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We describe a novel bioassay system that uses Xenopus embryonic myocytes (myoballs) to detect the release of acetylcholine from Drosophila CNS neurons. When a voltage-clamped Xenopus myoball was manipulated into contact with cultured Drosophila "giant" neurons, spontaneous synaptic current-like events were registered. These events were observed within seconds after contact and were blocked by curare and alpha-bungarotoxin, but not by TTX and Cd(2+), suggesting that they are caused by the spontaneous quantal release of acetylcholine (ACh). The secretion occurred not only at the growth cone, but also along the neurite and at the soma, with significantly different release parameters among various regions. The amplitude of these currents displayed a skewed distribution. These features are distinct from synaptic transmission at more mature synapses or autapses formed in this culture system and are reminiscent of the transmitter release process during early development in other preparations. The usefulness of this coculture system in studying presynaptic secretion mechanisms is illustrated by a series of studies on the cAMP pathway mutations, dunce (dnc) and PKA-RI, which disrupt a cAMP-specific phosphodiesterase and the regulatory subunit of cAMP-dependent protein kinase A, respectively. We found that these mutations affected the ACh current kinetics, but not the quantal ACh packet, and that the release frequency was greatly enhanced by repetitive neuronal activity in dnc, but not wild-type, growth cones. These results suggest that the cAMP pathway plays an important role in the activity-dependent regulation of transmitter release not only in mature synapses as previously shown, but also in developing nerve terminals before synaptogenesis.
Baines,
Electrophysiological development of central neurons in the Drosophila embryo.
1998, Pubmed
Baines,
Electrophysiological development of central neurons in the Drosophila embryo.
1998,
Pubmed
Bekkers,
Origin of variability in quantal size in cultured hippocampal neurons and hippocampal slices.
1990,
Pubmed
Bellen,
Maternal effects of general and regional specificity on embryos of Drosophila melanogaster caused by dunce and rutabaga mutant combinations.
1988,
Pubmed
Broadie,
Development of the embryonic neuromuscular synapse of Drosophila melanogaster.
1993,
Pubmed
Byers,
Defect in cyclic AMP phosphodiesterase due to the dunce mutation of learning in Drosophila melanogaster.
1981,
Pubmed
Cheung,
Drosophila larval neuromuscular junction's responses to reduction of cAMP in the nervous system.
1999,
Pubmed
Chow,
Release of acetylcholine from embryonic neurons upon contact with muscle cell.
1985,
Pubmed
,
Xenbase
Dai,
Elevation in presynaptic Ca2+ level accompanying initial nerve-muscle contact in tissue culture.
1993,
Pubmed
,
Xenbase
Davis,
Genetic dissection of structural and functional components of synaptic plasticity. III. CREB is necessary for presynaptic functional plasticity.
1996,
Pubmed
Davis,
Physiology and biochemistry of Drosophila learning mutants.
1996,
Pubmed
Delgado,
Outward currents in Drosophila larval neurons: dunce lacks a maintained outward current component downregulated by cAMP.
1998,
Pubmed
DeZazzo,
Dissection of memory formation: from behavioral pharmacology to molecular genetics.
1995,
Pubmed
Dudai,
dunce, a mutant of Drosophila deficient in learning.
1976,
Pubmed
Engel,
Altered habituation of an identified escape circuit in Drosophila memory mutants.
1996,
Pubmed
Evers,
Studies of nerve-muscle interactions in Xenopus cell culture: analysis of early synaptic currents.
1989,
Pubmed
,
Xenbase
Ganetzky,
Neurogenetic analysis of potassium currents in Drosophila: synergistic effects on neuromuscular transmission in double mutants.
1983,
Pubmed
Girod,
Spontaneous quantal transmitter secretion from myocytes and fibroblasts: comparison with neuronal secretion.
1995,
Pubmed
,
Xenbase
Goodwin,
Defective learning in mutants of the Drosophila gene for a regulatory subunit of cAMP-dependent protein kinase.
1997,
Pubmed
Hall,
Genetic analysis of Drosophila neurobiology.
1979,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Haydon,
Formation and modulation of chemical connections: evoked acetylcholine release from growth cones and neurites of specific identified neurons.
1989,
Pubmed
Hirling,
Phosphorylation of synaptic vesicle proteins: modulation of the alpha SNAP interaction with the core complex.
1996,
Pubmed
Huff,
Drosophila embryonic neuroblasts in culture: autonomous differentiation of specific neurotransmitters.
1989,
Pubmed
Hume,
Acetylcholine release from growth cones detected with patches of acetylcholine receptor-rich membranes.
,
Pubmed
Huttner,
Synapsin I (protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation.
1983,
Pubmed
Ikeda,
Patterned neural activity of a mutant Drosophila melanogaster.
1970,
Pubmed
Jan,
Two mutations of synaptic transmission in Drosophila.
1977,
Pubmed
Jan,
L-glutamate as an excitatory transmitter at the Drosophila larval neuromuscular junction.
1976,
Pubmed
Kelly,
Storage and release of neurotransmitters.
1993,
Pubmed
Keshishian,
The drosophila neuromuscular junction: a model system for studying synaptic development and function.
1996,
Pubmed
Kidokoro,
Changes in synaptic potential properties during acetylcholine receptor accumulation and neurospecific interactions in Xenopus nerve-muscle cell culture.
1980,
Pubmed
,
Xenbase
Kim,
Reduced growth cone motility in cultured neurons from Drosophila memory mutants with a defective cAMP cascade.
1996,
Pubmed
Lévêque,
Purification of the N-type calcium channel associated with syntaxin and synaptotagmin. A complex implicated in synaptic vesicle exocytosis.
1994,
Pubmed
Morimoto,
Calcium-dependent transmitter secretion from fibroblasts: modulation by synaptotagmin I.
1995,
Pubmed
,
Xenbase
O'Dowd,
Voltage-gated currents and firing properties of embryonic Drosophila neurons grown in a chemically defined medium.
1995,
Pubmed
Ohmori,
Development of neuromuscular transmission in a larval tunicate.
1977,
Pubmed
Pavlidis,
Seizures and failures in the giant fiber pathway of Drosophila bang-sensitive paralytic mutants.
1995,
Pubmed
Popov,
Synaptotagmin: a calcium-sensitive inhibitor of exocytosis?
1993,
Pubmed
Saito,
Expression of ion channels and mutational effects in giant Drosophila neurons differentiated from cell division-arrested embryonic neuroblasts.
1991,
Pubmed
Saito,
Ionic channels in cultured Drosophila neurons.
1993,
Pubmed
Salvaterra,
In vitro neuronal differentiation of Drosophila embryo cells.
1987,
Pubmed
Schuster,
Genetic dissection of structural and functional components of synaptic plasticity. II. Fasciclin II controls presynaptic structural plasticity.
1996,
Pubmed
Seecof,
Differentiation of neurons and myocytes in cell cultures made from Drosophila gastrulae.
1971,
Pubmed
Song,
Expression of a putative vesicular acetylcholine transporter facilitates quantal transmitter packaging.
1997,
Pubmed
,
Xenbase
Spitzer,
The development of the action potential mechanism of amphibian neurons isolated in culture.
1976,
Pubmed
,
Xenbase
Südhof,
The synaptic vesicle cycle: a cascade of protein-protein interactions.
1995,
Pubmed
Sun,
Evoked release of acetylcholine from the growing embryonic neuron.
1987,
Pubmed
,
Xenbase
Takagawa,
Analysis of choline acetyltransferase protein in temperature sensitive mutant flies using newly generated monoclonal antibody.
1996,
Pubmed
Tanouye,
Abnormal action potentials associated with the Shaker complex locus of Drosophila.
1981,
Pubmed
Thomas,
Mutations altering synaptic connectivity between identified neurons in Drosophila.
1984,
Pubmed
Trimarchi,
The shaking-B2 mutation disrupts electrical synapses in a flight circuit in adult Drosophila.
1997,
Pubmed
Tully,
Classical conditioning and retention in normal and mutant Drosophila melanogaster.
1985,
Pubmed
Tully,
A return to genetic dissection of memory in Drosophila.
1996,
Pubmed
Wang,
Concomitant alterations of physiological and developmental plasticity in Drosophila CaM kinase II-inhibited synapses.
1994,
Pubmed
Weber,
SNAREpins: minimal machinery for membrane fusion.
1998,
Pubmed
Wu,
Giant Drosophila neurons differentiated from cytokinesis-arrested embryonic neuroblasts.
1990,
Pubmed
Wu,
Genetic dissection of synaptic transmission in Drosophila.
1997,
Pubmed
Wu,
Dissociated neurons from normal and mutant Drosophila larval central nervous system in cell culture.
1983,
Pubmed
Xie,
Initial events in the formation of neuromuscular synapse: rapid induction of acetylcholine release from embryonic neuron.
1986,
Pubmed
,
Xenbase
Yao,
Auxiliary Hyperkinetic beta subunit of K+ channels: regulation of firing properties and K+ currents in Drosophila neurons.
1999,
Pubmed
Young,
Spontaneous release of transmitter from growth cones of embryonic neurones.
,
Pubmed
,
Xenbase
Zhao,
Functional expression of Shaker K+ channels in cultured Drosophila "giant" neurons derived from Sh cDNA transformants: distinct properties, distribution, and turnover.
1995,
Pubmed
,
Xenbase
Zhao,
Alterations in frequency coding and activity dependence of excitability in cultured neurons of Drosophila memory mutants.
1997,
Pubmed
Zhong,
Synaptic plasticity in Drosophila memory and hyperexcitable mutants: role of cAMP cascade.
1992,
Pubmed
Zhong,
Differential modulation of potassium currents by cAMP and its long-term and short-term effects: dunce and rutabaga mutants of Drosophila.
1993,
Pubmed
Zhong,
Mediation of PACAP-like neuropeptide transmission by coactivation of Ras/Raf and cAMP signal transduction pathways in Drosophila.
1995,
Pubmed
Zhong,
Altered synaptic plasticity in Drosophila memory mutants with a defective cyclic AMP cascade.
1991,
Pubmed
