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XB-ART-20678
Nature 1994 Oct 13;3716498:612-4. doi: 10.1038/371612a0.
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Activation of Raf-1 by 14-3-3 proteins.

Fantl WJ , Muslin AJ , Kikuchi A , Martin JA , MacNicol AM , Gross RW , Williams LT .


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The protein Raf-1, a key mediator of mitogenesis and differentiation, associates with p21ras (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood. Using the yeast two-hybrid system, we identified two structurally related proteins that interact with the aminoterminal region of Raf-1. These proteins, 14-3-3 zeta (PLA2) and 14-3-3 beta (HS1), are members of the 14-3-3 family of proteins. Expression of 14-3-3 proteins in Xenopus oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.

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Species referenced: Xenopus
Genes referenced: hcls1 pla2g1b raf1 ywhab ywhaz