Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1995 Oct 16;1420:5027-36. doi: 10.1002/j.1460-2075.1995.tb00185.x.
Show Gene links
Show Anatomy links
MAT1 ('menage à trois') a new RING finger protein subunit stabilizing cyclin H-cdk7 complexes in starfish and Xenopus CAK.
Devault A
,
Martinez AM
,
Fesquet D
,
Labbé JC
,
Morin N
,
Tassan JP
,
Nigg EA
,
Cavadore JC
,
Dorée M
.
???displayArticle.abstract???
The kinase responsible for Thr161-Thr160 phosphorylation and activation of cdc2/cdk2 (CAK:cdk-activating kinase) has been shown previously to comprise at least two subunits, cdk7 and cyclin H. An additional protein co-purified with CAK in starfish oocytes, but its sequencing did not reveal any similarity with any known protein. In the present work, a cDNA encoding this protein is cloned and sequenced in both starfish and Xenopus oocytes. It is shown to encode a new member of the RING finger family of proteins with a characteristic C3HC4 motif located in the N-terminal domain. We demonstrate that the RING finger protein (MAT1: 'menage à trois') is a new subunit of CAK in both vertebrate and invertebrates. However, CAK may also exist in oocytes as heterodimeric complexes between cyclin H and cdk7 only. Stable heterotrimeric CAK complexes were generated in reticulocyte lysates programmed with mRNAs encoding Xenopus cdk7, cyclin H and MAT1. In contrast, no heterodimeric cyclin H-cdk7 complex could be immunoprecipitated from reticulocyte lysates programmed with cdk7 and cyclin H mRNAs only. Stabilization of CAK complexes by MAT1 does not involve phosphorylation of Thr176, as the Thr176-->Ala mutant of Xenopus cdk7 could engage as efficiently as wild-type cdk7 in ternary complexes. Even though starfish MAT1 is almost identical to Xenopus MAT1 in the RING finger domain, the starfish subunit could not replace the Xenopus subunit and stabilize cyclin H-cdk7 in reticulocyte lysate, suggesting that the MAT1 subunit does not (or not only) interact with cyclin H-cdk7 through the RING finger domain.
Darbon,
Cloning, expression and subcellular localization of the human homolog of p40MO15 catalytic subunit of cdk-activating kinase.
1994, Pubmed,
Xenbase
Darbon,
Cloning, expression and subcellular localization of the human homolog of p40MO15 catalytic subunit of cdk-activating kinase.
1994,
Pubmed
,
Xenbase
De Bondt,
Crystal structure of cyclin-dependent kinase 2.
1993,
Pubmed
Desai,
Effects of phosphorylation by CAK on cyclin binding by CDC2 and CDK2.
1995,
Pubmed
Dorée,
The cyclin-dependent protein kinases and the control of cell division.
1994,
Pubmed
Ducommun,
cdc2 phosphorylation is required for its interaction with cyclin.
1991,
Pubmed
,
Xenbase
Endicott,
The cell cycle and suc1: from structure to function?
1995,
Pubmed
Feaver,
Relationship of CDK-activating kinase and RNA polymerase II CTD kinase TFIIH/TFIIK.
1994,
Pubmed
Fesquet,
The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues.
1993,
Pubmed
,
Xenbase
Fisher,
A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase.
1994,
Pubmed
Freemont,
The RING finger. A novel protein sequence motif related to the zinc finger.
1993,
Pubmed
Fyrberg,
Genes encoding actin-related proteins of Drosophila melanogaster.
1994,
Pubmed
Gould,
Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function.
1991,
Pubmed
Hadwiger,
The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex.
1989,
Pubmed
Hanks,
Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification.
1995,
Pubmed
Hanson,
New genes in the class II region of the human major histocompatibility complex.
1991,
Pubmed
Hunt,
Cyclins and their partners: from a simple idea to complicated reality.
1991,
Pubmed
Jessus,
Oscillation of MPF is accompanied by periodic association between cdc25 and cdc2-cyclin B.
1992,
Pubmed
,
Xenbase
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Labbé,
p40MO15 associates with a p36 subunit and requires both nuclear translocation and Thr176 phosphorylation to generate cdk-activating kinase activity in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Lees,
Sequences within the conserved cyclin box of human cyclin A are sufficient for binding to and activation of cdc2 kinase.
1993,
Pubmed
,
Xenbase
Lovering,
Identification and preliminary characterization of a protein motif related to the zinc finger.
1993,
Pubmed
Mäkelä,
A cyclin associated with the CDK-activating kinase MO15.
1994,
Pubmed
,
Xenbase
Matsuoka,
Activation of cyclin-dependent kinase 4 (cdk4) by mouse MO15-associated kinase.
1994,
Pubmed
,
Xenbase
Michaille,
Isolation and characterization of a cDNA encoding a chicken actin-like protein.
1995,
Pubmed
Morgan,
Principles of CDK regulation.
1995,
Pubmed
Mueller,
Cell cycle regulation of a Xenopus Wee1-like kinase.
1995,
Pubmed
,
Xenbase
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Norbury,
Animal cell cycles and their control.
1992,
Pubmed
Norbury,
Regulatory phosphorylation of the p34cdc2 protein kinase in vertebrates.
1991,
Pubmed
,
Xenbase
Parge,
Human CksHs2 atomic structure: a role for its hexameric assembly in cell cycle control.
1993,
Pubmed
Poon,
The cdc2-related protein p40MO15 is the catalytic subunit of a protein kinase that can activate p33cdk2 and p34cdc2.
1993,
Pubmed
,
Xenbase
Reddy,
The cloning and characterization of a maternally expressed novel zinc finger nuclear phosphoprotein (xnf7) in Xenopus laevis.
1991,
Pubmed
,
Xenbase
Richardson,
Human cDNAs encoding homologs of the small p34Cdc28/Cdc2-associated protein of Saccharomyces cerevisiae and Schizosaccharomyces pombe.
1990,
Pubmed
Roy,
The MO15 cell cycle kinase is associated with the TFIIH transcription-DNA repair factor.
1994,
Pubmed
Schwob,
New yeast actin-like gene required late in the cell cycle.
1992,
Pubmed
Serizawa,
Association of Cdk-activating kinase subunits with transcription factor TFIIH.
1995,
Pubmed
Sherr,
Mammalian G1 cyclins.
1993,
Pubmed
Shuttleworth,
p40MO15, a cdc2-related protein kinase involved in negative regulation of meiotic maturation of Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Solomon,
CAK, the p34cdc2 activating kinase, contains a protein identical or closely related to p40MO15.
1993,
Pubmed
,
Xenbase
Solomon,
The function(s) of CAK, the p34cdc2-activating kinase.
1994,
Pubmed
Tagawa,
Expression of novel DNA-binding protein with zinc finger structure in various tumor cells.
1990,
Pubmed
Tassan,
Cell cycle analysis of the activity, subcellular localization, and subunit composition of human CAK (CDK-activating kinase).
1994,
Pubmed
,
Xenbase
Zhang,
Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes.
1993,
Pubmed