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Increased hyaluronate synthesis is required for fibroblast detachment and mitosis.
Brecht M
,
Mayer U
,
Schlosser E
,
Prehm P
.
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Human-embryo fibroblasts were synchronized by means of colchicine and cytochalasin, and the production of hyaluronate was determined by [3H]glucosamine incorporation and ion-exchange chromatography. Cells arrested by colchicine synthesized small amounts of hyaluronate, whereas cells blocked by cytochalasin were stimulated in hyaluronate production. When the colchicine block was released, there was an increased synthesis of hyaluronate, which appeared first in the cellular fraction and was then shed into the culture medium. After release of the cytochalasin block, the hyaluronate production declined to that found with unsynchronized cells. A comparable increase of hyaluronate synthase activity was observed during mitosis. When hyaluronate synthesis was blocked by periodate-oxidized UDP-glucuronic acid, the cells were arrested in mitosis before rounding of cells. These results suggest that hyaluronate synthesis is required for detachment and rounding of cells during mitosis.
Abatangelo,
Cell detachment mediated by hyaluronic acid.
1982, Pubmed
Abatangelo,
Cell detachment mediated by hyaluronic acid.
1982,
Pubmed
Barnhart,
Detachment variants of Chinese hamster cells. Hyaluronic acid as a modulator of cell detachment.
1979,
Pubmed
Carter,
Effects of cytochalasins on mammalian cells.
1967,
Pubmed
Fisher,
The influence of the substratum on mesenchyme spreading in vitro.
1979,
Pubmed
Hatcher,
Relationship between cell surface protease activity and doubling time in various normal and transformed cells.
1976,
Pubmed
Hopwood,
Glycosaminoglycan synthesis by cultured human skin fibroblasts after transformation with simian virus 40.
1977,
Pubmed
Hörmann,
Regulation by heparin and hyaluronic acid of the fibronectin-dependent association of collagen, Type III, with macrophages.
1981,
Pubmed
Hronowski,
The effect of cell density on net rates of glycosaminoglycan synthesis and secretion by cultured rat fibroblasts.
1980,
Pubmed
Knudson,
Interactions between human tumor cells and fibroblasts stimulate hyaluronate synthesis.
1984,
Pubmed
Kraemer,
Elevated cell-surface hyaluronate in substrate-attached cells.
1978,
Pubmed
Kraemer,
Cell-cycle dependent desquamation of heparan sulfate from the cell surface.
1972,
Pubmed
Matuoka,
Growth-coupled changes in glucosaminoglycans (heparan sulfate and hyaluronic acid) in normal and transformed human fibroblasts.
1985,
Pubmed
Okada,
Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles.
1982,
Pubmed
Otto,
Cell cycle dependent rate of labelling of cellular and secreted glycosaminoglycans in mouse embryonic fibroblasts.
1980,
Pubmed
Prehm,
Synthesis of hyaluronate in differentiated teratocarcinoma cells. Characterization of the synthase.
1983,
Pubmed
,
Xenbase
Prehm,
Synthesis of hyaluronate in differentiated teratocarcinoma cells. Mechanism of chain growth.
1983,
Pubmed
Prehm,
Hyaluronate is synthesized at plasma membranes.
1984,
Pubmed
Prehm,
Inhibition of hyaluronate synthesis.
1985,
Pubmed
,
Xenbase
Prehm,
Induction of hyaluronic acid synthesis in teratocarcinoma stem cells by retinoic acid.
1980,
Pubmed
SCOTT,
Studies in histochemistry. I. Determination of nucleic acids in microgram amounts of tissue.
1956,
Pubmed
Stubblefield,
Synchronized mammalian cell cultures. I. Cell replication cycle and macromolecular synthesis following brief colcemid arrest of mitosis.
1967,
Pubmed
Tomida,
Effects of adenosine 3':5'-cyclic monophosphate and serum on synthesis of hyaluronic acid in confluent rat fibroblasts.
1977,
Pubmed
Tomida,
Induction of hyaluronic acid synthetase activity in rat fibroblasts by medium change of confluent cultures.
1975,
Pubmed
Toole,
Hyaluronate and invasiveness of the rabbit V2 carcinoma.
1979,
Pubmed
Toole,
Hyaluronate turnover during chondrogenesis in the developing chick limb and axial skeleton.
1972,
Pubmed