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XB-ART-21258
Nucleic Acids Res 1994 May 11;229:1675-80.
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The promoter of the Xwnt-5C gene contains octamer and AP-2 motifs functional in Xenopus embryos.

Kuiken GA , Bertens PJ , Peterson-Maduro J , Veenstra GJ , Koster JG , Destrée OH .


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The Xwnt-5C gene is expressed in Xenopus embryos from the early gastrula stage onwards. The transcription of Xwnt-5C is regulated differentially with respect to transcript size, timing and localization. To gain insight into the generation of the Xwnt-5C expression pattern, we started to analyze the transcriptional regulation of this gene. We isolated Xwnt-5C genomic DNA sequences. By microinjection of chimaeric reporter constructs into Xenopus embryos we demonstrate that the upstream region contains a promoter functional in vivo. Of the several putative binding sites for trans-acting factors, present in a minimal promoter fragment, some have been studied in more detail. Mutations in an octamer motif and in an AP-2 consensus sequence interfere with the activity of the Xwnt-5C minimal promoter. In vitro binding assays with extracts from gastrula stage Xenopus embryos show that the octamer motif of the Xwnt-5C promoter can bind several Octamer binding factors, one of which is Oct1.

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Species referenced: Xenopus
Genes referenced: pou2f1 tfap2a

References [+] :
Christian, Isolation of cDNAs partially encoding four Xenopus Wnt-1/int-1-related proteins and characterization of their transient expression during embryonic development. 1991, Pubmed, Xenbase