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Localized RNAs are found in a variety of somatic and developing cell types. In many cases, microtubules have been implicated as playing a role in facilitating transport of these RNAs. Here we report that Vg1 RNA, which is localized to the vegetal cortex of Xenopus laevis oocytes, is associated with microtubules in vivo. Because of the ubiquitous nature of tubulin, the association of specific RNAs with microtubules is likely to involve factors that recognize both RNA and microtubules. Vg1 RNA binding protein (Vg1 RBP), previously shown to bind with high affinity to the vegetal localization site in Vg1 RNA, appears to function in this capacity. Vg1 RBP is associated with microtubules: it is enriched in microtubule extracts of oocytes and is also co-precipitated by heterologous, polymerized tubulin. Furthermore, Vg1 RBP binding activity is required for the specific association of Vg1 RNA to microtubules in vitro. These data suggest a general model for how specific RNAs can be localized to particular sites via common cytoskeletal elements.
Ainger,
Transport and localization of exogenous myelin basic protein mRNA microinjected into oligodendrocytes.
1993, Pubmed
Ainger,
Transport and localization of exogenous myelin basic protein mRNA microinjected into oligodendrocytes.
1993,
Pubmed
Bassell,
Association of poly(A) mRNA with microtubules in cultured neurons.
1994,
Pubmed
Clark,
Transient posterior localization of a kinesin fusion protein reflects anteroposterior polarity of the Drosophila oocyte.
1994,
Pubmed
Darnbrough,
Identification in Xenopus laevis of a class of oocyte-specific proteins bound to messenger RNA.
1981,
Pubmed
,
Xenbase
DeSimone,
Identification and characterization of alternatively spliced fibronectin mRNAs expressed in early Xenopus embryos.
1992,
Pubmed
,
Xenbase
Ding,
Localized RNAs and their functions.
1993,
Pubmed
,
Xenbase
Durso,
A calmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1 alpha.
1994,
Pubmed
Edson,
Actin depolymerisation induces process formation on MAP2-transfected non-neuronal cells.
1993,
Pubmed
Elinson,
Changes in levels of polymeric tubulin associated with activation and dorsoventral polarization of the frog egg.
1985,
Pubmed
,
Xenbase
Gard,
Organization, nucleation, and acetylation of microtubules in Xenopus laevis oocytes: a study by confocal immunofluorescence microscopy.
1991,
Pubmed
,
Xenbase
Gard,
Gamma-tubulin is asymmetrically distributed in the cortex of Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Garner,
Selective localization of messenger RNA for cytoskeletal protein MAP2 in dendrites.
1988,
Pubmed
Hirokawa,
Microtubule organization and dynamics dependent on microtubule-associated proteins.
1994,
Pubmed
Matus,
Microtubule-associated proteins and neuronal morphogenesis.
1991,
Pubmed
Melton,
Translocation of a localized maternal mRNA to the vegetal pole of Xenopus oocytes.
,
Pubmed
,
Xenbase
Mowry,
Vegetal messenger RNA localization directed by a 340-nt RNA sequence element in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Murray,
Sequence analysis of cytoplasmic mRNA-binding proteins of Xenopus oocytes identifies a family of RNA-binding proteins.
1992,
Pubmed
,
Xenbase
Oberman,
Two protocols for nonradioactive in situ hybridization to Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Pokrywka,
Microtubules mediate the localization of bicoid RNA during Drosophila oogenesis.
1991,
Pubmed
Pondel,
Localized maternal mRNA related to transforming growth factor beta mRNA is concentrated in a cytokeratin-enriched fraction from Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Rebagliati,
Identification and cloning of localized maternal RNAs from Xenopus eggs.
1985,
Pubmed
,
Xenbase
Schwartz,
A 69-kDa RNA-binding protein from Xenopus oocytes recognizes a common motif in two vegetally localized maternal mRNAs.
1992,
Pubmed
,
Xenbase
Sloboda,
Purification and assay of microtubule-associated proteins (MAPs).
1982,
Pubmed
Theurkauf,
Reorganization of the cytoskeleton during Drosophila oogenesis: implications for axis specification and intercellular transport.
1992,
Pubmed
Theurkauf,
A central role for microtubules in the differentiation of Drosophila oocytes.
1993,
Pubmed
Tonissen,
Analysis of a variant Max sequence expressed in Xenopus laevis.
1994,
Pubmed
,
Xenbase
Vallee,
Purification of microtubules and microtubule-associated proteins from sea urchin eggs and cultured mammalian cells using taxol, and use of exogenous taxol-stabilized brain microtubules for purifying microtubule-associated proteins.
1986,
Pubmed
Vallee,
Reversible assembly purification of microtubules without assembly-promoting agents and further purification of tubulin, microtubule-associated proteins, and MAP fragments.
1986,
Pubmed
Vallee,
Purification of brain microtubules and microtubule-associated protein 1 using taxol.
1986,
Pubmed
Wang,
Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis.
1994,
Pubmed
Weeks,
A maternal mRNA localized to the vegetal hemisphere in Xenopus eggs codes for a growth factor related to TGF-beta.
1987,
Pubmed
,
Xenbase
Wilhelm,
RNA on the move: the mRNA localization pathway.
1993,
Pubmed
Wolffe,
The Y-box factors: a family of nucleic acid binding proteins conserved from Escherichia coli to man.
1992,
Pubmed
Yisraeli,
The material mRNA Vg1 is correctly localized following injection into Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Yisraeli,
A two-step model for the localization of maternal mRNA in Xenopus oocytes: involvement of microtubules and microfilaments in the translocation and anchoring of Vg1 mRNA.
1990,
Pubmed
,
Xenbase
