Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Oligomerization and maturation of Na,K-ATPase: functional interaction of the cytoplasmic NH2 terminus of the beta subunit with the alpha subunit.
Geering K
,
Beggah A
,
Good P
,
Girardet S
,
Roy S
,
Schaer D
,
Jaunin P
.
???displayArticle.abstract???
Subunit assembly plays an essential role in the maturation of oligomeric proteins. In this study, we have characterized the main structural and functional consequences of the assembly of alpha and beta subunits of Na,K-ATPase. Xenopus oocytes injected with alpha and/or beta cRNA were treated with brefeldin A, which permitted the accumulation of individual subunits or alpha-beta complexes in the ER. Only alpha subunits that are associated with beta subunits become resistant to trypsin digestion and cellular degradation. Similarly, assembly with beta subunits is necessary and probably sufficient for the catalytic alpha subunit to acquire its main functional properties at the level of the ER, namely the ability to adopt different ligand-dependent conformations and to hydrolyze ATP in an Na(+)- and K(+)-dependent, ouabain-inhibitable fashion. Not only the alpha but also the beta subunit undergoes a structural change after assembly, which results in a global increase in its protease resistance. Furthermore, extensive and controlled proteolysis assays on wild-type and NH2-terminally modified beta subunits revealed a K(+)-dependent interaction of the cytoplasmic NH2 terminus of the beta subunit with the alpha subunit, which is likely to be involved in the modulation of the K(+)-activation of the Na,K-pump transport activity. Thus, we conclude that the ER assembly process not only establishes the basic structural interactions between individual subunits, which are required for the maturation of oligomeric proteins, but also distinct, functional interactions, which are involved in the regulation of functional properties of mature proteins.
Ackermann,
Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport.
1990, Pubmed,
Xenbase
Ackermann,
Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport.
1990,
Pubmed
,
Xenbase
Beggah,
Hydrophobic C-terminal amino acids in the beta-subunit are involved in assembly with the alpha-subunit of Na,K-ATPase.
1993,
Pubmed
,
Xenbase
Blanco,
The alpha-subunit of the Na,K-ATPase has catalytic activity independent of the beta-subunit.
1994,
Pubmed
Chow,
Characterization of the beta-subunit of the H(+)-K(+)-ATPase using an inhibitory monoclonal antibody.
1993,
Pubmed
Doms,
Folding and assembly of viral membrane proteins.
1993,
Pubmed
Eakle,
The influence of beta subunit structure on the stability of Na+/K(+)-ATPase complexes and interaction with K+.
1994,
Pubmed
Fambrough,
Analysis of subunit assembly of the Na-K-ATPase.
1994,
Pubmed
Geering,
The functional role of the beta-subunit in the maturation and intracellular transport of Na,K-ATPase.
1991,
Pubmed
,
Xenbase
Geering,
A role for the beta-subunit in the expression of functional Na+-K+-ATPase in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Geering,
Subunit assembly and functional maturation of Na,K-ATPase.
1990,
Pubmed
Girardet,
Immunochemical evidence for a transmembrane orientation of both the (Na+, K+)-ATPase subunits.
1981,
Pubmed
Good,
Both VP2 and VP3 are synthesized from each of the alternative spliced late 19S RNA species of simian virus 40.
1988,
Pubmed
Good,
A nervous system-specific isotype of the beta subunit of Na+,K(+)-ATPase expressed during early development of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Green,
Acetylcholine receptor assembly: subunit folding and oligomerization occur sequentially.
1993,
Pubmed
Helenius,
The endoplasmic reticulum as a protein-folding compartment.
1992,
Pubmed
Homareda,
Location of signal sequences for membrane insertion of the Na+,K+-ATPase alpha subunit.
1989,
Pubmed
Jaisser,
Modulation of the Na,K-pump function by beta subunit isoforms.
1994,
Pubmed
,
Xenbase
Jaisser,
Primary sequence and functional expression of a novel ouabain-resistant Na,K-ATPase. The beta subunit modulates potassium activation of the Na,K-pump.
1992,
Pubmed
,
Xenbase
Jaunin,
Processing, intracellular transport, and functional expression of endogenous and exogenous alpha-beta 3 Na,K-ATPase complexes in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Jaunin,
Role of the transmembrane and extracytoplasmic domain of beta subunits in subunit assembly, intracellular transport, and functional expression of Na,K-pumps.
1993,
Pubmed
,
Xenbase
Jørgensen,
Structural basis for E1-E2 conformational transitions in Na,K-pump and Ca-pump proteins.
1988,
Pubmed
Jørgensen,
Proteolytic cleavage as a tool for studying structure and conformation of pure membrane-bound Na+, K+-ATPase.
1988,
Pubmed
Karlish,
Location of Asn831 of the alpha chain of Na/K-ATPase at the cytoplasmic surface. Implication for topological models.
1993,
Pubmed
Kawakami,
The transmembrane segment of the human Na,K-ATPase beta-subunit acts as the membrane incorporation signal.
1988,
Pubmed
Kolodziej,
Epitope tagging and protein surveillance.
1991,
Pubmed
Lippincott-Schwartz,
Microtubule-dependent retrograde transport of proteins into the ER in the presence of brefeldin A suggests an ER recycling pathway.
1990,
Pubmed
Lutsenko,
Molecular events in close proximity to the membrane associated with the binding of ligands to the Na,K-ATPase.
1994,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Nelson,
A general method of site-specific mutagenesis using a modification of the Thermus aquaticus polymerase chain reaction.
1989,
Pubmed
Pralong-Zamofing,
Regulation of alpha 1-beta 3-NA(+)-K(+)-ATPase isozyme during meiotic maturation of Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Renaud,
Cytoplasmic and transmembrane domain deletions of Na,K-ATPase beta-subunit. Effects on subunit assembly and intracellular transport.
1991,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schmalzing,
The adhesion molecule on glia (AMOG/beta 2) and alpha 1 subunits assemble to functional sodium pumps in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Schoner,
On the mechanism of Na+- and K+-stimulated hydrolysis of adenosine triphosphate. 1. Purification and properties of a Na+-and K+-activated ATPase from ox brain.
1967,
Pubmed
Shainskaya,
Evidence that the cation occlusion domain of Na/K-ATPase consists of a complex of membrane-spanning segments. Analysis of limit membrane-embedded tryptic fragments.
1994,
Pubmed
Shainskaya,
Chymotryptic digestion of the cytoplasmic domain of the beta subunit of Na/K-ATPase alters kinetics of occlusion of Rb+ ions.
1996,
Pubmed
Verrey,
Primary sequence of Xenopus laevis Na+-K+-ATPase and its localization in A6 kidney cells.
1989,
Pubmed
,
Xenbase
Zamofing,
Inhibition of N-glycosylation affects transepithelial Na+ but not Na+-K+-ATPase transport.
1989,
Pubmed
