Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The 60-kDa Ro autoantigen is normally complexed with small cytoplasmic RNAs known as Y RNAs. In Xenopus oocytes, the Ro protein is also complexed with a large class of variant 5S rRNA precursors that are folded incorrectly. Using purified baculovirus-expressed protein, we show that the 60-kDa Ro protein binds directly to both Y RNAs and misfolded 5S rRNA precursors. To understand how the protein recognizes these two distinct classes of RNAs, we investigated the features of Y RNA sequence and structure that are necessary for protein recognition. We identified a truncated Y RNA that is stably bound by the 60-kDa Ro protein. Within this 39-nt RNA is a conserved helix that is proposed to be the binding site for the Ro protein. Mutagenesis of this minimal Y RNA revealed that binding by the 60-kDa Ro protein requires specific base pairs within the conserved helix, a singly bulged nucleotide that disrupts the helix, and a three-nucleotide bulge on the opposing strand. Chemical probing experiments using diethyl pyrocarbonate demonstrated that, in the presence of the two bulges, the major groove of the conserved helix is accessible to protein side chains. These data are consistent with a model in which the Ro protein recognizes specific base pairs in the conserved helix by binding in the major groove of the RNA. Furthermore, experiments in which dimethyl sulfate was used to probe a naked and protein-bound Y RNA revealed that a structural alteration occurs in the RNA upon Ro protein binding.
Ben-Chetrit,
A 52-kD protein is a novel component of the SS-A/Ro antigenic particle.
1988, Pubmed
Ben-Chetrit,
A 52-kD protein is a novel component of the SS-A/Ro antigenic particle.
1988,
Pubmed
Black,
U5 small nuclear ribonucleoprotein: RNA structure analysis and ATP-dependent interaction with U4/U6.
1989,
Pubmed
Boire,
Purification of antigenically intact Ro ribonucleoproteins; biochemical and immunological evidence that the 52-kD protein is not a Ro protein.
1995,
Pubmed
Deutscher,
Molecular analysis of the 60-kDa human Ro ribonucleoprotein.
1988,
Pubmed
England,
Specific labeling of 3' termini of RNA with T4 RNA ligase.
1980,
Pubmed
Farris,
Genes for murine Y1 and Y3 Ro RNAs have class 3 RNA polymerase III promoter structures and are unlinked on mouse chromosome 6.
1996,
Pubmed
Farris,
Y3 is the most conserved small RNA component of Ro ribonucleoprotein complexes in vertebrate species.
1995,
Pubmed
,
Xenbase
Hendrick,
Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells.
1981,
Pubmed
Howe,
NMR studies of U1 snRNA recognition by the N-terminal RNP domain of the human U1A protein.
1994,
Pubmed
Kato,
Nucleotide sequence of 4.5S RNA (C8 or hY5) from HeLa cells.
1982,
Pubmed
Kelekar,
Molecular composition of Ro small ribonucleoprotein complexes in human cells. Intracellular localization of the 60- and 52-kD proteins.
1994,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1987,
Pubmed
Matera,
A perinucleolar compartment contains several RNA polymerase III transcripts as well as the polypyrimidine tract-binding protein, hnRNP I.
1995,
Pubmed
Melin,
Biochemical demonstration of complex formation of histone pre-mRNA with U7 small nuclear ribonucleoprotein and hairpin binding factors.
1992,
Pubmed
O'Brien,
A subset of hY RNAs is associated with erythrocyte Ro ribonucleoproteins.
1990,
Pubmed
O'Brien,
A possible role for the 60-kD Ro autoantigen in a discard pathway for defective 5S rRNA precursors.
1994,
Pubmed
,
Xenbase
O'Brien,
Xenopus Ro ribonucleoproteins: members of an evolutionarily conserved class of cytoplasmic ribonucleoproteins.
1993,
Pubmed
,
Xenbase
Peattie,
Chemical probes for higher-order structure in RNA.
1980,
Pubmed
Peattie,
Direct chemical method for sequencing RNA.
1979,
Pubmed
Pruijn,
Analysis of protein--RNA interactions within Ro ribonucleoprotein complexes.
1991,
Pubmed
Query,
A specific 31-nucleotide domain of U1 RNA directly interacts with the 70K small nuclear ribonucleoprotein component.
1989,
Pubmed
Seeman,
Sequence-specific recognition of double helical nucleic acids by proteins.
1976,
Pubmed
Shi,
A misfolded form of 5S rRNA is complexed with the Ro and La autoantigens.
1996,
Pubmed
,
Xenbase
Slobbe,
Ro ribonucleoprotein assembly in vitro. Identification of RNA-protein and protein-protein interactions.
1992,
Pubmed
Stern,
RNA-protein interactions in 30S ribosomal subunits: folding and function of 16S rRNA.
1989,
Pubmed
Uhlenbeck,
RNA structure comes of age.
1997,
Pubmed
van Gelder,
Common structural features of the Ro RNP associated hY1 and hY5 RNAs.
1994,
Pubmed
Van Horn,
Caenorhabditis elegans embryos contain only one major species of Ro RNP.
1995,
Pubmed
,
Xenbase
Weeks,
Major groove accessibility of RNA.
1993,
Pubmed
Weeks,
RNA recognition by Tat-derived peptides: interaction in the major groove?
1991,
Pubmed
Wolin,
The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs.
1984,
Pubmed
Wolin,
Genes for two small cytoplasmic Ro RNAs are adjacent and appear to be single-copy in the human genome.
1983,
Pubmed
