Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Human TFIIIA alone is sufficient to prevent nucleosomal repression of a homologous 5S gene.
Stünkel W
,
Kober I
,
Kauer M
,
Taimor G
,
Seifart KH
.
???displayArticle.abstract???
Plasmid DNA harbouring the human 5S rRNA gene was assembled into nucleosomes using either Xenopus S150 extracts or purified core histones in the presence of pectin. In both cases reconstitution of nucleosomes led to a complete repression of transcription. This repression could be efficiently counteracted by preincubating the template DNA with highly purified hTFIIIA which allowed the protein to bind to the ICR of the 5S gene. By using an efficient and well-defined in vitro reconstitution system based on isolated core histones in the presence of pectin, which is devoid of endogenous transcription factors, we demonstrate here for the first time that human TFIIIA alone is sufficient to prevent nucleosomal repression of h5S gene transcription and that additional pol III transcription factors are not required to achieve this effect. Additionally, we investigated the binding of hTFIIIA to a mononucleosome reconstituted on the human 5S gene. DNAse I footprinting experiments reveal that the entire ICR of the human 5S gene is covered by the nucleosome, thereby precluding the subsequent binding of human TFIIIA to the promoter of the 5S gene.
Almouzni,
Chromatin assembly on replicating DNA in vitro.
1990, Pubmed,
Xenbase
Almouzni,
Chromatin assembly on replicating DNA in vitro.
1990,
Pubmed
,
Xenbase
Almouzni,
Competition between transcription complex assembly and chromatin assembly on replicating DNA.
1990,
Pubmed
,
Xenbase
Almouzni,
Transcription complex disruption caused by a transition in chromatin structure.
1991,
Pubmed
,
Xenbase
Archambault,
The deduced sequence of the transcription factor TFIIIA from Saccharomyces cerevisiae reveals extensive divergence from Xenopus TFIIIA.
1992,
Pubmed
,
Xenbase
Barton,
The erythroid protein cGATA-1 functions with a stage-specific factor to activate transcription of chromatin-assembled beta-globin genes.
1993,
Pubmed
Bauer,
Nucleosome structural changes due to acetylation.
1994,
Pubmed
,
Xenbase
Bieker,
Physical properties and DNA-binding stoichiometry of a 5 S gene-specific transcription factor.
1984,
Pubmed
,
Xenbase
Bogenhagen,
Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state.
1982,
Pubmed
,
Xenbase
Chen,
Rapid and reversible changes in nucleosome structure accompany the activation, repression, and superinduction of murine fibroblast protooncogenes c-fos and c-myc.
1987,
Pubmed
Chen,
A histone-binding protein, nucleoplasmin, stimulates transcription factor binding to nucleosomes and factor-induced nucleosome disassembly.
1994,
Pubmed
Csordas,
On the biological role of histone acetylation.
1990,
Pubmed
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Felsenfeld,
Chromatin as an essential part of the transcriptional mechanism.
1992,
Pubmed
Felts,
Transcription factor requirements for in vitro formation of transcriptionally competent 5S rRNA gene chromatin.
1990,
Pubmed
Geiduschek,
Transcription by RNA polymerase III.
1988,
Pubmed
Ginsberg,
Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.
1984,
Pubmed
,
Xenbase
Gottesfeld,
Assembly of transcriptionally active 5S RNA gene chromatin in vitro.
1982,
Pubmed
,
Xenbase
Gottesfeld,
DNA sequence-directed nucleosome reconstitution on 5S RNA genes of Xenopus laevis.
1987,
Pubmed
,
Xenbase
Grunstein,
Histone function in transcription.
1990,
Pubmed
Hayes,
Histone contributions to the structure of DNA in the nucleosome.
1991,
Pubmed
,
Xenbase
Hayes,
The structure of DNA in a nucleosome.
1990,
Pubmed
,
Xenbase
Hayes,
Histones H2A/H2B inhibit the interaction of transcription factor IIIA with the Xenopus borealis somatic 5S RNA gene in a nucleosome.
1992,
Pubmed
,
Xenbase
Hebbes,
A direct link between core histone acetylation and transcriptionally active chromatin.
1988,
Pubmed
Imbalzano,
Facilitated binding of TATA-binding protein to nucleosomal DNA.
1994,
Pubmed
Jahn,
Transcription complexes for various class III genes differ in parameters of formation and stability towards salt.
1987,
Pubmed
Knezetic,
The presence of nucleosomes on a DNA template prevents initiation by RNA polymerase II in vitro.
1986,
Pubmed
,
Xenbase
Knezetic,
Assembly of RNA polymerase II preinitiation complexes before assembly of nucleosomes allows efficient initiation of transcription on nucleosomal templates.
1988,
Pubmed
Kwon,
Nucleosome disruption and enhancement of activator binding by a human SW1/SNF complex.
1994,
Pubmed
,
Xenbase
Laskey,
Chromatin assembly and transcription in eggs and oocytes of Xenopus laevis.
1978,
Pubmed
,
Xenbase
Laybourn,
Role of nucleosomal cores and histone H1 in regulation of transcription by RNA polymerase II.
1991,
Pubmed
Lee,
A positive role for histone acetylation in transcription factor access to nucleosomal DNA.
1993,
Pubmed
,
Xenbase
Li,
Effects of histone acetylation, ubiquitination and variants on nucleosome stability.
1993,
Pubmed
Lorch,
Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones.
1987,
Pubmed
Matsui,
Transcription of adenovirus 2 major late and peptide IX genes under conditions of in vitro nucleosome assembly.
1987,
Pubmed
Meisterernst,
Recombinant yeast TFIID, a general transcription factor, mediates activation by the gene-specific factor USF in a chromatin assembly assay.
1990,
Pubmed
,
Xenbase
Moorefield,
Purification and characterization of human transcription factor IIIA.
1994,
Pubmed
,
Xenbase
Morse,
Nucleosomes inhibit both transcriptional initiation and elongation by RNA polymerase III in vitro.
1989,
Pubmed
,
Xenbase
Oliva,
Histone hyperacetylation can induce unfolding of the nucleosome core particle.
1990,
Pubmed
Peck,
Energetics of B-to-Z transition in DNA.
1983,
Pubmed
Ramsay,
A 145-base pair DNA sequence that positions itself precisely and asymmetrically on the nucleosome core.
1984,
Pubmed
Rhodes,
Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA.
1985,
Pubmed
,
Xenbase
Seifart,
Purification of human transcription factor IIIA and its interaction with a chemically synthesized gene encoding human 5 S rRNA.
1989,
Pubmed
,
Xenbase
Shimamura,
Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Simpson,
Structural features of a phased nucleosome core particle.
1983,
Pubmed
Simpson,
Structure of the chromatosome, a chromatin particle containing 160 base pairs of DNA and all the histones.
1978,
Pubmed
Smith,
Two-stage maturation process for newly replicated chromatin.
1984,
Pubmed
Sobolewski,
A novel nucleosome assembly procedure (with a little help from pectin).
1993,
Pubmed
Sørensen,
Nucleotide sequence of a human 5S rRNA gene.
1990,
Pubmed
,
Xenbase
Stein,
Reconstitution of chromatin from purified components.
1989,
Pubmed
Svaren,
The structure and assembly of active chromatin.
1990,
Pubmed
Tremethick,
The transcription complex of the 5 S RNA gene, but not transcription factor IIIA alone, prevents nucleosomal repression of transcription.
1990,
Pubmed
,
Xenbase
Waldschmidt,
Physical and immunological characterization of human transcription factor IIIA.
1990,
Pubmed
,
Xenbase
Weisbrod,
Active chromatin of oocytes injected with somatic cell nuclei or cloned DNA.
1982,
Pubmed
,
Xenbase
Wolffe,
DNA replication in vitro erases a Xenopus 5S RNA gene transcription complex.
1986,
Pubmed
,
Xenbase
Wolffe,
Developmental regulation of two 5S ribosomal RNA genes.
1988,
Pubmed
,
Xenbase
Workman,
An upstream transcription factor, USF (MLTF), facilitates the formation of preinitiation complexes during in vitro chromatin assembly.
1990,
Pubmed
,
Xenbase
Workman,
Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
1987,
Pubmed
,
Xenbase
Woychik,
Genes encoding transcription factor IIIA and the RNA polymerase common subunit RPB6 are divergently transcribed in Saccharomyces cerevisiae.
1992,
Pubmed
,
Xenbase
