Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Interaction of proteins with the mRNA for ribosomal protein L1 in Xenopus: structural characterization of in vivo complexes and identification of proteins that bind in vitro to its 5'UTR.
Cardinali B
,
Di Cristina M
,
Pierandrei-Amaldi P
.
???displayArticle.abstract???
Xenopus r-protein mRNAs are known to be coordinately regulated at the translational level. To find out if RNA/protein interactions are involved in this control mechanism, we have characterized the particles containing the translationally repressed rp-mRNA and we have investigated the proteins that specifically bind to this type of mRNA. By sedimentation analysis and isopycnic centrifugation we have found that the repressed rp-mRNAs are assembled in slow sedimenting complexes where the RNA is prevalent over the protein mass (2.3 to 1). This composition is maintained also after in vitro reconstitution of the particle. We carried out also a detailed analysis of in vitro RNA/protein complex formation by focusing our attention on the 5'UTR, very similar in different rp-mRNAs and important in the translational regulation. We describe specific interactions of L1 mRNA with four proteins. The binding site of two of them, 57 kD and 47 kD, is in the typical pyrimidine sequence at the 5' end and is position dependent. Proteins of the same size interact also with the analogous region of r-protein S1 and L14 mRNA, not with unrelated RNAs. Binding of two other proteins, 31 kD and 24 kD, in the downstream region of the 5'UTR was also observed. The most evident 57 kD protein has been partially purified. Although the binding of these proteins to the r-protein mRNA 5'UTR is specific, their involvement in the translation regulation remains to be proved.
Agrawal,
Transcriptional and translational regulation of ribosomal protein formation during mouse myoblast differentiation.
1987, Pubmed
Agrawal,
Transcriptional and translational regulation of ribosomal protein formation during mouse myoblast differentiation.
1987,
Pubmed
Al-Atia,
Translational regulation of mRNAs for ribosomal proteins during early Drosophila development.
1985,
Pubmed
Amaldi,
Translational regulation of the expression of ribosomal protein genes in Xenopus laevis.
1990,
Pubmed
,
Xenbase
Amaldi,
Expression of ribosomal protein genes and regulation of ribosome biosynthesis in Xenopus development.
1989,
Pubmed
,
Xenbase
Baum,
Coordinate expression of ribosomal protein genes during Xenopus development.
1985,
Pubmed
,
Xenbase
Cardinali,
Ribosomal protein, histone and calmodulin mRNAs are differently regulated at the translational level during oogenesis of Xenopus laevis.
1987,
Pubmed
,
Xenbase
Dandekar,
Identification of a novel iron-responsive element in murine and human erythroid delta-aminolevulinic acid synthase mRNA.
1991,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
García-Blanco,
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
1989,
Pubmed
Geyer,
Regulation of ribosomal protein mRNA content and translation in growth-stimulated mouse fibroblasts.
1982,
Pubmed
Ghetti,
hnRNP I, the polypyrimidine tract-binding protein: distinct nuclear localization and association with hnRNAs.
1992,
Pubmed
Hentze,
Oxidation-reduction and the molecular mechanism of a regulatory RNA-protein interaction.
1989,
Pubmed
Kaspar,
A regulatory cis element and a specific binding factor involved in the mitogenic control of murine ribosomal protein L32 translation.
1992,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Leibold,
Cytoplasmic protein binds in vitro to a highly conserved sequence in the 5' untranslated region of ferritin heavy- and light-subunit mRNAs.
1988,
Pubmed
Lelay-Taha,
RNA-protein organization of U1, U5 and U4-U6 small nuclear ribonucleoproteins in HeLa cells.
1986,
Pubmed
Levy,
Oligopyrimidine tract at the 5' end of mammalian ribosomal protein mRNAs is required for their translational control.
1991,
Pubmed
Loreni,
Nucleotide sequence of the L1 ribosomal protein gene of Xenopus laevis: remarkable sequence homology among introns.
1985,
Pubmed
,
Xenbase
Loreni,
Analysis of mRNAs under translational control during Xenopus embryogenesis: isolation of new ribosomal protein clones.
1992,
Pubmed
,
Xenbase
Marello,
Binding of Xenopus oocyte masking proteins to mRNA sequences.
1992,
Pubmed
,
Xenbase
Mariottini,
The 5' untranslated region of mRNA for ribosomal protein S19 is involved in its translational regulation during Xenopus development.
1990,
Pubmed
,
Xenbase
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Milligan,
Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates.
1987,
Pubmed
Murray,
Sequence analysis of cytoplasmic mRNA-binding proteins of Xenopus oocytes identifies a family of RNA-binding proteins.
1992,
Pubmed
,
Xenbase
Neupert,
A high yield affinity purification method for specific RNA-binding proteins: isolation of the iron regulatory factor from human placenta.
1990,
Pubmed
Pierandrei-Amaldi,
Experimental changes in the amount of maternally stored ribosomes affect the translation efficiency of ribosomal protein mRNA in Xenopus embryo.
1991,
Pubmed
,
Xenbase
Pierandrei-Amaldi,
Ribosomal protein production in normal and anucleolate Xenopus embryos: regulation at the posttranscriptional and translational levels.
1985,
Pubmed
,
Xenbase
Pierandrei-Amaldi,
Expression of ribosomal-protein genes in Xenopus laevis development.
1982,
Pubmed
,
Xenbase
Richter,
Reversible inhibition of translation by Xenopus oocyte-specific proteins.
,
Pubmed
,
Xenbase
Schmidt,
The induction of ribosome biosynthesis in a nonmitotic secretory tissue.
1985,
Pubmed
Steel,
Translational control of ribosomal protein synthesis during early Dictyostelium discoideum development.
1987,
Pubmed
Stutz,
Isolation and characterization of sarcomeric actin genes expressed in Xenopus laevis embryos.
1986,
Pubmed
,
Xenbase
Wagner,
Characterization of the multigene family encoding the mouse S16 ribosomal protein: strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA.
1985,
Pubmed
Wolffe,
The Y-box factors: a family of nucleic acid binding proteins conserved from Escherichia coli to man.
1992,
Pubmed
Wormington,
Developmental expression and 5S rRNA-binding activity of Xenopus laevis ribosomal protein L5.
1989,
Pubmed
,
Xenbase
