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XB-ART-37150
Curr Protoc Cell Biol 2001 May 01;Chapter 11:Unit 11.10. doi: 10.1002/0471143030.cb1110s09.
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Preparation and use of interphase Xenopus egg extracts.

Powers M , Evans EK , Yang J , Kornbluth S .


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In this unit, Xenopus eggs are isolated from hormonally primed female frogs, and then the extract is treated with cyclohexamide so it remains in interphase of the cell cycle. In the presence of sperm chromatin and ATP, membrane vesicles in the extract fuse to assemble nuclei, making the extract suitable for studies of DNA replication and nuclear transport.

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Species referenced: Xenopus
Genes referenced: fubp1