Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Localization of dystrophin relative to acetylcholine receptor domains in electric tissue and adult and cultured skeletal muscle.
Sealock R
,
Butler MH
,
Kramarcy NR
,
Gao KX
,
Murnane AA
,
Douville K
,
Froehner SC
.
???displayArticle.abstract???
Two high-affinity mAbs were prepared against Torpedo dystrophin, an electric organ protein that is closely similar to human dystrophin, the gene product of the Duchenne muscular dystrophy locus. The antibodies were used to localize dystrophin relative to acetylcholine receptors (AChR) in electric organ and in skeletal muscle, and to show identity between Torpedo dystrophin and the previously described 270/300-kD Torpedo postsynaptic protein. Dystrophin was found in both AChR-rich and AChR-poor regions of the innervated face of the electroplaque. Immunogold experiments showed that AChR and dystrophin were closely intermingled in the AChR domains. In contrast, dystrophin appeared to be absent from many or all AChR-rich domains of the rat neuromuscular junction and of AChR clusters in cultured muscle (Xenopus laevis). It was present, however, in the immediately surrounding membrane (deep regions of the junctional folds, membrane domains interdigitating with and surrounding AChR domains within clusters). These results suggest that dystrophin may have a role in organization of AChR in electric tissue. Dystrophin is not, however, an obligatory component of AChR domains in muscle and, at the neuromuscular junction, its roles may be more related to organization of the junctional folds.
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977, Pubmed,
Xenbase
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Anderson,
Aggregates of acetylcholine receptors are associated with plaques of a basal lamina heparan sulfate proteoglycan on the surface of skeletal muscle fibers.
1983,
Pubmed
,
Xenbase
Anderson,
Nerve-induced remodeling of muscle basal lamina during synaptogenesis.
1986,
Pubmed
,
Xenbase
Appleyard,
Monoclonal antibodies detect a spectrin-like protein in normal and dystrophic human skeletal muscle.
1984,
Pubmed
Bloch,
Molecular events in synaptogenesis: nerve-muscle adhesion and postsynaptic differentiation.
1988,
Pubmed
Bloch,
An unusual beta-spectrin associated with clustered acetylcholine receptors.
1989,
Pubmed
Bloch,
Cytoskeletal components of the vertebrate neuromuscular junction: vinculin, alpha-actinin, and filamin.
1983,
Pubmed
,
Xenbase
Bloch,
Isolation of acetylcholine receptor clusters in substrate-associated material from cultured rat myotubes using saponin.
1984,
Pubmed
Bridgman,
Visualization of the cytoplasmic surface of Torpedo postsynaptic membranes by freeze-etch and immunoelectron microscopy.
1987,
Pubmed
Buckle,
Localisation of a dystrophin-related autosomal gene to 6q24 in man, and to mouse chromosome 10 in the region of the dystrophia muscularis (dy) locus.
1990,
Pubmed
Byers,
Sequence similarity of the amino-terminal domain of Drosophila beta spectrin to alpha actinin and dystrophin.
1989,
Pubmed
Carr,
A novel 87,000-Mr protein associated with acetylcholine receptors in Torpedo electric organ and vertebrate skeletal muscle.
1989,
Pubmed
Chang,
Dystrophin in electric organ of Torpedo californica homologous to that in human muscle.
1989,
Pubmed
Chelly,
Transcription of the dystrophin gene in human muscle and non-muscle tissue.
1988,
Pubmed
Chen,
A protein homologous to the Torpedo postsynaptic 58K protein is present at the myotendinous junction.
1990,
Pubmed
,
Xenbase
Cullen,
Ultrastructural localization of dystrophin in human muscle by using gold immunolabelling.
1990,
Pubmed
Ervasti,
Deficiency of a glycoprotein component of the dystrophin complex in dystrophic muscle.
1990,
Pubmed
Fardeau,
[Presence of dystrophine-like protein at the neuromuscular junction in Duchenne muscular dystrophy and in "mdx" mutant mice].
1990,
Pubmed
Fertuck,
Quantitation of junctional and extrajunctional acetylcholine receptors by electron microscope autoradiography after 125I-alpha-bungarotoxin binding at mouse neuromuscular junctions.
1976,
Pubmed
Fong,
Increased activity of calcium leak channels in myotubes of Duchenne human and mdx mouse origin.
1990,
Pubmed
Fox,
The developmental morphology of Torpedo marmorata: electric organ--myogenic phase.
1978,
Pubmed
Franco,
Calcium entry through stretch-inactivated ion channels in mdx myotubes.
1990,
Pubmed
Froehner,
The postsynaptic 43K protein clusters muscle nicotinic acetylcholine receptors in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Froehner,
Peripheral proteins of postsynaptic membranes from Torpedo electric organ identified with monoclonal antibodies.
1984,
Pubmed
Froehner,
A postsynaptic Mr 58,000 (58K) protein concentrated at acetylcholine receptor-rich sites in Torpedo electroplaques and skeletal muscle.
1987,
Pubmed
,
Xenbase
Froehner,
Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor.
1983,
Pubmed
Giloh,
Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate.
1982,
Pubmed
Hammonds,
Protein sequence of DMD gene is related to actin-binding domain of alpha-actinin.
1987,
Pubmed
Heuser,
Organization of acetylcholine receptors in quick-frozen, deep-etched, and rotary-replicated Torpedo postsynaptic membrane.
1979,
Pubmed
Hirokawa,
Internal and external differentiations of the postsynaptic membrane at the neuromuscular junction.
1982,
Pubmed
Hoffman,
Detection of a specific isoform of alpha-actinin with antisera directed against dystrophin.
1989,
Pubmed
Hoffman,
Dystrophin: the protein product of the Duchenne muscular dystrophy locus.
1987,
Pubmed
Hoffman,
Dystrophin abnormalities in Duchenne/Becker muscular dystrophy.
1989,
Pubmed
Jasmin,
Asymmetric distribution of dystrophin in developing and adult Torpedo marmorata electrocyte: evidence for its association with the acetylcholine receptor-rich membrane.
1990,
Pubmed
Khurana,
Identification of a chromosome 6-encoded dystrophin-related protein.
1990,
Pubmed
Kingsley,
Preparation of cultured mammalian cells for transmission and scanning electron microscopy using Aclar film.
1988,
Pubmed
Koenig,
The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein.
1988,
Pubmed
Kordeli,
Evidence for a polarity in the distribution of proteins from the cytoskeleton in Torpedo marmorata electrocytes.
1986,
Pubmed
Kramarcy,
Dystrophin as a focal adhesion protein. Collocalization with talin and the Mr 48,000 sarcolemmal protein in cultured Xenopus muscle.
1990,
Pubmed
,
Xenbase
LaRochelle,
Determination of the tissue distributions and relative concentrations of the postsynaptic 43-kDa protein and the acetylcholine receptor in Torpedo.
1986,
Pubmed
Mandel,
Dystrophin. The gene and its product.
1989,
Pubmed
MAYOR,
A simple method for removing the resin from epoxy-embedded tissue.
1961,
Pubmed
Miranda,
Immunocytochemical study of dystrophin in muscle cultures from patients with Duchenne muscular dystrophy and unaffected control patients.
1988,
Pubmed
Mitra,
Three-dimensional structure of the nicotinic acetylcholine receptor and location of the major associated 43-kD cytoskeletal protein, determined at 22 A by low dose electron microscopy and x-ray diffraction to 12.5 A.
1989,
Pubmed
Mongini,
Free cytoplasmic Ca++ at rest and after cholinergic stimulus is increased in cultured muscle cells from Duchenne muscular dystrophy patients.
1988,
Pubmed
Morton,
The alpha 1 and alpha 2 polypeptides of the dihydropyridine-sensitive calcium channel differ in developmental expression and tissue distribution.
1989,
Pubmed
Peng,
Association of the postsynaptic 43K protein with newly formed acetylcholine receptor clusters in cultured muscle cells.
1985,
Pubmed
,
Xenbase
Peng,
Membrane particle aggregates in innervated and noninnervated cultures of Xenopus embryonic muscle cells.
1978,
Pubmed
,
Xenbase
Porter,
Characterization and localization of the Mr = 43,000 proteins associated with acetylcholine receptor-rich membranes.
1983,
Pubmed
Pumplin,
Acetylcholine receptor clusters of rat myotubes have at least three domains with distinctive cytoskeletal and membranous components.
1989,
Pubmed
Ravdin,
Fluorescent tetramethyl rhodamine derivatives of alpha-bungarotoxin: preparation, separation, and characterization.
1977,
Pubmed
Rowland,
Biochemistry of muscle membranes in Duchenne muscular dystrophy.
1980,
Pubmed
Sahr,
The complete cDNA and polypeptide sequences of human erythroid alpha-spectrin.
1990,
Pubmed
Sakakibara,
Duchenne dystrophy: ultrastructural localization of the acetylcholine receptor and intracellular microelectrode studies of neuromuscular transmission.
1977,
Pubmed
Sealock,
Postsynaptic distribution of acetylcholine receptors in electroplax of the torpedine ray, Narcine brasiliensis.
1980,
Pubmed
Sealock,
Ultrastructural localization of the Mr 43,000 protein and the acetylcholine receptor in Torpedo postsynaptic membranes using monoclonal antibodies.
1984,
Pubmed
Sealock,
Identification of regions of high acetylcholine receptor density in tannic acid-fixed postsynaptic membranes from electric tissue.
1980,
Pubmed
Sicinski,
The molecular basis of muscular dystrophy in the mdx mouse: a point mutation.
1989,
Pubmed
Sottrup-Jensen,
Primary structure of human alpha 2-macroglobulin. V. The complete structure.
1984,
Pubmed
Torres,
The mutant mdx: inherited myopathy in the mouse. Morphological studies of nerves, muscles and end-plates.
1987,
Pubmed
Watkins,
Immunoelectron microscopic localization of dystrophin in myofibres.
1988,
Pubmed
Winkelmann,
Full-length sequence of the cDNA for human erythroid beta-spectrin.
1990,
Pubmed
Witkowski,
Dystrophin-related muscular dystrophies.
1989,
Pubmed
Woodruff,
300-kD subsynaptic protein copurifies with acetylcholine receptor-rich membranes and is concentrated at neuromuscular synapses.
1987,
Pubmed
Wray,
Ultrastructural immunocytochemistry of particulate fractions using polyvinyl chloride microculture wells.
1984,
Pubmed
Yamaguchi,
Detection of a fast isoform of C-protein with an antiserum directed against the N-terminal portion of dystrophin.
1990,
Pubmed
