Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
TPR proteins required for anaphase progression mediate ubiquitination of mitotic B-type cyclins in yeast.
Zachariae W
,
Nasmyth K
.
???displayArticle.abstract???
The abundance of B-type cyclin-CDK complexes is determined by regulated synthesis and degradation of cyclin subunits. Cyclin proteolysis is required for the final exit from mitosis and for the initiation of a new cell cycle. In extracts from frog or clam eggs, degradation is accompanied by ubiquitination of cyclin. Three genes, CDC16, CDC23, and CSE1 have recently been shown to be required specifically for cyclin B proteolysis in yeast. To test whether these genes are required for cyclin ubiquitination, we prepared extracts from G1-arrested yeast cells capable of conjugating ubiquitin to the B-type cyclin Clb2. The ubiquitination activity was cell cycle regulated, required Clb2's destruction box, and was low if not absent in cdc16, cdc23, cdc27, and cse1 mutants. Furthermore all these mutants were also defective in ubiquitination of another mitotic B-type cyclin, Clb3. The Cdc16, Cdc23, and Cdc27 proteins all contain several copies of the tetratricopeptide repeat and are subunits of a complex that is required for the onset of anaphase. The finding that gene products that are required for ubiquitination of Clb2 and Clb3 are also required for cyclin proteolysis in vivo provides the best evidence so far that cyclin B is degraded via the ubiquitin pathway in living cells. Xenopus homologues of Cdc16 and Cdc27 have meanwhile been shown to be associated with a 20S particle that appears to function as a cell cycle-regulated ubiquitin-protein ligase.
Adam,
The importance of importin.
1995,
Pubmed
,
Xenbase
Amon,
Closing the cell cycle circle in yeast: G2 cyclin proteolysis initiated at mitosis persists until the activation of G1 cyclins in the next cycle.
1994,
Pubmed
Byers,
Duplication of spindle plaques and integration of the yeast cell cycle.
1974,
Pubmed
Ciechanover,
The ubiquitin-proteasome proteolytic pathway.
1994,
Pubmed
Deshaies,
G1 cyclin-dependent activation of p34CDC28 (Cdc28p) in vitro.
1995,
Pubmed
Dohmen,
An essential yeast gene encoding a homolog of ubiquitin-activating enzyme.
1995,
Pubmed
Ellison,
Epitope-tagged ubiquitin. A new probe for analyzing ubiquitin function.
1991,
Pubmed
Epstein,
CLB5: a novel B cyclin from budding yeast with a role in S phase.
1992,
Pubmed
Félix,
Triggering of cyclin degradation in interphase extracts of amphibian eggs by cdc2 kinase.
1990,
Pubmed
,
Xenbase
Field,
Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.
1988,
Pubmed
Finley,
Inhibition of proteolysis and cell cycle progression in a multiubiquitination-deficient yeast mutant.
1994,
Pubmed
Fitch,
Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae.
1992,
Pubmed
Ghislain,
S. cerevisiae 26S protease mutants arrest cell division in G2/metaphase.
1993,
Pubmed
Gietz,
New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.
1988,
Pubmed
Glotzer,
Cyclin is degraded by the ubiquitin pathway.
1991,
Pubmed
,
Xenbase
Goebl,
The TPR snap helix: a novel protein repeat motif from mitosis to transcription.
1991,
Pubmed
Gordon,
Defective mitosis due to a mutation in the gene for a fission yeast 26S protease subunit.
1993,
Pubmed
Grandin,
Differential function and expression of Saccharomyces cerevisiae B-type cyclins in mitosis and meiosis.
1993,
Pubmed
Hershko,
Methylated ubiquitin inhibits cyclin degradation in clam embryo extracts.
1991,
Pubmed
Hershko,
Components of a system that ligates cyclin to ubiquitin and their regulation by the protein kinase cdc2.
1994,
Pubmed
Hochuli,
New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.
1987,
Pubmed
Holloway,
Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor.
1993,
Pubmed
,
Xenbase
Hoyt,
S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function.
1991,
Pubmed
Hunt,
The requirements for protein synthesis and degradation, and the control of destruction of cyclins A and B in the meiotic and mitotic cell cycles of the clam embryo.
1992,
Pubmed
Irniger,
Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast.
1995,
Pubmed
Jones,
Proteinase mutants of Saccharomyces cerevisiae.
1977,
Pubmed
King,
A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B.
1995,
Pubmed
,
Xenbase
Lamb,
Cdc16p, Cdc23p and Cdc27p form a complex essential for mitosis.
1994,
Pubmed
Li,
Feedback control of mitosis in budding yeast.
1991,
Pubmed
Lin,
The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosis.
1995,
Pubmed
Loeb,
The yeast nuclear import receptor is required for mitosis.
1995,
Pubmed
Luca,
Both cyclin A delta 60 and B delta 97 are stable and arrest cells in M-phase, but only cyclin B delta 97 turns on cyclin destruction.
1991,
Pubmed
,
Xenbase
Peters,
Proteasomes: protein degradation machines of the cell.
1994,
Pubmed
Pines,
Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport.
1991,
Pubmed
Schweitzer,
CDC15, an essential cell cycle gene in Saccharomyces cerevisiae, encodes a protein kinase domain.
1991,
Pubmed
Seufert,
Role of a ubiquitin-conjugating enzyme in degradation of S- and M-phase cyclins.
1995,
Pubmed
,
Xenbase
Seufert,
Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins.
1990,
Pubmed
Spence,
A ubiquitin mutant with specific defects in DNA repair and multiubiquitination.
1995,
Pubmed
Sudakin,
The cyclosome, a large complex containing cyclin-selective ubiquitin ligase activity, targets cyclins for destruction at the end of mitosis.
1995,
Pubmed
Surana,
Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast.
1993,
Pubmed
Tugendreich,
CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition.
1995,
Pubmed
Tyers,
Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins.
1993,
Pubmed
Tzamarias,
Distinct TPR motifs of Cyc8 are involved in recruiting the Cyc8-Tup1 corepressor complex to differentially regulated promoters.
1995,
Pubmed
Whitfield,
The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transition.
1990,
Pubmed
Xiao,
CSE1 and CSE2, two new genes required for accurate mitotic chromosome segregation in Saccharomyces cerevisiae.
1993,
Pubmed
