Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Clustering of the acetylcholine receptor by the 43-kD protein: involvement of the zinc finger domain.
Scotland PB
,
Colledge M
,
Melnikova I
,
Dai Z
,
Froehner SC
.
???displayArticle.abstract???
A postsynaptic membrane-associated protein of M(r) 43,000 (43-kD protein) is involved in clustering of the nicotinic acetylcholine receptor (AChR) at the neuromuscular junction. Previous studies have shown that recombinant mouse 43-kD protein forms membrane-associated clusters when expressed in Xenopus oocytes. Coexpression with the AChR results in colocalization of the receptor with the 43-kD protein clusters (Froehner, S. C., C. W. Luetje, P. B. Scotland, and J. Patrick, 1990. Neuron. 5:403-410). To understand the mechanism of this clustering, we have studied the role of the carboxy-terminal region of the 43-kD protein. The amino acid sequence of this region predicts two tandem zinc finger structures followed by a serine phosphorylation site. Both Torpedo 43-kD protein and the carboxy-terminal region of the mouse 43-kD protein bind radioisotopic zinc. Mutation of two histidine residues in this predicted domain greatly attenuates zinc binding, lending support to the proposal that this region forms zinc fingers. When expressed in oocytes, the ability of this mutant 43-kD protein to form clusters is greatly reduced. Its ability to interact with AChR, however, is retained. In contrast, a mutation that eliminates the potential serine phosphorylation site has no effect on clustering of the 43-kD protein or on interaction with the AChR. These findings suggest that protein interactions via the zinc finger domain of the 43-kD protein may be important for AChR clustering at the synapse.
Ahmed,
The cysteine-rich domain of human proteins, neuronal chimaerin, protein kinase C and diacylglycerol kinase binds zinc. Evidence for the involvement of a zinc-dependent structure in phorbol ester binding.
1991, Pubmed
Ahmed,
The cysteine-rich domain of human proteins, neuronal chimaerin, protein kinase C and diacylglycerol kinase binds zinc. Evidence for the involvement of a zinc-dependent structure in phorbol ester binding.
1991,
Pubmed
Barrantes,
Peptide extraction by alkaline treatment is accompanied by rearrangement of the membrane-bound acetylcholine receptor from Torpedo marmorata.
1980,
Pubmed
Berg,
Zinc fingers and other metal-binding domains. Elements for interactions between macromolecules.
1990,
Pubmed
Bloch,
The relationship of the postsynaptic 43K protein to acetylcholine receptors in receptor clusters isolated from cultured rat myotubes.
1987,
Pubmed
Brennan,
Functional properties of acetylcholine receptors coexpressed with the 43K protein in heterologous cell systems.
1992,
Pubmed
,
Xenbase
Bridgman,
Visualization of the cytoplasmic surface of Torpedo postsynaptic membranes by freeze-etch and immunoelectron microscopy.
1987,
Pubmed
Burden,
Crosslinking of proteins in acetylcholine receptor-rich membranes: association between the beta-subunit and the 43 kd subsynaptic protein.
1983,
Pubmed
Butler,
Association of the Mr 58,000 postsynaptic protein of electric tissue with Torpedo dystrophin and the Mr 87,000 postsynaptic protein.
1992,
Pubmed
Campanelli,
Agrin mediates cell contact-induced acetylcholine receptor clustering.
1991,
Pubmed
Carr,
Myristic acid is the NH2-terminal blocking group of the 43-kDa protein of Torpedo nicotinic post-synaptic membranes.
1989,
Pubmed
Ferns,
How many agrins does it take to make a synapse?
1992,
Pubmed
Fertuck,
Localization of acetylcholine receptor by 125I-labeled alpha-bungarotoxin binding at mouse motor endplates.
1974,
Pubmed
Flucher,
Distribution of Na+ channels and ankyrin in neuromuscular junctions is complementary to that of acetylcholine receptors and the 43 kd protein.
1989,
Pubmed
Freemont,
A novel cysteine-rich sequence motif.
1991,
Pubmed
Froehner,
Expression of RNA transcripts for the postsynaptic 43 kDa protein in innervated and denervated rat skeletal muscle.
1989,
Pubmed
,
Xenbase
Froehner,
Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor.
1983,
Pubmed
Froehner,
The postsynaptic 43K protein clusters muscle nicotinic acetylcholine receptors in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Froehner,
The submembrane machinery for nicotinic acetylcholine receptor clustering.
1991,
Pubmed
Froehner,
Regulation of ion channel distribution at synapses.
1993,
Pubmed
Goddard,
Characterization of a zinc finger gene disrupted by the t(15;17) in acute promyelocytic leukemia.
1991,
Pubmed
Gordon,
A muscle cell variant defective in glycosaminoglycan biosynthesis forms nerve-induced but not spontaneous clusters of the acetylcholine receptor and the 43 kDa protein.
1993,
Pubmed
Hall,
Synaptic structure and development: the neuromuscular junction.
1993,
Pubmed
Hill,
Serine-specific phosphorylation of nicotinic receptor associated 43K protein.
1991,
Pubmed
Hill,
Nicotinic receptor-associated 43K protein and progressive stabilization of the postsynaptic membrane.
1992,
Pubmed
,
Xenbase
Ho,
Site-directed mutagenesis by overlap extension using the polymerase chain reaction.
1989,
Pubmed
Kakizuka,
Chromosomal translocation t(15;17) in human acute promyelocytic leukemia fuses RAR alpha with a novel putative transcription factor, PML.
1991,
Pubmed
Kordeli,
Asynchronous assembly of the acetylcholine receptor and of the 43-kD nu1 protein in the postsynaptic membrane of developing Torpedo marmorata electrocyte.
1989,
Pubmed
LaRochelle,
Developmental expression of the 43K and 58K postsynaptic membrane proteins and nicotinic acetylcholine receptors in Torpedo electrocytes.
1990,
Pubmed
LaRochelle,
Comparison of the postsynaptic 43-kDa protein from muscle cells that differ in acetylcholine receptor clustering activity.
1987,
Pubmed
LaRochelle,
Determination of the tissue distributions and relative concentrations of the postsynaptic 43-kDa protein and the acetylcholine receptor in Torpedo.
1986,
Pubmed
LaRochelle,
Clusters of 43-kDa protein are absent from genetic variants of C2 muscle cells with reduced acetylcholine receptor expression.
1989,
Pubmed
LaRochelle,
Immunochemical demonstration that amino acids 360-377 of the acetylcholine receptor gamma-subunit are cytoplasmic.
1985,
Pubmed
Lo,
Rotational mobility of the membrane-bound acetylcholine receptor of Torpedo electric organ measured by phosphorescence depolarisation.
1980,
Pubmed
McMahan,
The agrin hypothesis.
1990,
Pubmed
Mitra,
Three-dimensional structure of the nicotinic acetylcholine receptor and location of the major associated 43-kD cytoskeletal protein, determined at 22 A by low dose electron microscopy and x-ray diffraction to 12.5 A.
1989,
Pubmed
Musil,
Acetylcholine receptor-associated 43K protein contains covalently bound myristate.
1988,
Pubmed
Nastuk,
Agrin and the molecular choreography of synapse formation.
1993,
Pubmed
Neubig,
Acetylcholine and local anesthetic binding to Torpedo nicotinic postsynaptic membranes after removal of nonreceptor peptides.
1979,
Pubmed
Noakes,
43K protein and acetylcholine receptors colocalize during the initial stages of neuromuscular synapse formation in vivo.
1993,
Pubmed
Pan,
GAL4 transcription factor is not a "zinc finger" but forms a Zn(II)2Cys6 binuclear cluster.
1990,
Pubmed
Peng,
Association of the postsynaptic 43K protein with newly formed acetylcholine receptor clusters in cultured muscle cells.
1985,
Pubmed
,
Xenbase
Phillips,
ACh receptor-rich membrane domains organized in fibroblasts by recombinant 43-kildalton protein.
1991,
Pubmed
Phillips,
Mutagenesis of the 43-kD postsynaptic protein defines domains involved in plasma membrane targeting and AChR clustering.
1991,
Pubmed
Porter,
Interaction of the 43K protein with components of Torpedo postsynaptic membranes.
1985,
Pubmed
Pradier,
Use of chemical modifications and site-directed mutagenesis to probe the functional role of thiol groups on the gamma subunit of Torpedo californica acetylcholine receptor.
1989,
Pubmed
,
Xenbase
Quest,
The regulatory domain of protein kinase C coordinates four atoms of zinc.
1992,
Pubmed
Reddy,
A novel zinc finger coiled-coil domain in a family of nuclear proteins.
1992,
Pubmed
Ross,
Induction of phosphorylation and cell surface redistribution of acetylcholine receptors by phorbol ester and carbamylcholine in cultured chick muscle cells.
1988,
Pubmed
Rousselet,
The rotational diffusion of the acetylcholine receptor in Torpeda marmorata membrane fragments studied with a spin-labelled alpha-toxin: importance of the 43 000 protein(s).
1982,
Pubmed
Sadler,
Zyxin and cCRP: two interactive LIM domain proteins associated with the cytoskeleton.
1992,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schwabe,
Beyond zinc fingers: steroid hormone receptors have a novel structural motif for DNA recognition.
1991,
Pubmed
Sealock,
Ultrastructural localization of the Mr 43,000 protein and the acetylcholine receptor in Torpedo postsynaptic membranes using monoclonal antibodies.
1984,
Pubmed
Toyoshima,
Ion channel of acetylcholine receptor reconstructed from images of postsynaptic membranes.
1988,
Pubmed
Treich,
Zinc-binding subunits of yeast RNA polymerases.
1991,
Pubmed
Vallee,
Zinc fingers, zinc clusters, and zinc twists in DNA-binding protein domains.
1991,
Pubmed
Vallee,
The metallobiochemistry of zinc enzymes.
1984,
Pubmed
Vallee,
Cocatalytic zinc motifs in enzyme catalysis.
1993,
Pubmed
Wallace,
Regulation of agrin-induced acetylcholine receptor aggregation by Ca++ and phorbol ester.
1988,
Pubmed
Yoshihara,
Increased expression of the 43-kD protein disrupts acetylcholine receptor clustering in myotubes.
1993,
Pubmed
