Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
We present evidence that both corepressors SMRT and N-CoR exist in large protein complexes with estimated sizes of 1.5-2 MDa in HeLa nuclear extracts. Using a combination of conventional and immunoaffinity chromatography, we have successfully isolated a SMRT complex and identified histone deacetylase 3 (HDAC3) and transducin (beta)-like I (TBL1), a WD-40 repeat-containing protein, as the subunits of the purified SMRT complex. We show that the HDAC3-containing SMRT and N-CoR complexes can bind to unliganded thyroid hormone receptors (TRs) in vitro. We demonstrate further that in Xenopus oocytes, both SMRT and N-CoR also associate with HDAC3 in large protein complexes and that injection of antibodies against HDAC3 or SMRT/N-CoR led to a partial relief of repression by unliganded TR/RXR. These findings thus establish both SMRT and N-CoR complexes as bona fide HDAC-containing complexes and shed new light on the molecular pathways by which N-CoR and SMRT function in transcriptional repression.
Alland,
Role for N-CoR and histone deacetylase in Sin3-mediated transcriptional repression.
1997, Pubmed
Alland,
Role for N-CoR and histone deacetylase in Sin3-mediated transcriptional repression.
1997,
Pubmed
Baniahmad,
The tau 4 activation domain of the thyroid hormone receptor is required for release of a putative corepressor(s) necessary for transcriptional silencing.
1995,
Pubmed
Baniahmad,
Modular structure of a chicken lysozyme silencer: involvement of an unusual thyroid hormone receptor binding site.
1990,
Pubmed
Bassi,
X-linked late-onset sensorineural deafness caused by a deletion involving OA1 and a novel gene containing WD-40 repeats.
1999,
Pubmed
Casanova,
Functional evidence for ligand-dependent dissociation of thyroid hormone and retinoic acid receptors from an inhibitory cellular factor.
1994,
Pubmed
Chen,
A transcriptional co-repressor that interacts with nuclear hormone receptors.
1995,
Pubmed
Ciana,
Leukemic transformation by the v-ErbA oncoprotein entails constitutive binding to and repression of an erythroid enhancer in vivo.
1998,
Pubmed
Dhordain,
The LAZ3(BCL-6) oncoprotein recruits a SMRT/mSIN3A/histone deacetylase containing complex to mediate transcriptional repression.
1998,
Pubmed
Dong,
ebi regulates epidermal growth factor receptor signaling pathways in Drosophila.
1999,
Pubmed
Dressel,
Alien, a highly conserved protein with characteristics of a corepressor for members of the nuclear hormone receptor superfamily.
1999,
Pubmed
Emiliani,
Characterization of a human RPD3 ortholog, HDAC3.
1998,
Pubmed
Forrest,
Thyroid hormone receptor beta is essential for development of auditory function.
1996,
Pubmed
Glass,
Positive and negative regulation of gene transcription by a retinoic acid-thyroid hormone receptor heterodimer.
1989,
Pubmed
Glass,
The coregulator exchange in transcriptional functions of nuclear receptors.
2000,
Pubmed
Grozinger,
Three proteins define a class of human histone deacetylases related to yeast Hda1p.
1999,
Pubmed
Gu,
Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain.
1997,
Pubmed
Hebbes,
A direct link between core histone acetylation and transcriptionally active chromatin.
1988,
Pubmed
Heinzel,
A complex containing N-CoR, mSin3 and histone deacetylase mediates transcriptional repression.
1997,
Pubmed
Hong,
SMRT corepressor interacts with PLZF and with the PML-retinoic acid receptor alpha (RARalpha) and PLZF-RARalpha oncoproteins associated with acute promyelocytic leukemia.
1997,
Pubmed
Hörlein,
Ligand-independent repression by the thyroid hormone receptor mediated by a nuclear receptor co-repressor.
1995,
Pubmed
Huang,
Nuclear receptor corepressors partner with class II histone deacetylases in a Sin3-independent repression pathway.
2000,
Pubmed
Kao,
A histone deacetylase corepressor complex regulates the Notch signal transduction pathway.
1998,
Pubmed
,
Xenbase
Kao,
Isolation of a novel histone deacetylase reveals that class I and class II deacetylases promote SMRT-mediated repression.
2000,
Pubmed
Kouzarides,
Histone acetylases and deacetylases in cell proliferation.
1999,
Pubmed
Lee,
A positive role for histone acetylation in transcription factor access to nucleosomal DNA.
1993,
Pubmed
,
Xenbase
Li,
p300 requires its histone acetyltransferase activity and SRC-1 interaction domain to facilitate thyroid hormone receptor activation in chromatin.
2000,
Pubmed
,
Xenbase
McKenna,
Nuclear receptor coregulators: cellular and molecular biology.
1999,
Pubmed
Nagy,
Nuclear receptor repression mediated by a complex containing SMRT, mSin3A, and histone deacetylase.
1997,
Pubmed
Nawaz,
The Angelman syndrome-associated protein, E6-AP, is a coactivator for the nuclear hormone receptor superfamily.
1999,
Pubmed
Ordentlich,
Unique forms of human and mouse nuclear receptor corepressor SMRT.
1999,
Pubmed
Park,
SMRTe, a silencing mediator for retinoid and thyroid hormone receptors-extended isoform that is more related to the nuclear receptor corepressor.
1999,
Pubmed
Parthun,
The major cytoplasmic histone acetyltransferase in yeast: links to chromatin replication and histone metabolism.
1996,
Pubmed
Qin,
A strategy for rapid, high-confidence protein identification.
1997,
Pubmed
Turner,
Histone acetylation in chromatin and chromosomes.
1995,
Pubmed
Wang,
Purification and biochemical heterogeneity of the mammalian SWI-SNF complex.
1996,
Pubmed
Wang,
ETO, fusion partner in t(8;21) acute myeloid leukemia, represses transcription by interaction with the human N-CoR/mSin3/HDAC1 complex.
1998,
Pubmed
Wolffe,
Transcription: in tune with the histones.
1994,
Pubmed
Wong,
Distinct requirements for chromatin assembly in transcriptional repression by thyroid hormone receptor and histone deacetylase.
1998,
Pubmed
,
Xenbase
Wong,
A role for nucleosome assembly in both silencing and activation of the Xenopus TR beta A gene by the thyroid hormone receptor.
1995,
Pubmed
,
Xenbase
Xue,
NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities.
1998,
Pubmed
Yang,
Isolation and characterization of cDNAs corresponding to an additional member of the human histone deacetylase gene family.
1997,
Pubmed
Zamir,
A nuclear hormone receptor corepressor mediates transcriptional silencing by receptors with distinct repression domains.
1996,
Pubmed
Zhang,
Histone deacetylases and SAP18, a novel polypeptide, are components of a human Sin3 complex.
1997,
Pubmed
Zhang,
Proteasomal regulation of nuclear receptor corepressor-mediated repression.
1998,
Pubmed
