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Pflugers Arch
1996 May 01;4321:134-43. doi: 10.1007/s004240050115.
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Functional characterization of two 5-HT3 receptor splice variants isolated from a mouse hippocampal cell line.
Glitsch M
,
Wischmeyer E
,
Karschin A
.
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Two splice variants of the ligand-gated 5-hydroxytryptamine or serotonin 5-HT3 receptor that differ in a six-amino-acid deletion were cloned by polymerase chain reaction from the hippocampus x neuroblastoma cell line HN9.10e. When expressed in Xenopus oocytes, both variants individually formed 5-HT3 receptors that revealed no significant differences in current responses to the agonists 5-HT and 1-phenylbiguanide and block by the specific antagonist LY-278, 584-maleate. For both receptors, the monovalent cations Na+, K+, Rb+ and Li+ showed the same relative permeability; NH4(+)permeated approximately 2.7 times better than Na+, and Tris+ was only poorly permeable. In contrast to other reports, the receptors were completely and reversibly blocked by extracellular Cs+ in both oocytes and native HN9.10 cells. Moreover, Ca2+ was not permeant and exhibited a concentration-dependent decrease (0.9-18 mM) of the 5-HT-induced currents without affecting the inward rectification of the current/voltage relation. The two receptors were reversibly inhibited by nanomolar concentrations of the specific inhibitor of protein kinase C (PKC) bisindolylmaleimide, but not by the equipotent and less specific inhibitor staurosporine. A regulatory effect on both 5-HT3 receptor subunits by PKC-mediated protein phosphorylation might be possible, however, a functional role of the two splice variants present in one cell remains to be determined.
Derkach,
5-HT3 receptors are membrane ion channels.
1989, Pubmed
Derkach,
5-HT3 receptors are membrane ion channels.
1989,
Pubmed
Downie,
Pharmacological characterization of the apparent splice variants of the murine 5-HT3 R-A subunit expressed in Xenopus laevis oocytes.
1994,
Pubmed
,
Xenbase
Eckstein,
Nucleoside phosphorothioates.
1985,
Pubmed
Eiselé,
Chimaeric nicotinic-serotonergic receptor combines distinct ligand binding and channel specificities.
1993,
Pubmed
,
Xenbase
Gilon,
Activation of 5-HT3 receptors expressed in Xenopus oocytes does not increase cytoplasmic Ca2+ levels.
1995,
Pubmed
,
Xenbase
Goldin,
Maintenance of Xenopus laevis and oocyte injection.
1992,
Pubmed
,
Xenbase
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Hope,
Cloning and functional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit.
1993,
Pubmed
,
Xenbase
Hoyer,
International Union of Pharmacology classification of receptors for 5-hydroxytryptamine (Serotonin).
1994,
Pubmed
Hussy,
Functional properties of a cloned 5-hydroxytryptamine ionotropic receptor subunit: comparison with native mouse receptors.
1994,
Pubmed
Imoto,
Rings of negatively charged amino acids determine the acetylcholine receptor channel conductance.
1988,
Pubmed
,
Xenbase
Lee,
Neuronal properties and trophic activities of immortalized hippocampal cells from embryonic and young adult mice.
1990,
Pubmed
Maricq,
Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel.
1991,
Pubmed
,
Xenbase
Miquel,
Involvement of tryptophan residue(s) in the specific binding of agonists/antagonists to 5-HT3 receptors in NG108-15 clonal cells.
1991,
Pubmed
Miquel,
Developmental changes in the differential expression of two serotonin 5-HT3 receptor splice variants in the rat.
1995,
Pubmed
Pearson,
Protein kinase phosphorylation site sequences and consensus specificity motifs: tabulations.
1991,
Pubmed
Peters,
Divalent cations modulate 5-HT3 receptor-induced currents in N1E-115 neuroblastoma cells.
1988,
Pubmed
Robertson,
Properties of 5-hydroxytryptamine3 receptor-gated currents in adult rat dorsal root ganglion neurones.
1991,
Pubmed
Swope,
Phosphorylation of ligand-gated ion channels: a possible mode of synaptic plasticity.
1992,
Pubmed
Toullec,
The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C.
1991,
Pubmed
Uetz,
Organisation of the murine 5-HT3 receptor gene and assignment to human chromosome 11.
1994,
Pubmed
,
Xenbase
Van Hooft,
Phosphorylation controls conductance of 5-HT3 receptor ligand-gated ion channels.
1995,
Pubmed
Werner,
Organization of the mouse 5-HT3 receptor gene and functional expression of two splice variants.
1994,
Pubmed
Yakel,
Activation and desensitization of the 5-HT3 receptor in a rat glioma x mouse neuroblastoma hybrid cell.
1991,
Pubmed
Yakel,
The selectivity of the channel coupled to the 5-HT3 receptor.
1990,
Pubmed
Yakel,
5-HT3 receptors mediate rapid responses in cultured hippocampus and a clonal cell line.
1988,
Pubmed
Yang,
Ion permeation through 5-hydroxytryptamine-gated channels in neuroblastoma N18 cells.
1990,
Pubmed
Yang,
5-HT3 receptor channels in dissociated rat superior cervical ganglion neurons.
1992,
Pubmed
