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???displayArticle.abstract??? CDC7 is an essential gene required for DNA replication in Saccharomyces cerevisiae. Cdc7p homologs have recently been identified in vertebrates, but their role in DNA replication has not yet been addressed. Here we show that antibodies to the Xenopus laevis homolog, xCdc7, interfere with DNA replication in vivo in developing embryos and in vitro in cycling egg extracts. We also demonstrate cell cycle-dependent association of xCdc7 with the Mcm complex, which binds to replication origins and also is required for DNA synthesis. Taken together, these data indicate that the function of xCdc7 is conserved from fungi to vertebrates. xCdc7 protein accumulates after stimulation of resting oocytes with progesterone, suggesting a molecular explanation for previous observations that the development of the capacity for DNA replication requires protein synthesis late in meiosis I.
Aladjem,
Genetic dissection of a mammalian replicator in the human beta-globin locus.
1998, Pubmed
Aladjem,
Genetic dissection of a mammalian replicator in the human beta-globin locus.
1998,
Pubmed
Anderson,
Ionizing radiation induces apoptosis and elevates cyclin A1-Cdk2 activity before but not after the midblastula transition in Xenopus.
1997,
Pubmed
,
Xenbase
Anderson,
Processing of adenovirus 2-induced proteins.
1973,
Pubmed
Aparicio,
Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
1997,
Pubmed
Bartek,
The retinoblastoma protein pathway in cell cycle control and cancer.
1997,
Pubmed
Benbow,
Cytoplasmic control of nuclear DNA synthesis during early development of Xenopus laevis: a cell-free assay.
1975,
Pubmed
,
Xenbase
Blumenthal,
The units of DNA replication in Drosophila melanogaster chromosomes.
1974,
Pubmed
Bousset,
The Cdc7 protein kinase is required for origin firing during S phase.
1998,
Pubmed
Carpenter,
Role for a Xenopus Orc2-related protein in controlling DNA replication.
1996,
Pubmed
,
Xenbase
Cocker,
An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast.
1996,
Pubmed
Coleman,
The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts.
1996,
Pubmed
,
Xenbase
Donaldson,
Cdc7 is required throughout the yeast S phase to activate replication origins.
1998,
Pubmed
Epstein,
CLB5: a novel B cyclin from budding yeast with a role in S phase.
1992,
Pubmed
Foss,
Origin recognition complex (ORC) in transcriptional silencing and DNA replication in S. cerevisiae.
1993,
Pubmed
Furuno,
Suppression of DNA replication via Mos function during meiotic divisions in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Gautier,
Cyclin is a component of maturation-promoting factor from Xenopus.
1990,
Pubmed
,
Xenbase
Gurdon,
The appearance of cytoplasmic DNA polymerase activity during the maturation of amphibian oocytes into eggs.
1969,
Pubmed
Gurdon,
On the origin and persistence of a cytoplasmic state inducing nuclear DNA synthesis in frogs' eggs.
1967,
Pubmed
Hanks,
Protein kinase catalytic domain sequence database: identification of conserved features of primary structure and classification of family members.
1991,
Pubmed
Hardy,
mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p.
1997,
Pubmed
Hartley,
A role for cyclin E/Cdk2 in the timing of the midblastula transition in Xenopus embryos.
1997,
Pubmed
,
Xenbase
Haun,
Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors.
1992,
Pubmed
Hess,
A human homolog of the yeast CDC7 gene is overexpressed in some tumors and transformed cell lines.
1998,
Pubmed
Hyrien,
Transition in specification of embryonic metazoan DNA replication origins.
1995,
Pubmed
,
Xenbase
Jiang,
Identification and characterization of a human protein kinase related to budding yeast Cdc7p.
1997,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lei,
Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis.
1997,
Pubmed
Madine,
MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells.
1995,
Pubmed
,
Xenbase
Marx,
How DNA replication originates.
1995,
Pubmed
Mendenhall,
An inhibitor of p34CDC28 protein kinase activity from Saccharomyces cerevisiae.
1993,
Pubmed
Micklem,
Yeast origin recognition complex is involved in DNA replication and transcriptional silencing.
1993,
Pubmed
Murray,
Cell cycle extracts.
1991,
Pubmed
Nasmyth,
At the heart of the budding yeast cell cycle.
1996,
Pubmed
Nugroho,
An inhibitor of yeast cyclin-dependent protein kinase plays an important role in ensuring the genomic integrity of daughter cells.
1994,
Pubmed
Philpott,
E2F and its developmental regulation in Xenopus laevis.
1994,
Pubmed
,
Xenbase
Romanowski,
The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin.
1996,
Pubmed
,
Xenbase
Roy,
Mos proto-oncogene function during oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase
Sato,
Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7.
1997,
Pubmed
,
Xenbase
Schneider,
Linkage of replication to start by the Cdk inhibitor Sic1.
1996,
Pubmed
Schwob,
The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae.
1994,
Pubmed
Sherman,
Schizosaccharomyces pombe Mcm3p, an essential nuclear protein, associates tightly with Nda4p (Mcm5p).
1998,
Pubmed
Sherman,
Multiple domains of fission yeast Cdc19p (MCM2) are required for its association with the core MCM complex.
1998,
Pubmed
Sible,
Developmental regulation of MCM replication factors in Xenopus laevis.
1998,
Pubmed
,
Xenbase
Strausfeld,
Both cyclin A and cyclin E have S-phase promoting (SPF) activity in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase
Toone,
Getting started: regulating the initiation of DNA replication in yeast.
1997,
Pubmed
Walter,
Regulation of replicon size in Xenopus egg extracts.
1997,
Pubmed
,
Xenbase
