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Proc Natl Acad Sci U S A
1991 Oct 15;8820:9277-81.
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Germ cell-specific expression of a gene encoding eukaryotic translation elongation factor 1 alpha (eEF-1 alpha) and generation of eEF-1 alpha retropseudogenes in Xenopus laevis.
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We have studied by in situ hybridization the expression of the genes encoding the somatic form and the oocyte form of Xenopus laevis eEF-1 alpha. The somatic form of eEF-1 alpha (eEF-1 alpha S) mRNA is virtually undetectable in male and female germ cells of the adult gonad but is very abundant in embryonic cells after the neurula stage. In contrast, another form of eEF-1 alpha (eEF-1 alpha O) mRNA is highly concentrated in oogonia and in previtellogenic oocytes but is undetectable in eggs and embryos. eEF-1 alpha O mRNA is also present in spermatogonia and spermatocytes of adult testis. The latter finding identifies eEF-1 alpha O mRNA as a germ cell-specific gene product. Although germ cells contain very little eEF-1 alpha S mRNA, several eEF-1 alpha S retropseudogenes exist in X. laevis chromosomes. These genes are thought to arise in germ cells from reverse transcription of mRNA and subsequent integration of the cDNA copies into chromosomal DNA. We suggest that eEF-1 alpha S pseudogenes are generated in primordial germ cells of the embryo before they differentiate into oogonia or spermatogonia.
Davies,
A strategy to detect and isolate an intron-containing gene in the presence of multiple processed pseudogenes.
1989, Pubmed
Davies,
A strategy to detect and isolate an intron-containing gene in the presence of multiple processed pseudogenes.
1989,
Pubmed
Djé,
Three genes under different developmental control encode elongation factor 1-alpha in Xenopus laevis.
1990,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Ford,
Different sequences for 5S RNA in kidney cells and ovaries of Xenopus laevis.
1973,
Pubmed
,
Xenbase
Freytag,
Molecular structures of human argininosuccinate synthetase pseudogenes. Evolutionary and mechanistic implications.
1984,
Pubmed
Hay,
A protein component of Drosophila polar granules is encoded by vasa and has extensive sequence similarity to ATP-dependent helicases.
1988,
Pubmed
Hollis,
Processed genes: a dispersed human immunoglobulin gene bearing evidence of RNA-type processing.
1982,
Pubmed
Hourdry,
Localization of c-myc expression during oogenesis and embryonic development in Xenopus laevis.
1988,
Pubmed
,
Xenbase
Kalt,
Observations on early germ cell development and premeiotic ribosomal DNA amplification in Xenopus laevis.
1974,
Pubmed
,
Xenbase
Krieg,
The mRNA encoding elongation factor 1-alpha (EF-1 alpha) is a major transcript at the midblastula transition in Xenopus.
1989,
Pubmed
,
Xenbase
Krieg,
Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs.
1985,
Pubmed
,
Xenbase
Lasko,
The product of the Drosophila gene vasa is very similar to eukaryotic initiation factor-4A.
1988,
Pubmed
Lasko,
Posterior localization of vasa protein correlates with, but is not sufficient for, pole cell development.
1990,
Pubmed
Lemischka,
The sequences of an expressed rat alpha-tubulin gene and a pseudogene with an inserted repetitive element.
1982,
Pubmed
Madsen,
Retropseudogenes constitute the major part of the human elongation factor 1 alpha gene family.
1990,
Pubmed
Moldave,
Eukaryotic protein synthesis.
1985,
Pubmed
Nishioka,
Unusual alpha-globin-like gene that has cleanly lost both globin intervening sequences.
1980,
Pubmed
Pöting,
Two different mRNAs coding for identical elongation factor 1 alpha (EF-1 alpha) polypeptides in Xenopus laevis embryos.
1990,
Pubmed
,
Xenbase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Stutz,
Oocyte and somatic tyrosine tRNA genes in Xenopus laevis.
1989,
Pubmed
,
Xenbase
Tso,
Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene.
1985,
Pubmed
Uetsuki,
Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor-1 alpha.
1989,
Pubmed
Vanin,
Processed pseudogenes: characteristics and evolution.
1985,
Pubmed
Vanin,
A mouse alpha-globin-related pseudogene lacking intervening sequences.
1980,
Pubmed
Viel,
Elongation factor 1 alpha (EF-1 alpha) is concentrated in the Balbiani body and accumulates coordinately with the ribosomes during oogenesis of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Wegnez,
Sequence heterogeneity of 5 S RNA in Xenopus laevis.
1972,
Pubmed
,
Xenbase
Weiner,
Nonviral retroposons: genes, pseudogenes, and transposable elements generated by the reverse flow of genetic information.
1986,
Pubmed
Wormington,
Onset of 5 S RNA gene regulation during Xenopus embryogenesis.
1983,
Pubmed
,
Xenbase
