Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2000 Aug 01;1915:4144-53. doi: 10.1093/emboj/19.15.4144.
Show Gene links
Show Anatomy links
Developmentally regulated, alternative splicing of the Rpn10 gene generates multiple forms of 26S proteasomes.
Kawahara H
,
Kasahara M
,
Nishiyama A
,
Ohsumi K
,
Goto T
,
Kishimoto T
,
Saeki Y
,
Yokosawa H
,
Shimbara N
,
Murata S
,
Chiba T
,
Suzuki K
,
Tanaka K
.
???displayArticle.abstract???
The 26S proteasome is a multisubunit protein- destroying machinery that degrades ubiquitin-tagged proteins. To date only a single species of Rpn10, which possibly functions as a multiubiquitin chain-binding subunit, has been identified in various organisms. Here we report that mouse Rpn10 mRNAs occur in at least five distinct forms, named Rpn10a to Rpn10e, and that they are generated from a single gene by developmentally regulated, alternative splicing. Rpn10a is ubiquitously expressed, whereas Rpn10e is expressed only in embryos, with the highest levels of expression in the brain. Both forms of Rpn10 are components of the 26S proteasome, with an apparently similar affinity for multiubiquitylated [(125)I]lysozyme in vitro. However, they exert markedly divergent effects on the destruction of B-type cyclin in Xenopus egg extracts. Thus, the 26S proteasome occurs in at least two functionally distinct forms: one containing a ubiquitously expressed Rpn10a and the other a newly identified, embryo-specific Rpn10e. While the former is thought to perform proteolysis constitutively in a wide variety of cells, the latter may play a specialized role in early embryonic development.
Bochtler,
The proteasome.
1999,
Pubmed
Braun,
The base of the proteasome regulatory particle exhibits chaperone-like activity.
1999,
Pubmed
Coux,
Structure and functions of the 20S and 26S proteasomes.
1996,
Pubmed
DeMartino,
The proteasome, a novel protease regulated by multiple mechanisms.
1999,
Pubmed
Deveraux,
Inhibition of ubiquitin-mediated proteolysis by the Arabidopsis 26 S protease subunit S5a.
1995,
Pubmed
,
Xenbase
Deveraux,
A 26 S protease subunit that binds ubiquitin conjugates.
1994,
Pubmed
Ferrell,
Molecular cloning and expression of a multiubiquitin chain binding subunit of the human 26S protease.
1996,
Pubmed
Ferrell,
Regulatory subunit interactions of the 26S proteasome, a complex problem.
2000,
Pubmed
Finley,
Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle.
1998,
Pubmed
Fu,
Multiubiquitin chain binding and protein degradation are mediated by distinct domains within the 26 S proteasome subunit Mcb1.
1998,
Pubmed
Girod,
Multiubiquitin chain binding subunit MCB1 (RPN10) of the 26S proteasome is essential for developmental progression in Physcomitrella patens.
1999,
Pubmed
Glickman,
A subcomplex of the proteasome regulatory particle required for ubiquitin-conjugate degradation and related to the COP9-signalosome and eIF3.
1998,
Pubmed
Haracska,
Cloning and sequencing a non-ATPase subunit of the regulatory complex of the Drosophila 26S protease.
1995,
Pubmed
Haracska,
Mapping the ubiquitin-binding domains in the p54 regulatory complex subunit of the Drosophila 26S protease.
1997,
Pubmed
Hartley,
In vivo regulation of the early embryonic cell cycle in Xenopus.
1996,
Pubmed
,
Xenbase
Hendil,
Simultaneous binding of PA28 and PA700 activators to 20 S proteasomes.
1998,
Pubmed
Hershko,
The ubiquitin system.
1998,
Pubmed
Kanayama,
Demonstration that a human 26S proteolytic complex consists of a proteasome and multiple associated protein components and hydrolyzes ATP and ubiquitin-ligated proteins by closely linked mechanisms.
1992,
Pubmed
Kominami,
Yeast counterparts of subunits S5a and p58 (S3) of the human 26S proteasome are encoded by two multicopy suppressors of nin1-1.
1997,
Pubmed
Kumatori,
Abnormally high expression of proteasomes in human leukemic cells.
1990,
Pubmed
Lopez,
Alternative splicing of pre-mRNA: developmental consequences and mechanisms of regulation.
1998,
Pubmed
Lorca,
Calmodulin-dependent protein kinase II mediates inactivation of MPF and CSF upon fertilization of Xenopus eggs.
1993,
Pubmed
,
Xenbase
Noguchi,
The MHC class I-like Zn-alpha 2-glycoprotein gene maps to mouse chromosome 5.
1995,
Pubmed
Pickart,
Targeting of substrates to the 26S proteasome.
1997,
Pubmed
Pusch,
Molecular cloning and testicular expression of the gene transcripts encoding the murine multiubiquitin-chain-binding protein (Mcb1).
1998,
Pubmed
Realini,
KEKE motifs. Proposed roles in protein-protein association and presentation of peptides by MHC class I receptors.
1994,
Pubmed
Rechsteiner,
The proteasome activator 11 S REG (PA28) and class I antigen presentation.
2000,
Pubmed
Rowe,
Maps from two interspecific backcross DNA panels available as a community genetic mapping resource.
1994,
Pubmed
Schwartz,
The ubiquitin-proteasome pathway and pathogenesis of human diseases.
1999,
Pubmed
Tanahashi,
Hybrid proteasomes. Induction by interferon-gamma and contribution to ATP-dependent proteolysis.
2000,
Pubmed
Tanaka,
Molecular biology of the proteasome.
1998,
Pubmed
Tanaka,
The MHC class I ligand-generating system: roles of immunoproteasomes and the interferon-gamma-inducible proteasome activator PA28.
1998,
Pubmed
Thrower,
Recognition of the polyubiquitin proteolytic signal.
2000,
Pubmed
van Nocker,
The multiubiquitin-chain-binding protein Mcb1 is a component of the 26S proteasome in Saccharomyces cerevisiae and plays a nonessential, substrate-specific role in protein turnover.
1996,
Pubmed
van Nocker,
Arabidopsis MBP1 gene encodes a conserved ubiquitin recognition component of the 26S proteasome.
1996,
Pubmed
Voges,
The 26S proteasome: a molecular machine designed for controlled proteolysis.
1999,
Pubmed
Wilkinson,
Analysis of a gene encoding Rpn10 of the fission yeast proteasome reveals that the polyubiquitin-binding site of this subunit is essential when Rpn12/Mts3 activity is compromised.
2000,
Pubmed
Young,
Characterization of two polyubiquitin binding sites in the 26 S protease subunit 5a.
1998,
Pubmed
