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Chloride dependence of hyperpolarization-activated chloride channel gates.
Pusch M
,
Jordt SE
,
Stein V
,
Jentsch TJ
.
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1. ClC proteins are a class of voltage-dependent Cl- channels with several members mutated in human diseases. The prototype ClC-0 Torpedo channel is a dimeric protein; each subunit forms a pore that can gate independently from the other one. A common slower gating mechanism acts on both pores simultaneously; slow gating activates ClC-0 at hyperpolarized voltages. The ClC-2 Cl- channel is also activated by hyperpolarization, as are some ClC-1 mutants (e.g. D136G) and wild-type (WT) ClC-1 at certain pH values. 2. We studied the dependence on internal Cl- ([Cl-]i) of the hyperpolarization-activated gates of several ClC channels (WT ClC-0, ClC-0 mutant P522G, ClC-1 mutant D136G and an N-terminal deletion mutant of ClC-2), by patch clamping channels expressed in Xenopus oocytes. 3. With all these channels, reducing [Cl-]i shifted activation to more negative voltages and reduced the maximal activation at most negative voltages. 4. We also investigated the external halide dependence of WT ClC-2 using two-electrode voltage-clamp recording. Reducing external Cl- ([Cl-]o) activated ClC-2 currents. Replacing [Cl-]o by the less permeant Br- reduced channel activity and accelerated deactivation. 5. Gating of the ClC-2 mutant K566Q in normal [Cl-]o resembled that of WT ClC-2 in low [Cl-]o, i.e. channels had a considerable open probability (Po) at resting membrane potential. Substituting external Cl- by Br- or I- led to a decrease in Po. 6. The [Cl-]i dependence of the hyperpolarization-activated gates of various ClC channels suggests a similar gating mechanism, and raises the possibility that the gating charge for the hyperpolarization-activated gate is provided by Cl-. 7. The external halide dependence of hyperpolarization-activated gating of ClC-2 suggests that it is mediated or modulated by anions as in other ClC channels. In contrast to the depolarization-activated fast gates of ClC-0 and ClC-1, the absence of Cl- favours channel opening. Lysine 556 may be important for the relevant binding site.
Carew,
Identification of ClC-2-like chloride currents in pig pancreatic acinar cells.
1996, Pubmed
Carew,
Identification of ClC-2-like chloride currents in pig pancreatic acinar cells.
1996,
Pubmed
Chen,
Nonequilibrium gating and voltage dependence of the ClC-0 Cl- channel.
1996,
Pubmed
Chesnoy-Marchais,
Characterization of a chloride conductance activated by hyperpolarization in Aplysia neurones.
1983,
Pubmed
Clark,
Characterization of the hyperpolarization-activated chloride current in dissociated rat sympathetic neurons.
1998,
Pubmed
,
Xenbase
Dinudom,
Na+ and Cl- conductances are controlled by cytosolic Cl- concentration in the intralobular duct cells of mouse mandibular glands.
1993,
Pubmed
Fahlke,
An aspartic acid residue important for voltage-dependent gating of human muscle chloride channels.
1995,
Pubmed
,
Xenbase
Fahlke,
Subunit stoichiometry of human muscle chloride channels.
1997,
Pubmed
,
Xenbase
Fahlke,
Pore-forming segments in voltage-gated chloride channels.
1997,
Pubmed
Fahlke,
Pore stoichiometry of a voltage-gated chloride channel.
1998,
Pubmed
Ferroni,
Characterization of an inwardly rectifying chloride conductance expressed by cultured rat cortical astrocytes.
1997,
Pubmed
Fong,
Determinants of slow gating in ClC-0, the voltage-gated chloride channel of Torpedo marmorata.
1998,
Pubmed
,
Xenbase
Foskett,
ClC and CFTR chloride channel gating.
1998,
Pubmed
Fritsch,
Modulation of the hyperpolarization-activated Cl- current in human intestinal T84 epithelial cells by phosphorylation.
1996,
Pubmed
Gründer,
Regions involved in the opening of CIC-2 chloride channel by voltage and cell volume.
,
Pubmed
,
Xenbase
Jentsch,
Primary structure of Torpedo marmorata chloride channel isolated by expression cloning in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Jentsch,
Chloride channels: a molecular perspective.
1996,
Pubmed
,
Xenbase
Jordt,
Molecular dissection of gating in the ClC-2 chloride channel.
1997,
Pubmed
,
Xenbase
Koch,
The skeletal muscle chloride channel in dominant and recessive human myotonia.
1992,
Pubmed
Lloyd,
A common molecular basis for three inherited kidney stone diseases.
1996,
Pubmed
,
Xenbase
Lorenz,
Heteromultimeric CLC chloride channels with novel properties.
1996,
Pubmed
,
Xenbase
Ludewig,
Inward rectification in ClC-0 chloride channels caused by mutations in several protein regions.
1997,
Pubmed
Ludewig,
Two physically distinct pores in the dimeric ClC-0 chloride channel.
1996,
Pubmed
,
Xenbase
Ludewig,
Analysis of a protein region involved in permeation and gating of the voltage-gated Torpedo chloride channel ClC-0.
1997,
Pubmed
,
Xenbase
Ludewig,
Independent gating of single pores in CLC-0 chloride channels.
1997,
Pubmed
Methfessel,
Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels.
1986,
Pubmed
,
Xenbase
Middleton,
Homodimeric architecture of a ClC-type chloride ion channel.
1996,
Pubmed
Miller,
Open-state substructure of single chloride channels from Torpedo electroplax.
1982,
Pubmed
Murray,
CIC-2: a developmentally dependent chloride channel expressed in the fetal lung and downregulated after birth.
1995,
Pubmed
Noda,
Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence.
,
Pubmed
Pusch,
Gating of the voltage-dependent chloride channel CIC-0 by the permeant anion.
1995,
Pubmed
,
Xenbase
Pusch,
Temperature dependence of fast and slow gating relaxations of ClC-0 chloride channels.
1997,
Pubmed
,
Xenbase
Pusch,
Knocking on channel's door. The permeating chloride ion acts as the gating charge in ClC-0.
1996,
Pubmed
Pusch,
Low single channel conductance of the major skeletal muscle chloride channel, ClC-1.
1994,
Pubmed
,
Xenbase
Reeves,
Cl- channels in basolateral renal medullary vesicles. IX. Channels from mouse MTAL cell patches and medullary vesicles.
1995,
Pubmed
Richard,
Steady-state coupling of ion-channel conformations to a transmembrane ion gradient.
1990,
Pubmed
Rychkov,
Permeation and block of the skeletal muscle chloride channel, ClC-1, by foreign anions.
1998,
Pubmed
,
Xenbase
Rychkov,
Concentration and pH dependence of skeletal muscle chloride channel ClC-1.
1996,
Pubmed
,
Xenbase
Simon,
Mutations in the chloride channel gene, CLCNKB, cause Bartter's syndrome type III.
1997,
Pubmed
Staley,
Alteration of GABAA receptor function following gene transfer of the CLC-2 chloride channel.
1996,
Pubmed
Staley,
The role of an inwardly rectifying chloride conductance in postsynaptic inhibition.
1994,
Pubmed
Steinmeyer,
Multimeric structure of ClC-1 chloride channel revealed by mutations in dominant myotonia congenita (Thomsen).
1994,
Pubmed
Steinmeyer,
Primary structure and functional expression of a developmentally regulated skeletal muscle chloride channel.
1991,
Pubmed
,
Xenbase
Stühmer,
Structural parts involved in activation and inactivation of the sodium channel.
1989,
Pubmed
,
Xenbase
Thakker,
Chloride channels cough up.
1997,
Pubmed
Thiemann,
A chloride channel widely expressed in epithelial and non-epithelial cells.
1992,
Pubmed
,
Xenbase
