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Proc Natl Acad Sci U S A
2005 Feb 08;1026:1927-32. doi: 10.1073/pnas.0409472102.
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Identification of the Wnt signaling activator leucine-rich repeat in Flightless interaction protein 2 by a genome-wide functional analysis.
Liu J
,
Bang AG
,
Kintner C
,
Orth AP
,
Chanda SK
,
Ding S
,
Schultz PG
.
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The Wnt signaling pathway acts ubiquitously in metazoans to control various aspects of embryonic development. Wnt ligands bind their receptors Frizzled and low-density lipoprotein receptor-related protein 5/6 and function through Disheveled (Dvl), Axin, adenomatous polyposis coli, glycogen synthase kinase 3beta, and casein kinase (CK) 1 to stabilize beta-catenin and induce lymphocyte enhancer-binding factor (LEF)/T cell factor (TCF)-dependent transcriptional activities. To identify previously unrecognized Wnt signaling modulators, a genome-wide functional screen was performed using large-scale arrayed cDNA collections. From this screen, both known components and previously uncharacterized regulators of this pathway were identified, including beta-catenin, Dvl1, Dvl3, Fbxw-1, Cul1, CK1epsilon, CK1delta, and gamma-catenin. In particular, a previously unrecognized activator, LRRFIP2 (leucine-rich repeat in Flightless interaction protein 2), was found that interacts with Dvl to increase the cellular levels of beta-catenin and activate beta-catenin/LEF/TCF-dependent transcriptional activity. The function of LRRFIP2 is blocked when a dominant negative Dvl (Xdd1) is coexpressed. Expression of LRRFIP2 in Xenopus embryos induced double axis formation and Wnt target gene expression; a dominant negative form of LRRFIP2 suppresses ectopic Wnt signaling in Xenopus embryos and partially inhibits endogenous dorsal axis formation. These data suggest that LRRFIP2 plays an important role in transducing Wnt signals.
Bienz,
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Bienz,
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Cardozo,
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,
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Conkright,
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Fong,
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Gao,
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,
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Glinka,
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,
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Gloy,
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,
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Häkli,
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He,
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Huang,
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Iourgenko,
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Kao,
The entire mesodermal mantle behaves as Spemann's organizer in dorsoanterior enhanced Xenopus laevis embryos.
1988,
Pubmed
,
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Korinek,
Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC-/- colon carcinoma.
1997,
Pubmed
Liu,
Siah-1 mediates a novel beta-catenin degradation pathway linking p53 to the adenomatous polyposis coli protein.
2001,
Pubmed
,
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Logan,
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Lu,
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2004,
Pubmed
Lum,
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2003,
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Moilanen,
Identification of a novel RING finger protein as a coregulator in steroid receptor-mediated gene transcription.
1998,
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Moon,
WNT and beta-catenin signalling: diseases and therapies.
2004,
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Park,
The planar cell-polarity gene stbm regulates cell behaviour and cell fate in vertebrate embryos.
2002,
Pubmed
,
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Peifer,
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2000,
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Peters,
Casein kinase I transduces Wnt signals.
1999,
Pubmed
,
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Pinson,
An LDL-receptor-related protein mediates Wnt signalling in mice.
2000,
Pubmed
Poukka,
The RING finger protein SNURF modulates nuclear trafficking of the androgen receptor.
2000,
Pubmed
Rousset,
Naked cuticle targets dishevelled to antagonize Wnt signal transduction.
2001,
Pubmed
Silacci,
Gelsolin superfamily proteins: key regulators of cellular functions.
2004,
Pubmed
Sokol,
Wnt signaling and dorso-ventral axis specification in vertebrates.
1999,
Pubmed
Sokol,
Analysis of Dishevelled signalling pathways during Xenopus development.
1996,
Pubmed
,
Xenbase
Sun,
PAR-1 is a Dishevelled-associated kinase and a positive regulator of Wnt signalling.
2001,
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,
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Tamai,
LDL-receptor-related proteins in Wnt signal transduction.
2000,
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,
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Warming,
Evi3, a common retroviral integration site in murine B-cell lymphoma, encodes an EBFAZ-related Krüppel-like zinc finger protein.
2003,
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,
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Wehrli,
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Wong,
Direct binding of the PDZ domain of Dishevelled to a conserved internal sequence in the C-terminal region of Frizzled.
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,
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Yan,
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,
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Zheng,
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