Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Promoter control of translation in Xenopus oocytes.
Gunkel N
,
Braddock M
,
Thorburn AM
,
Muckenthaler M
,
Kingsman AJ
,
Kingsman SM
.
???displayArticle.abstract???
The HIV-1 promoter directs the high level production of transcripts in Xenopus oocytes. However, despite being exported to the cytoplasm, the transcripts are not translated [M. Braddock, A. M. Thorburn, A. Chambers, G. D. Elliott, G. J. Anderson, A. J. Kingsman and S. M. Kingsman (1990) Cell, 62, 1123-1133]. We have shown previously that this is a function of promoter sequences and is independent of the TAR RNA element that is normally located at the 5' end of all HIV mRNAs. We now show that a three nucleotide substitution at position -340, upstream of the RNA start site, reverses the translation inhibition. This site coincides with a sequence that can bind the haematopoietic transcription factor GATA. The inhibition of translation can also be reversed by treatment with inhibitors of casein kinase II or by injection into the nucleus of antibodies specific for the FRGY2 family of RNP proteins. We suggest that the -340 site influences the quality of the transcription complex such that transcripts are diverted to a nucleus-dependent translation inhibition pathway.
Adams,
Synthesis of a gene for the HIV transactivator protein TAT by a novel single stranded approach involving in vivo gap repair.
1988, Pubmed
Adams,
Synthesis of a gene for the HIV transactivator protein TAT by a novel single stranded approach involving in vivo gap repair.
1988,
Pubmed
Barker,
Nonsense codons within the Rous sarcoma virus gag gene decrease the stability of unspliced viral RNA.
1991,
Pubmed
Blobel,
Gene gating: a hypothesis.
1985,
Pubmed
Bouvet,
A role for transcription and FRGY2 in masking maternal mRNA within Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Braddock,
Blocking of Tat-dependent HIV-1 RNA modification by an inhibitor of RNA polymerase II processivity.
1991,
Pubmed
,
Xenbase
Braddock,
Intron-less RNA injected into the nucleus of Xenopus oocytes accesses a regulated translation control pathway.
1994,
Pubmed
,
Xenbase
Braddock,
A nuclear translational block imposed by the HIV-1 U3 region is relieved by the Tat-TAR interaction.
1990,
Pubmed
,
Xenbase
Braddock,
HIV-1 TAT "activates" presynthesized RNA in the nucleus.
1989,
Pubmed
,
Xenbase
Brinster,
Introns increase transcriptional efficiency in transgenic mice.
1988,
Pubmed
Buchman,
Comparison of intron-dependent and intron-independent gene expression.
1988,
Pubmed
Capco,
Localized protein synthesis during oogenesis of Xenopus laevis: analysis by in situ translation.
1982,
Pubmed
,
Xenbase
Cooney,
Chicken ovalbumin upstream promoter transcription factor binds to a negative regulatory region in the human immunodeficiency virus type 1 long terminal repeat.
1991,
Pubmed
Cummings,
Protein kinase activity associated with stored messenger ribonucleoprotein particles of Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Cummings,
Multiple modifications in the phosphoproteins bound to stored messenger RNA in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Darnbrough,
Identification in Xenopus laevis of a class of oocyte-specific proteins bound to messenger RNA.
1981,
Pubmed
,
Xenbase
Dasgupta,
Myb protein binds to human immunodeficiency virus 1 long terminal repeat (LTR) sequences and transactivates LTR-mediated transcription.
1990,
Pubmed
Dearsly,
Identification of a 60-kDa phosphoprotein that binds stored messenger RNA of Xenopus oocytes.
1985,
Pubmed
,
Xenbase
de la Peña,
Enhancement of mRNA nuclear transport by promoter elements.
1987,
Pubmed
,
Xenbase
Deschamps,
Purification of two thermostable components of messenger ribonucleoprotein particles (mRNPs) from Xenopus laevis oocytes, belonging to a novel class of RNA-binding proteins.
1991,
Pubmed
,
Xenbase
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Du,
Human transcription factor USF stimulates transcription through the initiator elements of the HIV-1 and the Ad-ML promoters.
1993,
Pubmed
Engel,
cis and trans regulation of tissue-specific transcription.
1992,
Pubmed
Enssle,
Determination of mRNA fate by different RNA polymerase II promoters.
1993,
Pubmed
Franza,
The Fos complex and Fos-related antigens recognize sequence elements that contain AP-1 binding sites.
1988,
Pubmed
Garcia,
Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus.
1987,
Pubmed
Giacca,
A human binding site for transcription factor USF/MLTF mimics the negative regulatory element of human immunodeficiency virus type 1.
1992,
Pubmed
Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Guy,
DNA binding factors that bind to the negative regulatory element of the human immunodeficiency virus-1: regulation by nef.
1990,
Pubmed
Jarman,
Characterization of the major regulatory element upstream of the human alpha-globin gene cluster.
1991,
Pubmed
Johnston,
Autoradiography using storage phosphor technology.
1990,
Pubmed
Kick,
Phosphorylation of a 60 kDa polypeptide from Xenopus oocytes blocks messenger RNA translation.
1987,
Pubmed
,
Xenbase
Li,
An in vitro transcription analysis of early responses of the human immunodeficiency virus type 1 long terminal repeat to different transcriptional activators.
1991,
Pubmed
Lu,
Identification of cis-acting repressive sequences within the negative regulatory element of human immunodeficiency virus type 1.
1990,
Pubmed
Marello,
Binding of Xenopus oocyte masking proteins to mRNA sequences.
1992,
Pubmed
,
Xenbase
Nakanishi,
A novel cis-acting element that controls transcription of human immunodeficiency virus type 1 DNA, depending on cell type.
1991,
Pubmed
Neuberger,
The intron requirement for immunoglobulin gene expression is dependent upon the promoter.
1988,
Pubmed
Orchard,
Characterization of a novel T lymphocyte protein which binds to a site related to steroid/thyroid hormone receptor response elements in the negative regulatory sequence of the human immunodeficiency virus long terminal repeat.
1992,
Pubmed
Orchard,
A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat.
1990,
Pubmed
Ratner,
Complete nucleotide sequence of the AIDS virus, HTLV-III.
,
Pubmed
Rhoads,
Mechanism of action and regulation of protein synthesis initiation factor 4E: effects on mRNA discrimination, cellular growth rate, and oncogenesis.
1993,
Pubmed
Richter,
Reversible inhibition of translation by Xenopus oocyte-specific proteins.
,
Pubmed
,
Xenbase
Richter,
Translational control during early development.
1991,
Pubmed
,
Xenbase
Rosen,
The location of cis-acting regulatory sequences in the human T cell lymphotropic virus type III (HTLV-III/LAV) long terminal repeat.
1985,
Pubmed
Saiki,
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
1988,
Pubmed
Siekevitz,
Activation of the HIV-1 LTR by T cell mitogens and the trans-activator protein of HTLV-I.
1987,
Pubmed
Smith,
The same 50-kDa cellular protein binds to the negative regulatory elements of the interleukin 2 receptor alpha-chain gene and the human immunodeficiency virus type 1 long terminal repeat.
1989,
Pubmed
Sommerville,
RNA-binding proteins: masking proteins revealed.
1992,
Pubmed
,
Xenbase
Sommerville,
Histone H4 acetylation and transcription in amphibian chromatin.
1993,
Pubmed
,
Xenbase
Sommerville,
RNA-binding phosphoproteins and the regulation of maternal mRNA in Xenopus.
1990,
Pubmed
,
Xenbase
Sowden,
Multiple cooperative interactions constrain BPV-1 E2 dependent activation of transcription.
1989,
Pubmed
Urlaub,
Nonsense mutations in the dihydrofolate reductase gene affect RNA processing.
1989,
Pubmed
Watanabe,
Specific proteolysis of the c-mos proto-oncogene product by calpain on fertilization of Xenopus eggs.
1989,
Pubmed
,
Xenbase
Watt,
A factor binding GATAAG confers tissue specificity on the promoter of the human zeta-globin gene.
1990,
Pubmed
Yang,
Distinct roles for the two cGATA-1 finger domains.
1992,
Pubmed
Zon,
Expression of GATA-binding proteins during embryonic development in Xenopus laevis.
1991,
Pubmed
,
Xenbase
