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Caenorhabditis elegans senses gentle touch in the six touch receptor neurons (TRNs) using a mechanotransduction complex that contains the pore-forming degenerin/epithelial sodium channel (DEG/ENaC) proteins MEC-4 and MEC-10. Past work has suggested these proteins interact with the paraoxonase-like MEC-6 and the cholesterol-binding stomatin-like MEC-2 proteins. Using single molecule optical imaging in Xenopus oocytes, we found that MEC-4 forms homotrimers and MEC-4 and MEC-10 form 4:4:10 heterotrimers. MEC-6 and MEC-2 do not associate tightly with these trimers and do not influence trimer stoichiometry, indicating that they are not part of the core channel transduction complex. Consistent with the in vitro data, MEC-10, but not MEC-6, formed puncta in TRN neurites that colocalize with MEC-4 when MEC-4 is overexpressed in the TRNs.
Abuin,
Functional architecture of olfactory ionotropic glutamate receptors.
2011, Pubmed
Abuin,
Functional architecture of olfactory ionotropic glutamate receptors.
2011,
Pubmed
Arnadóttir,
The DEG/ENaC protein MEC-10 regulates the transduction channel complex in Caenorhabditis elegans touch receptor neurons.
2011,
Pubmed
Bartoi,
Acid-sensing ion channel (ASIC) 1a/2a heteromers have a flexible 2:1/1:2 stoichiometry.
2014,
Pubmed
,
Xenbase
Benson,
Heteromultimers of DEG/ENaC subunits form H+-gated channels in mouse sensory neurons.
2002,
Pubmed
Brenner,
The genetics of Caenorhabditis elegans.
1974,
Pubmed
Brown,
MEC-2 and MEC-6 in the Caenorhabditis elegans sensory mechanotransduction complex: auxiliary subunits that enable channel activity.
2008,
Pubmed
,
Xenbase
Brown,
Gain-of-function mutations in the MEC-4 DEG/ENaC sensory mechanotransduction channel alter gating and drug blockade.
2007,
Pubmed
,
Xenbase
Butterworth,
Acute ENaC stimulation by cAMP in a kidney cell line is mediated by exocytic insertion from a recycling channel pool.
2005,
Pubmed
Canessa,
Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits.
1994,
Pubmed
,
Xenbase
Chalfie,
Developmental genetics of the mechanosensory neurons of Caenorhabditis elegans.
1981,
Pubmed
Chalfie,
Neurosensory mechanotransduction.
2009,
Pubmed
Chatzigeorgiou,
Spatial asymmetry in the mechanosensory phenotypes of the C. elegans DEG/ENaC gene mec-10.
2010,
Pubmed
Chelur,
Targeted cell killing by reconstituted caspases.
2007,
Pubmed
Chelur,
The mechanosensory protein MEC-6 is a subunit of the C. elegans touch-cell degenerin channel.
2002,
Pubmed
,
Xenbase
Costes,
Automatic and quantitative measurement of protein-protein colocalization in live cells.
2004,
Pubmed
Cueva,
Nanoscale organization of the MEC-4 DEG/ENaC sensory mechanotransduction channel in Caenorhabditis elegans touch receptor neurons.
2007,
Pubmed
Driscoll,
The mec-4 gene is a member of a family of Caenorhabditis elegans genes that can mutate to induce neuronal degeneration.
1991,
Pubmed
Geffeney,
How we feel: ion channel partnerships that detect mechanical inputs and give rise to touch and pain perception.
2012,
Pubmed
Goodman,
MEC-2 regulates C. elegans DEG/ENaC channels needed for mechanosensation.
2002,
Pubmed
,
Xenbase
Gorczyca,
Identification of Ppk26, a DEG/ENaC Channel Functioning with Ppk1 in a Mutually Dependent Manner to Guide Locomotion Behavior in Drosophila.
2014,
Pubmed
Guo,
The role of PPK26 in Drosophila larval mechanical nociception.
2014,
Pubmed
Hicks,
The evolution of function in strictosidine synthase-like proteins.
2011,
Pubmed
Huang,
A stomatin-like protein necessary for mechanosensation in C. elegans.
1995,
Pubmed
Huang,
Gene interactions affecting mechanosensory transduction in Caenorhabditis elegans.
1994,
Pubmed
Huber,
Podocin and MEC-2 bind cholesterol to regulate the activity of associated ion channels.
2006,
Pubmed
Ivanina,
Phosphorylation by protein kinase A of RCK1 K+ channels expressed in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Jain,
Probing cellular protein complexes using single-molecule pull-down.
2011,
Pubmed
Jasti,
Structure of acid-sensing ion channel 1 at 1.9 A resolution and low pH.
2007,
Pubmed
Kamat,
NRA-2, a nicalin homolog, regulates neuronal death by controlling surface localization of toxic Caenorhabditis elegans DEG/ENaC channels.
2014,
Pubmed
,
Xenbase
Liu,
Contribution of Drosophila DEG/ENaC genes to salt taste.
2003,
Pubmed
Mauthner,
Balboa binds to pickpocket in vivo and is required for mechanical nociception in Drosophila larvae.
2014,
Pubmed
Miller,
Immunofluorescence microscopy.
1995,
Pubmed
O'Hagan,
The MEC-4 DEG/ENaC channel of Caenorhabditis elegans touch receptor neurons transduces mechanical signals.
2005,
Pubmed
Qiu,
SWELL1, a plasma membrane protein, is an essential component of volume-regulated anion channel.
2014,
Pubmed
Ranade,
Piezo2 is the major transducer of mechanical forces for touch sensation in mice.
2014,
Pubmed
Ulbrich,
Rules of engagement for NMDA receptor subunits.
2008,
Pubmed
,
Xenbase
Ulbrich,
Subunit counting in membrane-bound proteins.
2007,
Pubmed
,
Xenbase
Young,
Co-localization of nitric oxide synthase immunoreactivity and NADPH diaphorase staining in neurons of the guinea-pig intestine.
1992,
Pubmed
Zhang,
MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch receptor neurons through its stomatin-like domain.
2004,
Pubmed
,
Xenbase
Zhong,
Pickpocket is a DEG/ENaC protein required for mechanical nociception in Drosophila larvae.
2010,
Pubmed
