Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Isolation and characterization of a cDNA encoding Drosophila transcription factor TFIIB.
Yamashita S
,
Wada K
,
Horikoshi M
,
Gong DW
,
Kokubo T
,
Hisatake K
,
Yokotani N
,
Malik S
,
Roeder RG
,
Nakatani Y
.
???displayArticle.abstract???
A Drosophila cDNA encoding a human transcription factor TFIIB homologue was isolated by PCR methods. The deduced amino acid sequence indicates 85% sequence similarity with human TFIIB, and the corresponding cDNA product expressed in Escherichia coli is interchangeable with human TFIIB for both basal and GAL4-VP16-induced transcription. Structural motifs including the direct repeats, basic repeats, and sigma sequence similarities are well conserved among Drosophila, human, and Xenopus TFIIB. However, the N-terminal region of each direct repeat is less conserved among the three species, suggesting the presence of two structural subdomains in the direct repeat. Moreover, the amino acid changes in the N-terminal subdomain produce altered positions of the conserved amino acids between the direct repeats. An overall similarity in general structural features between TFIIB and TFIIDtau (the TATA-binding subunit of TFIID) was previously noted. However, in contrast to the sequence divergence reported for the N-terminal domains of TFIIDtau from different species, the N-terminal sequence of TFIIB was highly conserved among the species. This suggests that TFIIB has a more rigid structure, consistent with its function as a "bridging" protein between TFIID and RNA polymerase II. Further implications of the TFIIB structure are discussed.
Buratowski,
Five intermediate complexes in transcription initiation by RNA polymerase II.
1989, Pubmed
Buratowski,
Five intermediate complexes in transcription initiation by RNA polymerase II.
1989,
Pubmed
Cavallini,
Cloning of the gene encoding the yeast protein BTF1Y, which can substitute for the human TATA box-binding factor.
1989,
Pubmed
Chasman,
Activation of yeast polymerase II transcription by herpesvirus VP16 and GAL4 derivatives in vitro.
1989,
Pubmed
Ha,
Cloning of a human gene encoding the general transcription initiation factor IIB.
1991,
Pubmed
Helmann,
Structure and function of bacterial sigma factors.
1988,
Pubmed
Hisatake,
Conserved structural motifs between Xenopus and human TFIIB.
1991,
Pubmed
,
Xenbase
Hoey,
Isolation and characterization of the Drosophila gene encoding the TATA box binding protein, TFIID.
1990,
Pubmed
Hoffman,
Highly conserved core domain and unique N terminus with presumptive regulatory motifs in a human TATA factor (TFIID).
1990,
Pubmed
Horikoshi,
Mechanism of action of a yeast activator: direct effect of GAL4 derivatives on mammalian TFIID-promoter interactions.
1988,
Pubmed
Horikoshi,
Nucleotide and amino acid sequence of RAP30.
1991,
Pubmed
Horikoshi,
Transcription factor ATF interacts with the TATA factor to facilitate establishment of a preinitiation complex.
1988,
Pubmed
Horikoshi,
Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box.
1989,
Pubmed
Horikoshi,
Analysis of structure-function relationships of yeast TATA box binding factor TFIID.
1990,
Pubmed
Inostroza,
Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of general transcription factor IIE.
1991,
Pubmed
Kao,
Cloning of a transcriptionally active human TATA binding factor.
1990,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1987,
Pubmed
Lin,
Mechanism of action of an acidic transcriptional activator in vitro.
1991,
Pubmed
Maldonado,
Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex.
1990,
Pubmed
Malik,
Sequence of general transcription factor TFIIB and relationships to other initiation factors.
1991,
Pubmed
McCracken,
Related RNA polymerase-binding regions in human RAP30/74 and Escherichia coli sigma 70.
1991,
Pubmed
Muhich,
cDNA clone encoding Drosophila transcription factor TFIID.
1990,
Pubmed
Ohkuma,
Structural motifs and potential sigma homologies in the large subunit of human general transcription factor TFIIE.
1991,
Pubmed
Parker,
A Drosophila RNA polymerase II transcription factor contains a promoter-region-specific DNA-binding activity.
1984,
Pubmed
Peterson,
Functional domains and upstream activation properties of cloned human TATA binding protein.
1990,
Pubmed
Peterson,
Structure and functional properties of human general transcription factor IIE.
1991,
Pubmed
Poole,
The engrailed locus of Drosophila: structural analysis of an embryonic transcript.
1985,
Pubmed
Ptashne,
Activators and targets.
1990,
Pubmed
Reddy,
Dominant negative mutations in yeast TFIID define a bipartite DNA-binding region.
1991,
Pubmed
Reinberg,
Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of initiation factors IIB and IIE.
1987,
Pubmed
Roeder,
The complexities of eukaryotic transcription initiation: regulation of preinitiation complex assembly.
1991,
Pubmed
Sawadogo,
Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.
1985,
Pubmed
Scott,
The segmentation and homeotic gene network in early Drosophila development.
1987,
Pubmed
Sharp,
Gene transcription. TFIIB or not TFIIB?
1991,
Pubmed
Sopta,
Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II.
1989,
Pubmed
Storz,
Transcriptional regulator of oxidative stress-inducible genes: direct activation by oxidation.
1990,
Pubmed
Stringer,
Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID.
1990,
Pubmed
Struhl,
Molecular mechanisms of transcriptional regulation in yeast.
1989,
Pubmed
Studier,
Use of T7 RNA polymerase to direct expression of cloned genes.
1990,
Pubmed
Sumimoto,
Conserved sequence motifs in the small subunit of human general transcription factor TFIIE.
1991,
Pubmed
Sumimoto,
Factors involved in specific transcription by mammalian RNA polymerase II: identification of general transcription factor TFIIG.
1990,
Pubmed
Van Dyke,
Physical analysis of transcription preinitiation complex assembly on a class II gene promoter.
1988,
Pubmed
Wampler,
Fractionation of the general RNA polymerase II transcription factors from Drosophila embryos.
1990,
Pubmed
Workman,
Transcriptional regulation by the immediate early protein of pseudorabies virus during in vitro nucleosome assembly.
1988,
Pubmed
,
Xenbase
Yamamoto,
A bipartite DNA binding domain composed of direct repeats in the TATA box binding factor TFIID.
1992,
Pubmed
