Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nucleic Acids Res
2000 Aug 01;2815:2927-34. doi: 10.1093/nar/28.15.2927.
Show Gene links
Show Anatomy links
La protein has a positive effect on the translation of TOP mRNAs in vivo.
Crosio C
,
Boyl PP
,
Loreni F
,
Pierandrei-Amaldi P
,
Amaldi F
.
???displayArticle.abstract???
In vertebrates, the mRNAs encoding ribosomal proteins, as well as other proteins implicated in translation, are characterized by a 5'-untranslated region (5'-UTR), including a stretch of pyrimidines at the 5'-end. The 5'-terminal oligopyrimidine (5'-TOP) sequence, which is involved in the growth-dependent translational regulation characteristic of this class of genes (so-called TOP genes), has been shown to specifically bind the La protein in vitro, suggesting that La might be implicated in translational regulation in vivo. In order to substantiate this hypothesis, we have examined the effect of La on TOP mRNA translational control in both stable and transient transfection experiments. In particular we have constructed and analyzed three stably transfected Xenopus cell lines inducible for overexpression of wild-type La or of putative dominant negative mutated forms. Moreover, La-expressing plasmids have been transiently co-transfected together with a plasmid expressing a reporter TOP mRNA in a human cell line. Our results suggest that in vivo La protein plays a positive role in the translation of TOP mRNA. They also suggest that the function of La is to counteract translational repression exerted by a negative factor, possibly cellular nucleic acid binding protein (CNBP), which has been previously shown to bind the 5'-UTR downstream from the 5'-TOP sequence.
Ali,
The La antigen binds 5' noncoding region of the hepatitis C virus RNA in the context of the initiator AUG codon and stimulates internal ribosome entry site-mediated translation.
1997, Pubmed
Ali,
The La antigen binds 5' noncoding region of the hepatitis C virus RNA in the context of the initiator AUG codon and stimulates internal ribosome entry site-mediated translation.
1997,
Pubmed
Avni,
Vertebrate mRNAs with a 5'-terminal pyrimidine tract are candidates for translational repression in quiescent cells: characterization of the translational cis-regulatory element.
1994,
Pubmed
Birney,
Analysis of the RNA-recognition motif and RS and RGG domains: conservation in metazoan pre-mRNA splicing factors.
1993,
Pubmed
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase
Boelens,
Nuclear retention of RNA as a mechanism for localization.
1995,
Pubmed
,
Xenbase
Camacho-Vanegas,
Flat absorbance background for sucrose gradients.
1995,
Pubmed
Camacho-Vanegas,
Construction of Xenopus (B3.2) and human (HeLa) cell lines expressing the tetracycline-controlled transactivator (tTA).
1998,
Pubmed
,
Xenbase
Cardinali,
Interaction of proteins with the mRNA for ribosomal protein L1 in Xenopus: structural characterization of in vivo complexes and identification of proteins that bind in vitro to its 5'UTR.
1993,
Pubmed
,
Xenbase
Chan,
Ribonucleoprotein SS-B/La belongs to a protein family with consensus sequences for RNA-binding.
1989,
Pubmed
Chang,
Direct interactions between autoantigen La and human immunodeficiency virus leader RNA.
1994,
Pubmed
Chien,
Isolation and characterization of calmodulin genes from Xenopus laevis.
1984,
Pubmed
,
Xenbase
Craig,
The La autoantigen contains a dimerization domain that is essential for enhancing translation.
1997,
Pubmed
Fan,
Phosphorylation of the human La antigen on serine 366 can regulate recycling of RNA polymerase III transcription complexes.
1997,
Pubmed
Francoeur,
Interaction between VA RNA and the lupus antigen La: formation of a ribonucleoprotein particle in vitro.
1982,
Pubmed
Goodier,
A carboxy-terminal basic region controls RNA polymerase III transcription factor activity of human La protein.
1997,
Pubmed
Gossen,
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
1992,
Pubmed
Gottlieb,
The RNA binding protein La influences both the accuracy and the efficiency of RNA polymerase III transcription in vitro.
1989,
Pubmed
Gottlieb,
Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III.
1989,
Pubmed
Grimm,
In vivo selection of RNAs that localize in the nucleus.
1997,
Pubmed
,
Xenbase
Habets,
Characterization of the SS-B (La) antigen in adenovirus-infected and uninfected HeLa cells.
1983,
Pubmed
Hammond,
Sequences mediating the translation of mouse S16 ribosomal protein mRNA during myoblast differentiation and in vitro and possible control points for the in vitro translation.
1991,
Pubmed
James,
Translational control by the La antigen. Structure requirements for rescue of the double-stranded RNA-mediated inhibition of protein synthesis.
1999,
Pubmed
Jefferies,
Rapamycin suppresses 5'TOP mRNA translation through inhibition of p70s6k.
1997,
Pubmed
Jefferies,
Rapamycin selectively represses translation of the "polypyrimidine tract" mRNA family.
1994,
Pubmed
Kaspar,
A regulatory cis element and a specific binding factor involved in the mitogenic control of murine ribosomal protein L32 translation.
1992,
Pubmed
Krieg,
The mRNA encoding elongation factor 1-alpha (EF-1 alpha) is a major transcript at the midblastula transition in Xenopus.
1989,
Pubmed
,
Xenbase
Lerner,
Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus.
1981,
Pubmed
Levy,
Oligopyrimidine tract at the 5' end of mammalian ribosomal protein mRNAs is required for their translational control.
1991,
Pubmed
Lin-Marq,
Efficient synthesis, termination and release of RNA polymerase III transcripts in Xenopus extracts depleted of La protein.
1998,
Pubmed
,
Xenbase
Loreni,
Nucleotide sequence of the L1 ribosomal protein gene of Xenopus laevis: remarkable sequence homology among introns.
1985,
Pubmed
,
Xenbase
Madore,
Eukaryotic small ribonucleoproteins. Anti-La human autoantibodies react with U1 RNA-protein complexes.
1984,
Pubmed
Maraia,
Eukaryotic transcription termination factor La mediates transcript release and facilitates reinitiation by RNA polymerase III.
1994,
Pubmed
Maraia,
Transcription termination factor La is also an initiation factor for RNA polymerase III.
1996,
Pubmed
Mariottini,
Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis.
1993,
Pubmed
,
Xenbase
Mariottini,
The 5' untranslated region of mRNA for ribosomal protein S19 is involved in its translational regulation during Xenopus development.
1990,
Pubmed
,
Xenbase
McBratney,
Evidence for involvement of trans-acting factors in selection of the AUG start codon during eukaryotic translational initiation.
1996,
Pubmed
McLaren,
Human La protein: a stabilizer of histone mRNA.
1997,
Pubmed
Meerovitch,
La autoantigen enhances and corrects aberrant translation of poliovirus RNA in reticulocyte lysate.
1993,
Pubmed
Moskaitis,
Sequence of Xenopus laevis ferritin mRNA.
1990,
Pubmed
,
Xenbase
Peek,
Interaction of the La (SS-B) autoantigen with small ribosomal subunits.
1996,
Pubmed
Pellizzoni,
Involvement of the Xenopus laevis Ro60 autoantigen in the alternative interaction of La and CNBP proteins with the 5'UTR of L4 ribosomal protein mRNA.
1998,
Pubmed
,
Xenbase
Pellizzoni,
A Xenopus laevis homologue of the La autoantigen binds the pyrimidine tract of the 5' UTR of ribosomal protein mRNAs in vitro: implication of a protein factor in complex formation.
1996,
Pubmed
,
Xenbase
Pellizzoni,
Cellular nucleic acid binding protein binds a conserved region of the 5' UTR of Xenopus laevis ribosomal protein mRNAs.
1997,
Pubmed
,
Xenbase
Ruberti,
Characterization of histone genes isolated from Xenopus laevis and Xenopus tropicalis genomic libraries.
1982,
Pubmed
,
Xenbase
Sáfrány,
The relative contributions of various transcription factors to the overall promoter strength of the mouse ribosomal protein L30 gene.
1995,
Pubmed
Scherly,
La proteins from Xenopus laevis. cDNA cloning and developmental expression.
1993,
Pubmed
,
Xenbase
Seiler-Tuyns,
The human estrogen receptor can regulate exogenous but not endogenous vitellogenin gene promoters in a Xenopus cell line.
1988,
Pubmed
,
Xenbase
Simons,
The interactions with Ro60 and La differentially affect nuclear export of hY1 RNA.
1996,
Pubmed
,
Xenbase
Stefano,
Purified lupus antigen La recognizes an oligouridylate stretch common to the 3' termini of RNA polymerase III transcripts.
1984,
Pubmed
Svitkin,
La autoantigen alleviates translational repression by the 5' leader sequence of the human immunodeficiency virus type 1 mRNA.
1994,
Pubmed
Svitkin,
General RNA binding proteins render translation cap dependent.
1996,
Pubmed
Terada,
Rapamycin selectively inhibits translation of mRNAs encoding elongation factors and ribosomal proteins.
1994,
Pubmed
van Venrooij,
Structure and function of La and Ro RNPs.
1993,
Pubmed
Wigler,
Transformation of mammalian cells with genes from procaryotes and eucaryotes.
1979,
Pubmed
Yoo,
The yeast La protein is required for the 3' endonucleolytic cleavage that matures tRNA precursors.
1997,
Pubmed
