Mueller-Pillasch F , Pohl B , Wilda M , Lacher U , Beil M , Wallrapp C , Hameister H , Knöchel W , Adler G , Gress TM .

Fig. 3. Temporal and spatial expression of Xenopus Vg1-RBP. RT-PCR of Vg1-RBP transcripts in Xenopus embryos at different developmental stages (classification according to Nieuwkoop and Faber, 1975) was performed using a specific set of Vg1-RBP primers (upper). Transcription of the ubiquitous histone H4 gene served as an internal control. Whole mount in situ hybridization (lower) was carried out with digoxygenin labeled antisense RNA at different developmental stages (Nieuwkoop and Faber, 1975). (A) vegetal view, st. 10; (B) dorsal, st. 21; (C) lateral and dorsal, st. 27; (D) lateral, st.32; (E) lateral, st. 36; (F) lateral, st. 42. (G-O) transverse vibratome sections (50 mm) of whole mount stained embryos showing Vg1-RBP expression in trunk neural crest cells (G-L; st. 15 to st. 27), pronephric tubules (M, st. 34) and within the eye in the region of the forming lens (N and O, st. 34/35). (P) in situ hybridization using a horizontal section (10 mm) of a st. 45 embryo and [35S] rUTP labeled Vg1-RBP antisense RNA as probe. Anterior of the embryo is to the right, bright field is upper, dark field is bottom. a: archenteron, ba: branchial arch, bl: blood islands, cg: cement gland, dl: dorsal lip, e: eye, fb: forebrain, g: gut, hg: hindgut, hmc: head mesenchyme, l: lens, ld: liver diverticulum, ms: mesencephalon, n: notochord, nc: neural crest cells, nf: neural fold, np: neural plate, nt: neural tube, pa: pancreas, pnt: pronephros tubules, rh: rhombencephalon, ov: otic vesicle.