Dimitrova DS , Todorov IT , Melendy T , Gilbert DM .

GM56406 NIGMS NIH HHS , GM57233-01 NIGMS NIH HHS , R01 GM057233 NIGMS NIH HHS , R29 GM056406 NIGMS NIH HHS

	Figure 1. Experimental protocol (see text for details). Representative photographs of the types of replication patterns prevailing in the early (first half, euchromatic) or late (second half, three sequential heterochromatic patterns) stages of the 10–12 h S-phase in CHOC 400 cells are displayed.
	Figure 3. PCNA does not bind nuclear components during G1-phase and associates transiently with chromosomal domains at the time when they replicate. (A) The association of PCNA (red) with the earliest-replicating chromatin regions (CldU-tagged, green) was examined in aliquots of the cells used in the experiment displayed in Fig. 2. (B) Late-replicating genomic regions were pulse-labeled with CldU (green) at 8 h in S-phase in a parallel culture of CHOC 400 cells, mitotic cells were collected few hours later, released in the subsequent cell cycle for the same periods of time and processed in the same way as in Fig. 2.
	Figure 4. RPA, but not Mcm2, is present at PCNA-containing active mammalian replication forks. Aliquots of the cell cultures (synchronized in early or mid/late S-phase) used in Fig. 2 were immunostained with PCNA-specific (red) and, either Mcm2-specific (A, green), or RPA-specific (B, green) antibodies. Shown are single sections obtained by dual-color confocal laser scanning microscopy.
	Figure 5. Variations in the amount of chromatin-bound Mcm2 and PCNA during different stages of the cell cycle in CHOC 400 cells. Synchronized cell populations were resuspended in cytoskeleton buffer (for Triton extractions) or transport buffer (for digitonin extractions) and incubated for 5 min on ice with or without addition of permeabilizing agents as described in Materials and Methods. The cellular or nuclear pellets (P) were separated from the soluble fractions (S) by centrifugation. The proteins from each fraction (only the pellets were analyzed in the case of digitonin extractions) were separated by electrophoresis in 10% (A, B, C-Mcm2, and D) or 18% (C, PCNA) SDS–polyacrylamide gels (amounts corresponding to 2 × 105 cells were loaded in each lane), transferred to nylon membranes and probed with an anti-Mcm2 antibody (A–D) or anti-PCNA antibody (C, PCNA and F). (A) Immunoblotting analysis of chromatin-bound Mcm2 at 20, 40, and 60 min after release of CHOC 400 cells from metaphase block. The percentages of cells in different stages of mitosis, determined microscopically after staining aliquots of the synchronized cells with 0.1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI), are indicated on the right for each time point. (B) Immunoblot analysis of chromatin-bound Mcm2 at different times during interphase. The permeabilization of cells with 70 μg/ml digitonin produces nuclei with intact membranes and can be used as a reference for the total amount of nuclear Mcm2 proteins. The relative amounts of chromatin-bound Mcm2 proteins at each time point analyzed were estimated by comparing serial dilutions of the soluble fractions run in parallel to an aliquot of the Triton-resistant fraction and are indicated on the right. (C) Western blots of total CHOC400 cellular protein extract probed with anti-Mcm2 or anti-PCNA antibodies. Positions of molecular mass standards (indicated in kD) are marked on the left of each blot. (D) Hamster Mcm2, like human BM28/Mcm2 (Todorov et al. 1995), exists as different isoforms. A lighter exposure of immunoblots probed with Mcm2-specific antibodies reveals that Mcm2 proteins can be resolved as a doublet of bands (marked with stars) in the soluble protein fraction. Only a slow moving form is detected in the nuclear pellet fraction. (E) Immunofluorescent analysis of Mcm2 chromatin-binding in Triton-extracted CHOC 400 cells at different stages of mitosis. Asynchronous cells grown on coverslips were extracted with 0.5% Triton in CSK buffer for 2 min on ice, fixed with formaldehyde, and immunostained for Mcm2 as in Fig. 2. DNA was stained with DAPI as in A. The arrowheads point to cells in prophase (i) and (iv), metaphase (ii) and (v), and late telophase (iii) and (vi). The arrows point to a cell in late anaphase/early telophase (iii) and (vi). (F) Immunoblot analysis of chromatin-bound PCNA at different times during interphase. The same protein blots used in B were probed with a PCNA-specific antibody. An increase in the total amount of cellular PCNA at the beginning of S-phase is evident, consistent with previously reported data on human PCNA (Morris and Mathews 1989). The level of chromatin-bound PCNA increased after release into S-phase and maximal amount of insoluble PCNA was detected during early S-phase.
	Figure 6. Hamster RPA does not assemble into detergent-resistant pre-RC foci during early G1-phase and associates transiently with chromosomal domains at the specific time of S-phase when they replicate. The association of RPA (red), with earliest-replicating (CldU, green, A) or late-replicating (IdU, green, B) chromatin regions was examined in aliquots of the cells used in Fig. 2. Immunolabeling was performed as in Fig. 2.
	Figure 8. Hamster RPA and PCNA are assembled into detergent-resistant replicative complexes within less than an hour from the start of S-phase. CHOC 400 cells, synchronized in mitosis, were released for 8 h in the following cell cycle (20% of the cells had entered S-phase at this time). The cells were pulse-labeled with 100 μM CldU for 1 min, extracted with Triton and fixed with formaldehyde as described in Materials and Methods. Aliquots of the cells were stained with anti-CldU antibodies (green) and either anti-RPA antibodies (red, A) or anti-PCNA antibodies (red, B). S-phase cells display yellow coloration resulting from the colocalization of RPA or PCNA (red) with nascent DNA (CldU, green). Arrowheads point to nuclei that exhibit positive staining for RPA or PCNA, but no CldU label. Arrows indicate the positions of G1-phase cells (barely visible as faint shadows).

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