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We have isolated a Xenopus laevis genomic sequence distinct from, but sharing high sequence similarity with N-cadherin. We present evidence that the gene represented by this sequence, named XNcad3, resides at a separate locus to the two previously isolated N-cadherin clones from this species. Extensive analysis could detect no expression of XNcad3 in embryonic or adult tissues. It seems likely that XNcad3 represents a non-expressed pseudogene, or perhaps a novel cadherin with a restricted expression pattern.
Fig. 1. Blocks 1â10: multiple nucleotide sequence alignment of XNcad1, XNcad2, XNcad3 and XBNcad3 using the GCG package. The first base of the initiation codon is numbered as +1. Bases conserved between the sequences are boxed. Bottom block: multiple amino acid sequence alignment of the proteins encoded by XNcad1, XNcad2, XNcad3 and XBNcad3 using the GCG package. The alignment begins from the initiating methionine. Amino acids conserved between the sequences are boxed.
Fig. 2. PCR amplification of XNcad1, XNcad2 and XNcad3 sequences from the genomic DNA of an individual frog. Gene specific primers designed to the 5′ untranslated region of XNcad1,2 and 3 were used to amplify sequences from the genomic DNA of an individual frog. Bands of the predicted sizes were observed. No DNA controls were included for each set of primers.
Fig. 3. Expression of XNcad1 and XNcad3 in developing embryos. 20 μg of total RNA from a series of developing embryos were used in an RNase protection analysis with radiolabelled synthetic RNA gene specific probes as described in the text.
