Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1997 Mar 18;946:2215-20. doi: 10.1073/pnas.94.6.2215.
Show Gene links
Show Anatomy links
DNA damage can alter the stability of nucleosomes: effects are dependent on damage type.
Mann DB
,
Springer DL
,
Smerdon MJ
.
???displayArticle.abstract???
We have investigated the effects of DNA damage by (+/-)-anti-benzo[a]pyrene diol epoxide (BPDE) and UV light on the formation of a positioned nucleosome in the Xenopus borealis 5S rRNA gene. Gel-shift analysis of the reconstituted products indicates that BPDE damage facilitates the formation of a nucleosome onto this sequence. Competitive reconstitution experiments show that average levels of 0.5, 0.9, and 2.1 BPDE adducts/146 bp of 5S DNA (i.e., the size of DNA associated with a nucleosome core particle) yield changes of -220, -290, and -540 cal/mol, respectively, in the free energy (delta G) of nucleosome formation. These values yield increases of core histone binding to 5S DNA (K(a)) of 1.4-, 1.6-, and 2.5-fold, compared with undamaged DNA. Conversely, irradiation with UV light decreases nucleosome formation. Irradiation at either 500 or 2500 J/m2 of UV light [0.6 and 0.8 cyclobutane pyrimidine dimer/146 bp (on average), respectively] results in respective changes of +130 and +250 cal/mol. This translates to decreases in core histone binding to irradiated 5S DNA (K(a)) of 1.2- and 1.5-fold compared with undamaged DNA. These results indicate that nucleosome stability can be markedly affected by the formation of certain DNA lesions. Such changes could have major effects on the kinetics of DNA processing events.
Archer,
Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter.
1991, Pubmed
Archer,
Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter.
1991,
Pubmed
Bohr,
DNA repair in an active gene: removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall.
1985,
Pubmed
Cosman,
Solution conformation of the major adduct between the carcinogen (+)-anti-benzo[a]pyrene diol epoxide and DNA.
1992,
Pubmed
de los Santos,
Influence of benzo[a]pyrene diol epoxide chirality on solution conformations of DNA covalent adducts: the (-)-trans-anti-[BP]G.C adduct structure and comparison with the (+)-trans-anti-[BP]G.C enantiomer.
1992,
Pubmed
DiGiovanni,
Formation and disappearance of benzo[a]pyrene DNA-adducts in mouse epidermis.
1985,
Pubmed
Franklin,
Structural determination of the ultraviolet light-induced thymine-cytosine pyrimidine-pyrimidone (6-4) photoproduct.
1985,
Pubmed
Gale,
UV-induced formation of pyrimidine dimers in nucleosome core DNA is strongly modulated with a period of 10.3 bases.
1987,
Pubmed
Gale,
UV induced (6-4) photoproducts are distributed differently than cyclobutane dimers in nucleosomes.
1990,
Pubmed
Hayes,
Preferential and asymmetric interaction of linker histones with 5S DNA in the nucleosome.
1993,
Pubmed
,
Xenbase
Hayes,
Histone contributions to the structure of DNA in the nucleosome.
1991,
Pubmed
,
Xenbase
Hogan,
Carcinogen-induced alteration of DNA structure.
1981,
Pubmed
Jayasena,
Competitive nucleosome reconstitution of polydeoxynucleotides containing oligoguanosine tracts.
1989,
Pubmed
Kim,
Contrasting structural impacts induced by cis-syn cyclobutane dimer and (6-4) adduct in DNA duplex decamers: implication in mutagenesis and repair activity.
1995,
Pubmed
Libertini,
Histone hyperacetylation. Its effects on nucleosome core particle transitions.
1988,
Pubmed
Liu,
A slot blot method for detection of ultraviolet photoproducts in DNA.
1995,
Pubmed
Low,
Echinomycin and distamycin induce rotation of nucleosome core DNA.
1986,
Pubmed
MacLeod,
Binding of the transcription factor, Sp1, to non-target sites in DNA modified by benzo[a]pyrene diol epoxide.
1995,
Pubmed
MacLeod,
A possible role in chemical carcinogenesis for epigenetic, heritable changes in gene expression.
1996,
Pubmed
MacLeod,
Interference of benzo[a]pyrene diol epoxide-deoxyguanosine adducts in a GC box with binding of the transcription factor Sp1.
1996,
Pubmed
Mao,
Synthesis and characterization of covalent adducts derived from the binding of benzo[a]pyrene diol expoxide to a -GGG- sequence in a deoxyoligonucleotide.
1995,
Pubmed
Matsumoto,
The effects of ultraviolet C on in vitro nucleosome assembly and stability.
1994,
Pubmed
Matsumoto,
Preferential inhibition of nucleosome assembly by ultraviolet-induced (6-4)photoproducts.
1995,
Pubmed
McGhee,
Reaction of nucleosome DNA with dimethyl sulfate.
1979,
Pubmed
McMurray,
Binding of ethidium bromide causes dissociation of the nucleosome core particle.
1986,
Pubmed
Mitchell,
Nonrandom induction of pyrimidine-pyrimidone (6-4) photoproducts in ultraviolet-irradiated human chromatin.
1990,
Pubmed
Montecino,
Changes in chromatin structure support constitutive and developmentally regulated transcription of the bone-specific osteocalcin gene in osteoblastic cells.
1996,
Pubmed
Moyer,
Site-specific aflatoxin B1 adduction of sequence-positioned nucleosome core particles.
1989,
Pubmed
Neubauer,
Analysis of nucleosome positioning by in vitro reconstitution.
1989,
Pubmed
Pearlman,
Molecular models for DNA damaged by photoreaction.
1985,
Pubmed
Pruss,
Nucleosomal anatomy--where are the histones?
1995,
Pubmed
Richmond,
Structure of the nucleosome core particle at 7 A resolution.
,
Pubmed
Roberts,
Nucleosomes reconstituted in vitro on mouse mammary tumor virus B region DNA occupy multiple translational and rotational frames.
1995,
Pubmed
Satchwell,
Sequence periodicities in chicken nucleosome core DNA.
1986,
Pubmed
Schieferstein,
Modulation of cyclobutane pyrimidine dimer formation in a positioned nucleosome containing poly(dA.dT) tracts.
1996,
Pubmed
Schild,
A nucleosome-dependent static loop potentiates estrogen-regulated transcription from the Xenopus vitellogenin B1 promoter in vitro.
1993,
Pubmed
,
Xenbase
Shrader,
Artificial nucleosome positioning sequences.
1989,
Pubmed
Slaga,
Marked differences in the skin tumor-initiating activities of the optical enantiomers of the diastereomeric benzo(a)pyrene 7,8-diol-9,10-epoxides.
1979,
Pubmed
Smith,
Covalent binding of the carcinogen benzo[a]pyrene diol epoxide to Xenopus laevis 5 S DNA reconstituted into nucleosomes.
1993,
Pubmed
,
Xenbase
Springer,
Influences of complex organic mixtures on tumor-initiating activity, DNA binding and adducts of benzo[a]pyrene.
1989,
Pubmed
Straub,
Identification of the major adducts formed by reaction of benzo(a)pyrene diol epoxide with DNA in vitro.
1977,
Pubmed
Suquet,
UV damage to DNA strongly influences its rotational setting on the histone surface of reconstituted nucleosomes.
1993,
Pubmed
Suquet,
Repair of UV-induced (6-4) photoproducts in nucleosome core DNA.
1995,
Pubmed
Taylor,
Solution-state structure of the Dewar pyrimidinone photoproduct of thymidylyl-(3'----5')-thymidine.
1988,
Pubmed
Thrall,
Nucleosome structure modulates benzo[a]pyrenediol epoxide adduct formation.
1994,
Pubmed
,
Xenbase
Thrall,
DNA polymerase, RNA polymerase and exonuclease activities on a DNA sequence modified by benzo[a]pyrene diolepoxide.
1992,
Pubmed
,
Xenbase
Tornaletti,
Slow repair of pyrimidine dimers at p53 mutation hotspots in skin cancer.
1994,
Pubmed
Treiber,
Cisplatin-DNA adducts are molecular decoys for the ribosomal RNA transcription factor hUBF (human upstream binding factor).
1994,
Pubmed
Wallrath,
Architectural variations of inducible eukaryotic promoters: preset and remodeling chromatin structures.
1994,
Pubmed
Wang,
Site-specific effect of thymine dimer formation on dAn.dTn tract bending and its biological implications.
1991,
Pubmed
Wei,
Site-specific rates of excision repair of benzo[a]pyrene diol epoxide adducts in the hypoxanthine phosphoribosyltransferase gene of human fibroblasts: correlation with mutation spectra.
1995,
Pubmed
Wolffe,
A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
1986,
Pubmed
,
Xenbase
Xu,
Stereochemistry-dependent bending in oligonucleotide duplexes induced by site-specific covalent benzo[a]pyrene diol epoxide-guanine lesions.
1995,
Pubmed
