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XB-ART-16022
Anal Biochem 1997 Sep 05;2512:158-62. doi: 10.1006/abio.1997.2260.
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Large-scale immunohistological staining using polyethylene glycol-embedded sections mounted on 96-well plates for monoclonal antibody screening.

Komiya T , Tanigawa Y , Oda S , Hirohashi S .


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We have developed a rapid, large-scale immunohistological staining method for monoclonal antibody screening. Sections embedded in polyethylene glycol are mounted on 96-well plates and then subjected to immunological procedures using a 96-well format. With this method, we can prepare 10 plates (960 samples) in 1 day and then perform immunohistological procedures in 1 day by using an enzyme-linked immunosorbent assay-like 96-well format. Thus, we can avoid difficulties seen in conventional immunohistological methods that use sections mounted on glass slides. This method offers high-throughput screening of monoclonal antibodies. In addition, it has the potential of being automated.

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