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We have discovered a ring-shaped particle of 12.5 nm diameter, 14.5S and apparent molecular weight of approximately 570,000 that displays 6-fold radial symmetry and is composed of a single kind of an acidic (pI approximately 5.5) polypeptide of Mr 97,000 (p97). Using antibodies to this protein we have detected its occurrence in a wide range of cells and tissues of diverse species from frog to man, including highly specialized cells such as mammalian erythrocytes and spermatozoa. In Xenopus laevis oocytes, the particle is found in both isolated nuclei and in manually enucleated ooplasms, which corresponds to immunofluorescence staining dispersed over both nucleoplasm and cytoplasm. The particle has a N-ethylmaleimide (NEM)-inhibitable Mg2(+)-ATPase activity, and its amino acid sequence, as deduced from cDNA clones, displays considerable homology to the mammalian NEM-sensitive fusion protein (NSF) and yeast Sec18p believed to be essential for vesicle fusion in secretory processes, indicating that these three proteins belong to the same multigene family.
Aebersold,
Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.
1987, Pubmed
Aebersold,
Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.
1987,
Pubmed
Al-Awqati,
Proton-translocating ATPases.
1986,
Pubmed
Arrigo,
Identity of the 19S 'prosome' particle with the large multifunctional protease complex of mammalian cells (the proteasome).
1988,
Pubmed
Arrigo,
A 20S particle ubiquitous from yeast to human.
1987,
Pubmed
Arrigo,
Characterization of the prosome from Drosophila and its similarity to the cytoplasmic structures formed by the low molecular weight heat-shock proteins.
1985,
Pubmed
Bear,
Interactions of Escherichia coli transcription termination factor rho with RNA. II. Electron microscopy and nuclease protection experiments.
1988,
Pubmed
Block,
Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.
1988,
Pubmed
Bowman,
H+-ATPases from mitochondria, plasma membranes, and vacuoles of fungal cells.
1986,
Pubmed
Castaño,
Eukaryotic pre-tRNA 5' processing nuclease: copurification with a complex cylindrical particle.
1986,
Pubmed
,
Xenbase
Cohen,
The subunit structure of rabbit-skeletal-muscle phosphorylase kinase, and the molecular basis of its activation reactions.
1973,
Pubmed
Cohen,
The role of cyclic-AMP-dependent protein kinase in the regulation of glycogen metabolism in mammalian skeletal muscle.
1978,
Pubmed
Domae,
Donut-shaped "miniparticles" in nuclei of human and rat cells.
1982,
Pubmed
Eakle,
Characterization of a component of the yeast secretion machinery: identification of the SEC18 gene product.
1988,
Pubmed
Elder,
Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein.
1977,
Pubmed
Falkenburg,
Drosophila small cytoplasmic 19S ribonucleoprotein is homologous to the rat multicatalytic proteinase.
1988,
Pubmed
Franke,
Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates.
1979,
Pubmed
,
Xenbase
Fry,
ATP-binding site of adenylate kinase: mechanistic implications of its homology with ras-encoded p21, F1-ATPase, and other nucleotide-binding proteins.
1986,
Pubmed
Gill,
The generation of valosin-like peptides from a precursor protein in vitro as an extraction artifact.
1989,
Pubmed
GUENTHER,
[Electron-microscopic studies of the germinating spores of Funaria hygrometrica].
1960,
Pubmed
Harmon,
Purification and partial characterization of ring-shaped miniparticles.
1983,
Pubmed
Harris,
Biochemical and ultrastructural characterization of a high molecular weight soluble Mg2+ -ATPase from human erythrocytes.
1984,
Pubmed
Hemmingsen,
Homologous plant and bacterial proteins chaperone oligomeric protein assembly.
1988,
Pubmed
Hendrix,
Purification and properties of groE, a host protein involved in bacteriophage assembly.
1979,
Pubmed
Hohn,
Isolation and characterization of the host protein groE involved in bacteriophage lambda assembly.
1979,
Pubmed
Holmes,
Protein-RNA interactions during TMV assembly.
1979,
Pubmed
Hügle,
The 22 S cylinder particles of Xenopus laevis. II. Immunological characterization and localization of their proteins in tissues and cultured cells.
1983,
Pubmed
,
Xenbase
Hügle,
Ribocharin: a nuclear Mr 40,000 protein specific to precursor particles of the large ribosomal subunit.
1985,
Pubmed
,
Xenbase
Kapprell,
Identification of a basic protein of Mr 75,000 as an accessory desmosomal plaque protein in stratified and complex epithelia.
1988,
Pubmed
Kaufmann,
SDS-PAGE strongly overestimates the molecular masses of the neurofilament proteins.
1984,
Pubmed
Kleinschmidt,
Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Kleinschmidt,
The 22 S cylinder particles of Xenopus laevis. I. Biochemical and electron microscopic characterization.
1983,
Pubmed
,
Xenbase
Kleinschmidt,
Proteinase yscE of yeast shows homology with the 20 S cylinder particles of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Knapp,
Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation.
1989,
Pubmed
Koller,
Use of a cDNA clone to identify a supposed precursor protein containing valosin.
,
Pubmed
Konturek,
Valosin stimulates gastric and exocrine pancreatic secretion and inhibits fasting small intestinal myoelectric activity in the dog.
1987,
Pubmed
Kopun,
Nuclear glycogen synthase--fact or artifact?
1982,
Pubmed
Kremp,
Prosomes exist in plant cells too.
1986,
Pubmed
Krohne,
Proteins of pore complex--lamina structures from nuclei and nuclear membranes.
1983,
Pubmed
,
Xenbase
Krohne,
A major soluble acidic protein located in nuclei of diverse vertebrate species.
1980,
Pubmed
,
Xenbase
Krohne,
The conserved carboxy-terminal cysteine of nuclear lamins is essential for lamin association with the nuclear envelope.
1989,
Pubmed
,
Xenbase
Kuenzel,
Substrate specificity determinants for casein kinase II as deduced from studies with synthetic peptides.
1987,
Pubmed
Kyhse-Andersen,
Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose.
1984,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lewis,
Anomalous placement of introns in a member of the intermediate filament multigene family: an evolutionary conundrum.
1986,
Pubmed
Longo,
Basic proteins of the perinuclear theca of mammalian spermatozoa and spermatids: a novel class of cytoskeletal elements.
1987,
Pubmed
Malhotra,
Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stack.
1988,
Pubmed
Martins de Sa,
Prosomes. Ubiquity and inter-species structural variation.
1986,
Pubmed
McMullin,
A highly evolutionarily conserved mitochondrial protein is structurally related to the protein encoded by the Escherichia coli groEL gene.
1988,
Pubmed
,
Xenbase
Möller,
Phosphate-binding sequences in nucleotide-binding proteins.
1985,
Pubmed
Novick,
Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway.
1980,
Pubmed
Oda,
Observations on the structure of the termination factor rho and its attachment to DNA.
1972,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
1977,
Pubmed
Pearson,
Substrate specificity of a multifunctional calmodulin-dependent protein kinase.
1985,
Pubmed
Rebhun,
Electron microscope studies of glycogen synthase.
1973,
Pubmed
Riezman,
Endocytosis in yeast: several of the yeast secretory mutants are defective in endocytosis.
1985,
Pubmed
Roberts,
Termination factor for RNA synthesis.
1969,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schmid,
The prosome: an ubiquitous morphologically distinct RNP particle associated with repressed mRNPs and containing specific ScRNA and a characteristic set of proteins.
1984,
Pubmed
Schmidt,
Valosin: isolation and characterization of a novel peptide from porcine intestine.
1985,
Pubmed
Schuldt,
Analysis of cytoplasmic 19 S ring-type particles in Drosophila which contain hsp 23 at normal growth temperature.
1985,
Pubmed
Shur,
[The presence of glycogen synthase in phosphorylase kinase preparations isolated from rabbit skeletal muscles].
1986,
Pubmed
Stitt,
Absence of a phosphorylated intermediate during ATP hydrolysis by Escherichia coli transcription termination protein rho.
1986,
Pubmed
Tanaka,
Molecular organization of a high molecular weight multi-protease complex from rat liver.
1988,
Pubmed
Thomas,
An octamer of histones in chromatin and free in solution.
1975,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Tsukahara,
RNA degrading activity is tightly associated with the multicatalytic proteinase, ingensin.
1989,
Pubmed
Walker,
Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
1982,
Pubmed
Walsh,
Extended N-terminal sequencing of proteins of archaebacterial ribosomes blotted from two-dimensional gels onto glass fiber and poly(vinylidene difluoride) membrane.
1988,
Pubmed
Wessel,
A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids.
1984,
Pubmed
White,
Purification of a water-soluble Mg2+-ATPase from human erythrocyte membranes.
1980,
Pubmed
White,
A water-soluble Mg2+-ATPase from erythrocyte membranes.
1976,
Pubmed
Wilson,
A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast.
1989,
Pubmed
Woodgett,
Substrate specificity of protein kinase C. Use of synthetic peptides corresponding to physiological sites as probes for substrate recognition requirements.
1986,
Pubmed
