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Enhancers for RNA polymerase I in mouse ribosomal DNA.
Pikaard CS
,
Pape LK
,
Henderson SL
,
Ryan K
,
Paalman MH
,
Lopata MA
,
Reeder RH
,
Sollner-Webb B
.
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The intergenic spacer of the mouse ribosomal genes contains repetitive 140-base-pair (bp) elements which we show are enhancers for RNA polymerase I transcription analogous to the 60/81-bp repetitive enhancers (enhancers containing a 60-bp and an 81-bp element) previously characterized from Xenopus laevis. In rodent cell transfection assays, the 140-bp repeats stimulated an adjacent mouse polymerase I promoter when located in cis and competed with it when located in trans. Remarkably, in frog oocyte injection assays, the 140-bp repeats enhanced a frog ribosomal gene promoter as strongly as did the homologous 60/81-bp repeats. Mouse 140-bp repeats also competed against frog promoters in trans. The 140-bp repeats bound UBF, a DNA-binding protein we have purified from mouse extracts that is the mouse homolog of polymerase I transcription factors previously isolated from frogs and humans. The DNA-binding properties of UBF are conserved from the mouse to the frog. The same regulatory elements (terminators, gene and spacer promoters, and enhancers) have now been identified in both a mammalian and an amphibian spacer, and they are found in the same relative order. Therefore, this arrangement of elements probably is widespread in nature and has important functional consequences.
Barker,
Structure and evolution of the intergenic region in a ribosomal DNA repeat unit of wheat.
1988, Pubmed
Barker,
Structure and evolution of the intergenic region in a ribosomal DNA repeat unit of wheat.
1988,
Pubmed
Bell,
Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.
1990,
Pubmed
Bell,
Functional cooperativity between transcription factors UBF1 and SL1 mediates human ribosomal RNA synthesis.
1988,
Pubmed
Bell,
Molecular mechanisms governing species-specific transcription of ribosomal RNA.
1989,
Pubmed
,
Xenbase
Boseley,
Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Busby,
Spacer sequences regulate transcription of ribosomal gene plasmids injected into Xenopus embryos.
1983,
Pubmed
,
Xenbase
Cassidy,
Transcriptional role for the nontranscribed spacer of rat ribosomal DNA.
1986,
Pubmed
Cassidy,
Additional RNA polymerase I initiation site within the nontranscribed spacer region of the rat rRNA gene.
1987,
Pubmed
Chikaraishi,
Genomic organization of rat rDNA.
1983,
Pubmed
Culotta,
Mouse and frog violate the paradigm of species-specific transcription of ribosomal RNA genes.
1987,
Pubmed
,
Xenbase
De Winter,
Spacer promoters are essential for efficient enhancement of X. laevis ribosomal transcription.
1986,
Pubmed
,
Xenbase
De Winter,
A complex array of sequences enhances ribosomal transcription in Xenopus laevis.
1987,
Pubmed
,
Xenbase
Dixit,
A cis-acting sequence within the rat ribosomal DNA enhancer region can modulate RNA polymerase II-directed transcription of the metallothionein I gene in vitro.
1989,
Pubmed
Dixit,
An enhancer element in the far upstream spacer region of rat ribosomal RNA gene.
1987,
Pubmed
Dumenco,
Characterization of the region around the start point of transcription of ribosomal RNA in the Chinese hamster.
1986,
Pubmed
Dunaway,
Transactivation of the Xenopus rRNA gene promoter by its enhancer.
1989,
Pubmed
,
Xenbase
Dunaway,
A transcription factor, TFIS, interacts with both the promoter and enhancer of the Xenopus rRNA genes.
1989,
Pubmed
,
Xenbase
Elion,
An RNA polymerase I enhancer in Saccharomyces cerevisiae.
1986,
Pubmed
Evans,
An erythrocyte-specific DNA-binding factor recognizes a regulatory sequence common to all chicken globin genes.
1988,
Pubmed
Galas,
DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
1978,
Pubmed
Garg,
A 37-base pair element in the far upstream spacer region can enhance transcription of rat rDNA in vitro and can bind to the core promoter-binding factor(s).
1989,
Pubmed
Grimaldi,
Multiple repeated units in Drosophila melanogaster ribosomal DNA spacer stimulate rRNA precursor transcription.
1988,
Pubmed
Grummt,
Ribosomal RNA transcription in vitro is species specific.
1982,
Pubmed
Grummt,
Transcription of mouse rDNA terminates downstream of the 3' end of 28S RNA and involves interaction of factors with repeated sequences in the 3' spacer.
1985,
Pubmed
Grummt,
A transcription terminator located upstream of the mouse rDNA initiation site affects rRNA synthesis.
1986,
Pubmed
Henderson,
A transcriptional terminator is a novel element of the promoter of the mouse ribosomal RNA gene.
1986,
Pubmed
Henderson,
The promoter-proximal rDNA terminator augments initiation by preventing disruption of the stable transcription complex caused by polymerase read-in.
1989,
Pubmed
Jantzen,
Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins.
1990,
Pubmed
Kuehn,
Nucleotide sequence of the genetically labile repeated elements 5' to the origin of mouse rRNA transcription.
1983,
Pubmed
,
Xenbase
Kuhn,
A novel promoter in the mouse rDNA spacer is active in vivo and in vitro.
1987,
Pubmed
Labhart,
Enhancer-like properties of the 60/81 bp elements in the ribosomal gene spacer of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Labhart,
Characterization of three sites of RNA 3' end formation in the Xenopus ribosomal gene spacer.
1986,
Pubmed
,
Xenbase
Labhart,
A point mutation uncouples RNA 3'-end formation and termination during ribosomal gene transcription in Xenopus laevis.
1990,
Pubmed
,
Xenbase
Landschulz,
Isolation of a recombinant copy of the gene encoding C/EBP.
1988,
Pubmed
Learned,
Human rRNA transcription is modulated by the coordinate binding of two factors to an upstream control element.
1986,
Pubmed
McStay,
A termination site for Xenopus RNA polymerase I also acts as an element of an adjacent promoter.
1986,
Pubmed
,
Xenbase
Miller,
A complex control region of the mouse rRNA gene directs accurate initiation by RNA polymerase I.
1985,
Pubmed
Mishima,
Fractionation and reconstitution of factors required for accurate transcription of mammalian ribosomal RNA genes: identification of a species-dependent initiation factor.
1982,
Pubmed
Morrow,
Proteins that bind to the yeast rDNA enhancer.
1989,
Pubmed
Moss,
The putative promoter of a Xenopus laevis ribosomal gene is reduplicated.
1979,
Pubmed
,
Xenbase
Moss,
The promotion of ribosomal transcription in eukaryotes.
1985,
Pubmed
Moss,
A transcriptional function for the repetitive ribosomal spacer in Xenopus laevis.
,
Pubmed
,
Xenbase
Murtif,
In vivo transcription of rDNA spacers in Drosophila.
1985,
Pubmed
Pape,
Half helical turn spacing changes convert a frog into a mouse rDNA promoter: a distant upstream domain determines the helix face of the initiation site.
1990,
Pubmed
,
Xenbase
Pape,
The Xenopus ribosomal DNA 60- and 81-base-pair repeats are position-dependent enhancers that function at the establishment of the preinitiation complex: analysis in vivo and in an enhancer-responsive in vitro system.
1989,
Pubmed
,
Xenbase
Peterson,
Purified mu EBP-E binds to immunoglobulin enhancers and promoters.
1988,
Pubmed
Pikaard,
rUBF, an RNA polymerase I transcription factor from rats, produces DNase I footprints identical to those produced by xUBF, its homolog from frogs.
1990,
Pubmed
,
Xenbase
Pikaard,
The Xenopus ribosomal gene enhancers bind an essential polymerase I transcription factor, xUBF.
1989,
Pubmed
,
Xenbase
Pikaard,
Sequence elements essential for function of the Xenopus laevis ribosomal DNA enhancers.
1988,
Pubmed
,
Xenbase
Reeder,
Regulatory elements of the generic ribosomal gene.
1989,
Pubmed
Reeder,
Mechanisms of nucleolar dominance in animals and plants.
1985,
Pubmed
Reeder,
Spacer regulation of Xenopus ribosomal gene transcription: competition in oocytes.
1983,
Pubmed
,
Xenbase
Reeder,
The mechanism of nucleolar dominance in Xenopus hybrids.
1984,
Pubmed
,
Xenbase
Sollner-Webb,
Accurate transcription of cloned Xenopus rRNA genes by RNA polymerase I: demonstration by S1 nuclease mapping.
1982,
Pubmed
,
Xenbase
Sollner-Webb,
Transcription of cloned eukaryotic ribosomal RNA genes.
1986,
Pubmed
Sollner-Webb,
The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
1979,
Pubmed
,
Xenbase
Tower,
Factors and nucleotide sequences that direct ribosomal DNA transcription and their relationship to the stable transcription complex.
1986,
Pubmed
Tower,
An RNA polymerase I promoter located in the CHO and mouse ribosomal DNA spacers: functional analysis and factor and sequence requirements.
1989,
Pubmed
Wilkinson,
Transcription of Xenopus ribosomal RNA genes by RNA polymerase I in vitro.
1982,
Pubmed
,
Xenbase
Yang-Yen,
Purification and characterization of a high-mobility-group-like DNA-binding protein that stimulates rRNA synthesis in vitro.
1988,
Pubmed
