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DF 31, a sperm decondensation factor from Drosophila melanogaster: purification and characterization.
Crevel G
,
Cotterill S
.
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We have purified to homogeneity a Drosophila protein which is able to decondense Xenopus sperm chromatin. This protein, which we have called DF 31, is a heat-stable phosphoprotein which displays a molecular weight of 31 kDa on SDS-PAGE, but which has an apparent molecular weight of > 200 kDa on gel filtration. We show that DF 31 decondenses sperm DNA by displacement of sperm-specific proteins. In addition to its sperm decondensation activity, DF 31 is also able to facilitate nucleosome loading on both decondensed sperm DNA and on naked DNA template. The reaction as catalysed by DF 31 is quite efficient; however, the nucleosomes appear to be loaded randomly onto the DNA, not in regular arrays. Although the mechanism by which DF 31 aids nucleosome loading is not yet clear, it most probably occurs through binding of DF 31 to core histones.
Allis,
Mass isolation of pole cells from Drosophila melanogaster.
1977, Pubmed
Allis,
Mass isolation of pole cells from Drosophila melanogaster.
1977,
Pubmed
Berrios,
Nuclear formation in a Drosophila cell-free system.
1990,
Pubmed
,
Xenbase
Bonne-Andrea,
Rat liver HMG1: a physiological nucleosome assembly factor.
1984,
Pubmed
Chen,
A histone-binding protein, nucleoplasmin, stimulates transcription factor binding to nucleosomes and factor-induced nucleosome disassembly.
1994,
Pubmed
Cotten,
Purification of a novel, nucleoplasmin-like protein from somatic nuclei.
1987,
Pubmed
,
Xenbase
Cotten,
Massive phosphorylation distinguishes Xenopus laevis nucleoplasmin isolated from oocytes or unfertilized eggs.
1986,
Pubmed
,
Xenbase
Crevel,
DNA replication in cell-free extracts from Drosophila melanogaster.
1991,
Pubmed
,
Xenbase
de Cicco,
Localization of a cis-acting element responsible for the developmentally regulated amplification of Drosophila chorion genes.
1984,
Pubmed
Dilworth,
Two complexes that contain histones are required for nucleosome assembly in vitro: role of nucleoplasmin and N1 in Xenopus egg extracts.
1987,
Pubmed
,
Xenbase
Edgar,
Distinct molecular mechanism regulate cell cycle timing at successive stages of Drosophila embryogenesis.
1994,
Pubmed
Ellis,
Molecular chaperones.
1991,
Pubmed
Gruss,
Disruption of the nucleosomes at the replication fork.
1993,
Pubmed
,
Xenbase
Ishimi,
Identification and molecular cloning of yeast homolog of nucleosome assembly protein I which facilitates nucleosome assembly in vitro.
1991,
Pubmed
,
Xenbase
Kawasaki,
Chromatin decondensation in Drosophila embryo extracts.
1994,
Pubmed
,
Xenbase
Kleinschmidt,
Soluble acidic complexes containing histones H3 and H4 in nuclei of Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Kleinschmidt,
Nucleosome assembly in vitro: separate histone transfer and synergistic interaction of native histone complexes purified from nuclei of Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Laskey,
Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA.
1978,
Pubmed
,
Xenbase
Lässle,
Comparison of replicative and non-replicative chromatin assembly pathways in HeLa cell extracts.
1992,
Pubmed
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Mills,
An acidic protein which assembles nucleosomes in vitro is the most abundant protein in Xenopus oocyte nuclei.
1980,
Pubmed
,
Xenbase
Ohsumi,
Characterization of the ooplasmic factor inducing decondensation of and protamine removal from toad sperm nuclei: involvement of nucleoplasmin.
1991,
Pubmed
,
Xenbase
Philpott,
Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin.
1991,
Pubmed
,
Xenbase
Smith,
Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro.
1991,
Pubmed
Travers,
DNA chaperones: a solution to a persistence problem?
1994,
Pubmed
Ulitzur,
Nuclear envelope assembly around sperm chromatin in cell-free preparations from Drosophila embryos.
1989,
Pubmed
Zweidler,
Resolution of histones by polyacrylamide gel electrophoresis in presence of nonionic detergents.
1978,
Pubmed
