle in vitro.
1988,
Pubmed
,
Xenbase
Leno,
The nuclear membrane prevents replication of human G2 nuclei but not G1 nuclei in Xenopus egg extract.
1992,
Pubmed
,
Xenbase
Leno,
The nuclear membrane determines the timing of DNA replication in Xenopus egg extracts.
1991,
Pubmed
,
Xenbase
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Lohka,
Roles of cytosol and cytoplasmic particles in nuclear envelope assembly and sperm pronuclear formation in cell-free preparations from amphibian eggs.
1984,
Pubmed
,
Xenbase
Lohka,
Effects of Ca2+ ions on the formation of metaphase chromosomes and sperm pronuclei in cell-free preparations from unactivated Rana pipiens eggs.
1984,
Pubmed
,
Xenbase
Luca,
Control of programmed cyclin destruction in a cell-free system.
1989,
Pubmed
Meijer,
Cyclic activation of histone H1 kinase during sea urchin egg mitotic divisions.
1988,
Pubmed
Mills,
Replication occurs at discrete foci spaced throughout nuclei replicating in vitro.
1989,
Pubmed
,
Xenbase
Minshull,
Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
1989,
Pubmed
,
Xenbase
Minshull,
The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.
1990,
Pubmed
,
Xenbase
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Neant,
6-Dimethylaminopurine blocks starfish oocyte maturation by inhibiting a relevant protein kinase activity.
1988,
Pubmed
Newmeyer,
Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation.
1986,
Pubmed
,
Xenbase
Newport,
Nuclear reconstitution in vitro: stages of assembly around protein-free DNA.
1987,
Pubmed
,
Xenbase
Rao,
Mammalian cell fusion: studies on the regulation of DNA synthesis and mitosis.
1970,
Pubmed
Roberts,
Cis-acting negative control of DNA replication in eukaryotic cells.
1988,
Pubmed
Roberts,
Negative control of DNA replication in composite SV40-bovine papilloma virus plasmids.
1986,
Pubmed
Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Strausfeld,
Dephosphorylation and activation of a p34cdc2/cyclin B complex in vitro by human CDC25 protein.
1991,
Pubmed
Thömmes,
Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein.
1992,
Pubmed
Usui,
Uncoupled cell cycle without mitosis induced by a protein kinase inhibitor, K-252a.
1991,
Pubmed
Yan,
Mcm2 and Mcm3, two proteins important for ARS activity, are related in structure and function.
1991,
Pubmed
