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Identification of pontin52 as an interaction partner of the Wnt/Wg signal transducer beta-catenin implicated a role for this protein in Wnt signaling. Here we describe the isolation of two Xenopus homologs of pontin52, Xpontin and Xreptin, and report the first expression pattern of vertebrate pontin52 homologs. Whole-mount in situ hybridization studies reveal a strong expression of Xpontin in neural crest cells and in later stages in different gastrointestinal organs. Xreptin is also expressed in neural crest cells, in particular in a subpopulation that give raise to the adrenal medulla.
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10842076
???displayArticle.link???Mech Dev
Fig. 2. Whole-mount in situ hybridization analyzes of Xpontin (A–I) and Xreptin (J–Q). (A) Xpontin expression at stage 6. (B) Xpontin expression at stage 10. (C) Section of a stage 11 embryo showing Xpontin expression in the ectoderm and the involuted mesoderm. (D) At neurula stage Xpontin is expressed in the anteriorneuroectoderm. (E) Expression of Xpontin at stage 26 in neural crest cells. (F) Staining pattern in the head of a stage 30 embryo. (G) At stage 43 Xpontin transcripts are predominantly found in esophagus (o) and proctodeum (p). (H) Section of a stage 43 embryo showing expression in the gills (g) and the esophagus (o). (I) Staining of Xpontin transcripts in isolated gastrointestinal tract of stage 48 embryo is visible in the gall bladder (gb). (J) Xreptin expression at stage 6. (K) Section of a stage 9 embryo showing Xreptin expression in the animal cap, and (L) at stage 10. (M) Expression of Xreptin in premigratory neural crest cells at stage 15. (N) Expression of Xreptin in cephalic neural crest cells at stage 26, and (O) at stage 35. Arrows 1 and 2 in (O) indicate prominent staining in the adrenal medulla (1) and in an unknown structure (2). (P) Close up of (O). Levels of sections shown in (Q,R) are given. (Q,R) Transverse sections of a stage 35 embryo. The arrow head in (Q) indicates the pronephric tubulus which is not stained, and the arrow marks staining in the adrenomedullary cells.
ruvbl1 (RuvB-like 1) gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 26, lateral view, anteriorleft, dorsal up
ruvbl1 (Xpontin) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 10.
ruvbl1 (Xpontin) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 18.
ruvbl1 (Xpontin) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 26, anteriorleft.
ruvbl1 (Xpontin) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 43, lateral view.
ruvbl1 (Xpontin) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 43, coronal section.
ruvbl2 (Xreptin) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35, lateral view.
ruvbl2 (Xreptin) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35, anteriorleft.
ruvbl2 (Xreptin) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35, coronal section.
