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Biophys J
2004 Aug 01;872:873-82. doi: 10.1529/biophysj.104.040550.
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Effects of Kv1.2 intracellular regions on activation of Kv2.1 channels.
Scholle A
,
Zimmer T
,
Koopmann R
,
Engeland B
,
Pongs O
,
Benndorf K
.
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Depolarizing voltage steps activate voltage-dependent K(+) (Kv) channels by moving the voltage sensor, which triggers a coupling reaction leading to the opening of the pore. We constructed chimeric channels in which intracellular regions of slowly activating Kv2.1 channels were replaced by respective regions of rapidly activating Kv1.2 channels. Substitution of either the N-terminus, S4-S5 linker, or C-terminus generated chimeric Kv2.1/1.2 channels with a paradoxically slow and approximately exponential activation time course consisting of a fast and a slow component. Using combined chimeras, each of these Kv1.2 regions further slowed activation at the voltage of 0 mV, irrespective of the nature of the other two regions, whereas at the voltage of 40 mV both slowing and accelerating effects were observed. These results suggest voltage-dependent interactions of the three intracellular regions. This observation was quantified by double-mutant cycle analysis. It is concluded that interactions between N-terminus, S4-S5 linker, and/or C-terminus modulate the activation time course of Kv2.1 channels and that part of these interactions is voltage dependent.
Cha,
Characterizing voltage-dependent conformational changes in the Shaker K+ channel with fluorescence.
1997, Pubmed,
Xenbase
Cha,
Characterizing voltage-dependent conformational changes in the Shaker K+ channel with fluorescence.
1997,
Pubmed
,
Xenbase
Chiara,
A small domain in the N terminus of the regulatory alpha-subunit Kv2. 3 modulates Kv2.1 potassium channel gating.
1999,
Pubmed
Cushman,
Voltage dependent activation of potassium channels is coupled to T1 domain structure.
2000,
Pubmed
del Camino,
Tight steric closure at the intracellular activation gate of a voltage-gated K(+) channel.
2001,
Pubmed
Heginbotham,
The aromatic binding site for tetraethylammonium ion on potassium channels.
1992,
Pubmed
Ho,
Site-directed mutagenesis by overlap extension using the polymerase chain reaction.
1989,
Pubmed
Horovitz,
Double-mutant cycles: a powerful tool for analyzing protein structure and function.
1996,
Pubmed
Jiang,
The principle of gating charge movement in a voltage-dependent K+ channel.
2003,
Pubmed
Jiang,
X-ray structure of a voltage-dependent K+ channel.
2003,
Pubmed
Ju,
The Roles of N- and C-terminal determinants in the activation of the Kv2.1 potassium channel.
2003,
Pubmed
,
Xenbase
Larsson,
Transmembrane movement of the shaker K+ channel S4.
1996,
Pubmed
,
Xenbase
Li,
Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel.
1992,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Liman,
Voltage-sensing residues in the S4 region of a mammalian K+ channel.
1991,
Pubmed
,
Xenbase
Li-Smerin,
A localized interaction surface for voltage-sensing domains on the pore domain of a K+ channel.
2000,
Pubmed
,
Xenbase
Logothetis,
Incremental reductions of positive charge within the S4 region of a voltage-gated K+ channel result in corresponding decreases in gating charge.
1992,
Pubmed
,
Xenbase
Lu,
Coupling between voltage sensors and activation gate in voltage-gated K+ channels.
2002,
Pubmed
,
Xenbase
MacKinnon,
Determination of the subunit stoichiometry of a voltage-activated potassium channel.
1991,
Pubmed
,
Xenbase
Mathur,
Role of the S3-S4 linker in Shaker potassium channel activation.
1997,
Pubmed
,
Xenbase
McCormack,
A role for hydrophobic residues in the voltage-dependent gating of Shaker K+ channels.
1991,
Pubmed
,
Xenbase
Mildvan,
Quantitative interpretations of double mutations of enzymes.
1992,
Pubmed
Milligan,
Local movement in the S2 region of the voltage-gated potassium channel hKv2.1 studied using cysteine mutagenesis.
2000,
Pubmed
,
Xenbase
Minor,
The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel.
2000,
Pubmed
,
Xenbase
Papazian,
Electrostatic interactions of S4 voltage sensor in Shaker K+ channel.
1995,
Pubmed
,
Xenbase
Papazian,
Alteration of voltage-dependence of Shaker potassium channel by mutations in the S4 sequence.
1991,
Pubmed
,
Xenbase
Perozo,
S4 mutations alter gating currents of Shaker K channels.
1994,
Pubmed
,
Xenbase
Planells-Cases,
Mutation of conserved negatively charged residues in the S2 and S3 transmembrane segments of a mammalian K+ channel selectively modulates channel gating.
1995,
Pubmed
,
Xenbase
Scholle,
Structural elements determining activation kinetics in Kv2.1.
2000,
Pubmed
,
Xenbase
Schoppa,
Activation of Shaker potassium channels. III. An activation gating model for wild-type and V2 mutant channels.
1998,
Pubmed
,
Xenbase
Schulteis,
Intersubunit interaction between amino- and carboxyl-terminal cysteine residues in tetrameric shaker K+ channels.
1996,
Pubmed
,
Xenbase
Seoh,
Voltage-sensing residues in the S2 and S4 segments of the Shaker K+ channel.
1996,
Pubmed
,
Xenbase
Sheng,
Presynaptic A-current based on heteromultimeric K+ channels detected in vivo.
1993,
Pubmed
Shieh,
Role of transmembrane segment S5 on gating of voltage-dependent K+ channels.
1997,
Pubmed
,
Xenbase
Starace,
Voltage-dependent proton transport by the voltage sensor of the Shaker K+ channel.
1997,
Pubmed
Tiwari-Woodruff,
Electrostatic interactions between transmembrane segments mediate folding of Shaker K+ channel subunits.
1997,
Pubmed
,
Xenbase
VanDongen,
Alteration and restoration of K+ channel function by deletions at the N- and C-termini.
1990,
Pubmed
Wells,
Additivity of mutational effects in proteins.
1990,
Pubmed
Wilson,
Modulation by protein kinase A of a cloned rat brain potassium channel expressed in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Yellen,
The voltage-gated potassium channels and their relatives.
2002,
Pubmed
Yifrach,
Energetics of pore opening in a voltage-gated K(+) channel.
2002,
Pubmed
,
Xenbase
Yusaf,
Measurement of the movement of the S4 segment during the activation of a voltage-gated potassium channel.
1996,
Pubmed
,
Xenbase
Zagotta,
Shaker potassium channel gating. III: Evaluation of kinetic models for activation.
1994,
Pubmed
,
Xenbase
Zagotta,
Shaker potassium channel gating. II: Transitions in the activation pathway.
1994,
Pubmed
,
Xenbase
