Köster M et al. (1999), Genomic structure and embryonic expression of t...

XB-ART-12133

Mech Dev 1999 Oct 01;881:89-93. doi: 10.1016/s0925-4773(99)00159-8.

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Genomic structure and embryonic expression of the Xenopus winged helix factors XFD-13/13'.

Köster M , Dillinger K , Knöchel W .

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We have isolated the gene, its corresponding cDNA and a closely related cDNA encoding the Xenopus winged helix factors XFD-13' and XFD-13, respectively. XFD-13/13' are regarded as pseudo-alleles and, based upon a comparison of sequences and genomic structures, represent the Xenopus orthologues to mammalian FREAC-1/HFH-8. XFD-13/13' genes are not transcribed during oogenesis, zygotic transcription starts at late gastrula/early neurula and transcripts persist throughout embryogenesis. Expression is found within head derived neural crest cells and the dorsolateral plate (DLP). At later developmental stages, cell populations of the DLP migrate to the ventral region but exclude the most posterior part. Since they are subsequently found to accumulate in vessel like structures, we suggest that these cells represent hematopoietic/endothelial progenitor cells.

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Species referenced: Xenopus
Genes referenced: foxf1

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	Fig. 2. Temporal and spatial expression of XFD-13/13' during Xenopus development for the temporal pattern, total RNA was subjected to RT-PCR (upper panel). The first lane (Oo) corresponds to RNA from mature oocytes, the last lane to a control assay lacking RNA. Histone H4 is used as an internal control. Stage classification is done according to Nieuwkoop and Faber (1975). The spatial pattern was analysed by whole mount in situ hybridizations. Embryos at stages 19 (A), 23 (B), 26 (C), 27 (D), 29 (E), 31(F), 36 (G), 39 (H) and 41 (I) and transversal sections of embryos at stages 24 (J), 32 (K) and 39 (L and M(higher magnification)) are shown. A horizontal section through a stage 32/33 embryo is shown in (N). dlp, dorsolateral plate; lb, lateral border of neural plate; n,notochord; nc, neural crest cells; v, vessel-like structures.
	foxf1 (forkhead box F1 ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28/29, lateral view, anterior left, dorsal up.
	foxf1 (forkhead box F1 ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 39, lateral view, anterior left, dorsal up.