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In almost all animal species, immature oocytes are arrested naturally in the first meiotic prophase, with a large nucleus called the germinal vesicle. A number of previous studies showed that both activation of maturation/M phase-promoting factor (MPF) (assayed by semiquantitative cytological methods) and some other maturational events occur essentially normally in enucleated oocytes from many amphibian species and mice. Hence, for nearly three decades, it has generally been believed that nuclear material is dispensable for MPF activation and the meiotic cell cycle in vertebrate oocytes. Here, we have challenged this view by examining the histone H1 kinase activities and the molecular forms of MPF in experimentally manipulated Xenopus oocytes. We show that oocytes injected with nuclear material undergo much more rapid MPF activation and maturation than uninjected control oocytes. Conversely, enucleated oocytes, unlike nucleated counterparts, undergo only weak MPF activation in meiosis I and no detectable MPF reactivation in meiosis II, the latter accompanying inhibitory tyrosine phosphorylation of cdc2 kinase, the catalytic subunit of MPF. These results argue strongly that nuclear material is indispensable for the meiotic cell cycle, particularly MPF reactivation (or cdc2 tyrosine dephosphorylation) on entry into meiosis II, in Xenopus oocytes. The classical and general view may thus need reconsideration.
Balakier,
Cytoplasmic control of nuclear maturation in mouse oocytes.
1977, Pubmed
Balakier,
Cytoplasmic control of nuclear maturation in mouse oocytes.
1977,
Pubmed
Bałakier,
Chromosome condensation activity in the cytoplasm of anucleate and nucleate fragments of mouse oocytes.
1986,
Pubmed
Bałakier,
The role of germinal vesicle karyoplasm in the development of male pronucleus in the mouse.
1980,
Pubmed
Dabauvalle,
Role of nuclear material in the early cell cycle of Xenopus embryos.
1988,
Pubmed
,
Xenbase
Feldherr,
Nuclear protein synthesis in animal and vegetal hemispheres of Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed
,
Xenbase
Ford,
A method for enucleating oocytes of Xenopus laevis.
1977,
Pubmed
,
Xenbase
Furuno,
Suppression of DNA replication via Mos function during meiotic divisions in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Furuno,
Meiotic cell cycle in Xenopus oocytes is independent of cdk2 kinase.
1997,
Pubmed
,
Xenbase
Gard,
Microtubule organization during maturation of Xenopus oocytes: assembly and rotation of the meiotic spindles.
1992,
Pubmed
,
Xenbase
Gautier,
Cyclin B in Xenopus oocytes: implications for the mechanism of pre-MPF activation.
1991,
Pubmed
,
Xenbase
Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase
Hausen,
Distribution of nuclear proteins during maturation of the Xenopus oocyte.
1985,
Pubmed
,
Xenbase
Katagiri,
Spermatozoan response to the toad egg matured after removal of germinal vesicle.
1976,
Pubmed
Kishimoto,
Role of germinal vesicle material in producing maturation-promoting factor in starfish oocyte.
1981,
Pubmed
Kobayashi,
On the synthesis and destruction of A- and B-type cyclins during oogenesis and meiotic maturation in Xenopus laevis.
1991,
Pubmed
,
Xenbase
Kumagai,
Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts.
1996,
Pubmed
,
Xenbase
Laskey,
The role of nucleoplasmin in chromatin assembly and disassembly.
1993,
Pubmed
,
Xenbase
Lorca,
Dephosphorylation of cdc2 on threonine 161 is required for cdc2 kinase inactivation and normal anaphase.
1992,
Pubmed
,
Xenbase
Masui,
Oocyte maturation.
1979,
Pubmed
Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed
Masui,
Control of chromosome behavior during progesterone induced maturation of amphibian oocytes.
1979,
Pubmed
Miller,
The nuclear-cytoplasmic distribution of the Xenopus nuclear factor, xnf7, coincides with its state of phosphorylation during early development.
1991,
Pubmed
,
Xenbase
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
Okumura,
Initial triggering of M-phase in starfish oocytes: a possible novel component of maturation-promoting factor besides cdc2 kinase.
1996,
Pubmed
Ookata,
Relocation and distinct subcellular localization of p34cdc2-cyclin B complex at meiosis reinitiation in starfish oocytes.
1992,
Pubmed
Picard,
The role of the germinal vesicle in producing maturation-promoting factor (MPF) as revealed by the removal and transplantation of nuclear material in starfish oocytes.
1984,
Pubmed
Picard,
The cell cycle can occur in starfish oocytes and embryos without the production of transferable MPF (maturation-promoting factor).
1988,
Pubmed
Reynhout,
Studies on the appearance and nature of a maturation-inducing factor in the cytoplasm of amphibian oocytes exposed to progesterone.
1974,
Pubmed
,
Xenbase
Sagata,
What does Mos do in oocytes and somatic cells?
1997,
Pubmed
,
Xenbase
Sagata,
Meiotic metaphase arrest in animal oocytes: its mechanisms and biological significance.
1996,
Pubmed
Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase
Schorderet-Slatkine,
Progesterone induced maturation in oocytes of Xenopus laevis. Appearance of a 'maturation promoting factor' in enucleated oocytes.
1973,
Pubmed
,
Xenbase
Schuetz,
Local control mechanisms during oogenesis and folliculogenesis.
1985,
Pubmed
Skoblina,
The role of germinal vesicle in maturation of Pleurodeles waltlii oocytes induced by steroids.
1984,
Pubmed
Skoblina,
Independence of the cortex maturation from germinal vesicle material during the maturation of amphibian and sturgeon oocytes.
1969,
Pubmed
Smith,
Role of the oocyte nucleus in physiological maturation in Rana pipiens.
1969,
Pubmed
Solomon,
Role of phosphorylation in p34cdc2 activation: identification of an activating kinase.
1992,
Pubmed
,
Xenbase
Ziegler,
Control of chromosome behavior in amphibian oocytes. II. The effect of inhibitors of RNA and protein synthesis on the induction of chromosome condensation in transplanted brain nuclei by oocyte cytoplasm.
1976,
Pubmed
