Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Neuron
2009 Nov 12;643:355-366. doi: 10.1016/j.neuron.2009.09.018.
Show Gene links
Show Anatomy links
Extracellular Engrailed participates in the topographic guidance of retinal axons in vivo.
Wizenmann A
,
Brunet I
,
Lam J
,
Sonnier L
,
Beurdeley M
,
Zarbalis K
,
Weisenhorn-Vogt D
,
Weinl C
,
Dwivedy A
,
Joliot A
,
Wurst W
,
Holt C
,
Prochiantz A
.
???displayArticle.abstract???
Engrailed transcription factors regulate the expression of guidance cues that pattern retinal axon terminals in the dorsal midbrain. They also act directly to guide axon growth in vitro. We show here that an extracellular En gradient exists in the tectum along the anterior-posterior axis. Neutralizing extracellular Engrailed in vivo with antibodies expressed in the tectum causes temporal axons to map aberrantly to the posterior tectum in chick and Xenopus. Furthermore, posterior membranes from wild-type tecta incubated with anti-Engrailed antibodies or posterior membranes from Engrailed-1 knockout mice exhibit diminished repulsive activity for temporal axons. Since EphrinAs play a major role in anterior-posterior mapping, we tested whether Engrailed cooperates with EphrinA5 in vitro. We find that Engrailed restores full repulsion to axons given subthreshold doses of EphrinA5. Collectively, our results indicate that extracellular Engrailed contributes to retinotectal mapping in vivo by modulating the sensitivity of growth cones to EphrinA.
Auerbach,
A simple procedure for the long-term cultivation of chicken embryos.
1974, Pubmed
Auerbach,
A simple procedure for the long-term cultivation of chicken embryos.
1974,
Pubmed
Auf der Maur,
Antigen-independent selection of intracellular stable antibody frameworks.
2004,
Pubmed
Bovolenta,
Morphogen signaling at the vertebrate growth cone: a few cases or a general strategy?
2005,
Pubmed
Brunet,
The topological role of homeoproteins in the developing central nervous system.
2007,
Pubmed
Brunet,
The transcription factor Engrailed-2 guides retinal axons.
2005,
Pubmed
,
Xenbase
Buhusi,
L1 interaction with ankyrin regulates mediolateral topography in the retinocollicular projection.
2008,
Pubmed
Cheng,
Complementary gradients in expression and binding of ELF-1 and Mek4 in development of the topographic retinotectal projection map.
1995,
Pubmed
Chien,
Navigational errors made by growth cones without filopodia in the embryonic Xenopus brain.
1993,
Pubmed
,
Xenbase
Ciossek,
Eph receptor-ligand interactions are necessary for guidance of retinal ganglion cell axons in vitro.
1998,
Pubmed
Claudepierre,
Implication of neuropilin 2/semaphorin 3F in retinocollicular map formation.
2008,
Pubmed
Di Nardo,
Dendritic localization and activity-dependent translation of Engrailed1 transcription factor.
2007,
Pubmed
Drescher,
In vitro guidance of retinal ganglion cell axons by RAGS, a 25 kDa tectal protein related to ligands for Eph receptor tyrosine kinases.
1995,
Pubmed
Dütting,
Transplantations of the chick eye anlage reveal an early determination of nasotemporal polarity.
1995,
Pubmed
Falk,
Electroporation of cDNA/Morpholinos to targeted areas of embryonic CNS in Xenopus.
2007,
Pubmed
,
Xenbase
Feldheim,
Loss-of-function analysis of EphA receptors in retinotectal mapping.
2004,
Pubmed
Feldheim,
Genetic analysis of ephrin-A2 and ephrin-A5 shows their requirement in multiple aspects of retinocollicular mapping.
2000,
Pubmed
Flanagan,
Neural map specification by gradients.
2006,
Pubmed
Friedman,
Retroviral misexpression of engrailed genes in the chick optic tectum perturbs the topographic targeting of retinal axons.
1996,
Pubmed
Frisén,
Ephrin-A5 (AL-1/RAGS) is essential for proper retinal axon guidance and topographic mapping in the mammalian visual system.
1998,
Pubmed
Furrer,
Slit and Robo control the development of dendrites in Drosophila CNS.
2007,
Pubmed
Gingras,
eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation.
1999,
Pubmed
Haas,
Single-cell electroporation for gene transfer in vivo.
2001,
Pubmed
,
Xenbase
Hanks,
Rescue of the En-1 mutant phenotype by replacement of En-1 with En-2.
1995,
Pubmed
Hansen,
Retinal axon response to ephrin-as shows a graded, concentration-dependent transition from growth promotion to inhibition.
2004,
Pubmed
Hemmati-Brivanlou,
Cephalic expression and molecular characterization of Xenopus En-2.
1991,
Pubmed
,
Xenbase
Holcman,
Modeling homeoprotein intercellular transfer unveils a parsimonious mechanism for gradient and boundary formation in early brain development.
2007,
Pubmed
Hornberger,
Modulation of EphA receptor function by coexpressed ephrinA ligands on retinal ganglion cell axons.
1999,
Pubmed
Itasaki,
A role for gradient en expression in positional specification on the optic tectum.
1996,
Pubmed
Joliot,
Association of Engrailed homeoproteins with vesicles presenting caveolae-like properties.
1997,
Pubmed
Joliot,
Transduction peptides: from technology to physiology.
2004,
Pubmed
Knöll,
Stripe assay to examine axonal guidance and cell migration.
2007,
Pubmed
Lesaffre,
Direct non-cell autonomous Pax6 activity regulates eye development in the zebrafish.
2007,
Pubmed
Logan,
Rostral optic tectum acquires caudal characteristics following ectopic engrailed expression.
1996,
Pubmed
Lohof,
Asymmetric modulation of cytosolic cAMP activity induces growth cone turning.
1992,
Pubmed
,
Xenbase
Mann,
Topographic mapping in dorsoventral axis of the Xenopus retinotectal system depends on signaling through ephrin-B ligands.
2002,
Pubmed
,
Xenbase
McFarlane,
Inhibition of FGF receptor activity in retinal ganglion cell axons causes errors in target recognition.
1996,
Pubmed
,
Xenbase
McLaughlin,
Molecular gradients and development of retinotopic maps.
2005,
Pubmed
Mueller,
Partial respecification of nasotemporal polarity in double-temporal chick and chimeric chick-quail eyes.
1998,
Pubmed
Nakamura,
The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner.
1997,
Pubmed
O'Leary,
Patterning centers, regulatory genes and extrinsic mechanisms controlling arealization of the neocortex.
2002,
Pubmed
O'Leary,
Mechanisms of retinotopic map development: Ephs, ephrins, and spontaneous correlated retinal activity.
2005,
Pubmed
Pfeiffenberger,
Ephrin-As and patterned retinal activity act together in the development of topographic maps in the primary visual system.
2006,
Pubmed
Raper,
The enrichment of a neuronal growth cone collapsing activity from embryonic chick brain.
1990,
Pubmed
Sakaguchi,
Growth cone interactions with a glial cell line from embryonic Xenopus retina.
1989,
Pubmed
,
Xenbase
Schmid,
L1 and NCAM adhesion molecules as signaling coreceptors in neuronal migration and process outgrowth.
2008,
Pubmed
Shigetani,
En-2 regulates the expression of the ligands for Eph type tyrosine kinases in chick embryonic tectum.
1997,
Pubmed
Sonnier,
Progressive loss of dopaminergic neurons in the ventral midbrain of adult mice heterozygote for Engrailed1.
2007,
Pubmed
SPERRY,
CHEMOAFFINITY IN THE ORDERLY GROWTH OF NERVE FIBER PATTERNS AND CONNECTIONS.
1963,
Pubmed
Strochlic,
A role for S1P signalling in axon guidance in the Xenopus visual system.
2008,
Pubmed
,
Xenbase
Strube,
Enhanced intracellular stability of sFv-Fc fusion intrabodies.
2004,
Pubmed
Sugiyama,
Experience-dependent transfer of Otx2 homeoprotein into the visual cortex activates postnatal plasticity.
2008,
Pubmed
Swartz,
Sparking new frontiers: using in vivo electroporation for genetic manipulations.
2001,
Pubmed
,
Xenbase
Thanos,
Specific transcellular carbocyanine-labelling of rat retinal microglia during injury-induced neuronal degeneration.
1991,
Pubmed
Vielmetter,
In vitro assay to test differential substrate affinities of growing axons and migratory cells.
1990,
Pubmed
Walter,
Recognition of position-specific properties of tectal cell membranes by retinal axons in vitro.
1987,
Pubmed
Walter,
Avoidance of posterior tectal membranes by temporal retinal axons.
1987,
Pubmed
Weinl,
Responses of temporal retinal growth cones to ephrinA5-coated beads.
2005,
Pubmed
Wilkinson,
Detection of messenger RNA by in situ hybridization to tissue sections and whole mounts.
1993,
Pubmed
Wurst,
Multiple developmental defects in Engrailed-1 mutant mice: an early mid-hindbrain deletion and patterning defects in forelimbs and sternum.
1994,
Pubmed
Yates,
Topographic-specific axon branching controlled by ephrin-As is the critical event in retinotectal map development.
2001,
Pubmed
