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The cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride (Cl(-)) channel known to influence the function of other channels, including connexin channels. To further study potential functional interactions between CFTR and gap junction channels, we have co-expressed CFTR and connexin45 (Cx45) in Xenopus oocytes and monitored junctional conductance and voltage sensitivity by dual voltage clamp electrophysiology. In single oocytes expressing CFTR, an increase in cAMP caused by forskolin application induced a Cl(-) current and increased membrane conductance; application of diphenylamine carboxylic acid (CFTR blocker) readily blocked the Cl(-) current. With co-expression of CFTR and Cx45, application of forskolin to paired oocytes induced a typical outward current and increased junctional conductance (G(j)). In addition, the presence of CFTR reduced the transjunctional voltage sensitivity of Cx45 channels without affecting the kinetics of junctional current inactivation. The drop in voltage sensitivity was further enhanced by forskolin application. The data indicate that CFTR influences cell-to-cell coupling mediated by Cx45 channels.
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991, Pubmed,
Xenbase
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991,
Pubmed
,
Xenbase
Bear,
Cl- channel activity in Xenopus oocytes expressing the cystic fibrosis gene.
1991,
Pubmed
,
Xenbase
Bukauskas,
Coupling asymmetry of heterotypic connexin 45/ connexin 43-EGFP gap junctions: properties of fast and slow gating mechanisms.
2002,
Pubmed
Bukauskas,
Two distinct gating mechanisms in gap junction channels: CO2-sensitive and voltage-sensitive.
1997,
Pubmed
Chanson,
Defective regulation of gap junctional coupling in cystic fibrosis pancreatic duct cells.
1999,
Pubmed
Cheng,
Modulation of mature cystic fibrosis transmembrane regulator protein by the PDZ domain protein CAL.
2004,
Pubmed
Cunningham,
cAMP-stimulated ion currents in Xenopus oocytes expressing CFTR cRNA.
1992,
Pubmed
,
Xenbase
Di Stefano,
Diphenylamine-2-carboxylate, a blocker of the Cl(-)-conductive pathway in Cl(-)-transporting epithelia.
1985,
Pubmed
Drumm,
Chloride conductance expressed by delta F508 and other mutant CFTRs in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Elenes,
Heterotypic docking of Cx43 and Cx45 connexons blocks fast voltage gating of Cx43.
2001,
Pubmed
Gadsby,
Control of CFTR channel gating by phosphorylation and nucleotide hydrolysis.
1999,
Pubmed
Greger,
Cystic fibrosis and CFTR.
2001,
Pubmed
Haggie,
Increased diffusional mobility of CFTR at the plasma membrane after deletion of its C-terminal PDZ binding motif.
2004,
Pubmed
Harris,
Emerging issues of connexin channels: biophysics fills the gap.
2001,
Pubmed
Kausalya,
Connexin45 directly binds to ZO-1 and localizes to the tight junction region in epithelial MDCK cells.
2001,
Pubmed
Kunzelmann,
CFTR: interacting with everything?
2001,
Pubmed
Laing,
Connexin45 interacts with zonula occludens-1 in osteoblastic cells.
2001,
Pubmed
Lazrak,
Gap junction gating sensitivity to physiological internal calcium regardless of pH in Novikoff hepatoma cells.
1993,
Pubmed
Peracchia,
Effects of caffeine and ryanodine on low pHi-induced changes in gap junction conductance and calcium concentration in crayfish septate axons.
1990,
Pubmed
Peracchia,
Is the voltage gate of connexins CO2-sensitive? Cx45 channels and inhibition of calmodulin expression.
2003,
Pubmed
,
Xenbase
Peracchia,
Slow gating of gap junction channels and calmodulin.
2000,
Pubmed
,
Xenbase
Peracchia,
Is the chemical gate of connexins voltage sensitive? Behavior of Cx32 wild-type and mutant channels.
1999,
Pubmed
,
Xenbase
Peracchia,
Chemical gating of gap junction channels; roles of calcium, pH and calmodulin.
2004,
Pubmed
Peracchia,
Increase in gap junction resistance with acidification in crayfish septate axons is closely related to changes in intracellular calcium but not hydrogen ion concentration.
1990,
Pubmed
Peracchia,
Inhibition of calmodulin expression prevents low-pH-induced gap junction uncoupling in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Reczek,
Identification of EBP50: A PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family.
1997,
Pubmed
Reddy,
Effect of anion transport blockers on CFTR in the human sweat duct.
2002,
Pubmed
Schultz,
Pharmacology of CFTR chloride channel activity.
1999,
Pubmed
Schwiebert,
CFTR regulates outwardly rectifying chloride channels through an autocrine mechanism involving ATP.
1995,
Pubmed
Sheppard,
Structure and function of the CFTR chloride channel.
1999,
Pubmed
Spray,
Equilibrium properties of a voltage-dependent junctional conductance.
1981,
Pubmed
,
Xenbase
Stutts,
CFTR as a cAMP-dependent regulator of sodium channels.
1995,
Pubmed
Sugita,
CFTR Cl- channel and CFTR-associated ATP channel: distinct pores regulated by common gates.
1998,
Pubmed
Wang,
Accessory protein facilitated CFTR-CFTR interaction, a molecular mechanism to potentiate the chloride channel activity.
2000,
Pubmed
Wang,
Molecular studies of CFTR interacting proteins.
2001,
Pubmed
Yoo,
Assembly and trafficking of a multiprotein ROMK (Kir 1.1) channel complex by PDZ interactions.
2004,
Pubmed
,
Xenbase
Zhang,
Direct comparison of NPPB and DPC as probes of CFTR expressed in Xenopus oocytes.
2000,
Pubmed
,
Xenbase
