Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Xenopus transcription factor IIIA (TFIIIA) gene expression is stringently regulated during development. The steady-state level of TFIIIA mRNA in a somatic cell is approximately 10(6) times less than in an immature oocyte. We have undertaken studies designed to identify differences in how the TFIIIA gene is transcribed in oocytes and somatic cells. In this regard, we have localized an upstream transcriptional control element in the TFIIIA promoter that stimulates transcription from the TFIIIA promoter approximately threefold in microinjected oocytes. The upstream element, in cis. does not stimulate transcription from the TFIIIA promoter in somatic cells. Thus, the element appears to be oocyte specific in the context of the TFIIIA promoter. However, both oocytes and somatic cells contain a protein (or a related protein) that binds the upstream element. We have termed this protein from oocytes the TFIIIAdistal element factor. The sequence of the upstream element is similar to the sequence of the upstream element found in the adenovirus major late promoter that is a binding site for the major late transcription factor. By gel shift analysis, chemical footprinting, methylation intereference, and point mutation analysis, we demonstrate that the TFIIIAdistal element factor and major late transcription factor have similar DNA-binding properties.
Andrews,
Transient activation of oocyte 5S RNA genes in Xenopus embryos by raising the level of the trans-acting factor TFIIIA.
1987, Pubmed,
Xenbase
Andrews,
Transient activation of oocyte 5S RNA genes in Xenopus embryos by raising the level of the trans-acting factor TFIIIA.
1987,
Pubmed
,
Xenbase
Bram,
Isolation of a Saccharomyces cerevisiae centromere DNA-binding protein, its human homolog, and its possible role as a transcription factor.
1987,
Pubmed
Brown,
A positive transcription factor controls the differential expression of two 5S RNA genes.
1985,
Pubmed
,
Xenbase
Carthew,
An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.
1985,
Pubmed
Carthew,
The major late transcription factor binds to and activates the mouse metallothionein I promoter.
1987,
Pubmed
Chodosh,
The adenovirus major late transcription factor activates the rat gamma-fibrinogen promoter.
1987,
Pubmed
Chodosh,
A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.
1986,
Pubmed
Chodosh,
A yeast protein possesses the DNA-binding properties of the adenovirus major late transcription factor.
1989,
Pubmed
Chu,
Electroporation for the efficient transfection of mammalian cells with DNA.
1987,
Pubmed
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Ginsberg,
Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.
1984,
Pubmed
,
Xenbase
Gonzalez,
A cluster of phosphorylation sites on the cyclic AMP-regulated nuclear factor CREB predicted by its sequence.
1989,
Pubmed
Harland,
Stability of RNA in developing Xenopus embryos and identification of a destabilizing sequence in TFIIIA messenger RNA.
1988,
Pubmed
,
Xenbase
Hen,
Sequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter.
1982,
Pubmed
Hoeffler,
Activation of transcription factor IIIC by the adenovirus E1A protein.
1988,
Pubmed
Johnston,
Competitive inhibition of hsp70 gene expression causes thermosensitivity.
1988,
Pubmed
Kuwabara,
Footprinting DNA-protein complexes in situ following gel retardation assays using 1,10-phenanthroline-copper ion: Escherichia coli RNA polymerase-lac promoter complexes.
1987,
Pubmed
Lenardo,
NF-kappa B protein purification from bovine spleen: nucleotide stimulation and binding site specificity.
1988,
Pubmed
Matsumoto,
Upstream sequences required for transcription of the TFIIIA gene in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
McKnight,
Transcriptional control signals of a eukaryotic protein-coding gene.
1982,
Pubmed
Minth,
Characterization, sequence, and expression of the cloned human neuropeptide Y gene.
1986,
Pubmed
Miyamoto,
Specific interaction between a transcription factor and the upstream element of the adenovirus-2 major late promoter.
1985,
Pubmed
Morgan,
Sp1, a CAAT-binding factor, and the adenovirus major late promoter transcription factor interact with functional regions of the gamma-fibrinogen promoter.
1988,
Pubmed
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Peterson,
Complex protein binding within the mouse immunoglobulin heavy-chain enhancer.
1987,
Pubmed
Sakonju,
Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
1982,
Pubmed
,
Xenbase
Sawadogo,
Multiple forms of the human gene-specific transcription factor USF. I. Complete purification and identification of USF from HeLa cell nuclei.
1988,
Pubmed
Sawadogo,
Multiple forms of the human gene-specific transcription factor USF. II. DNA binding properties and transcriptional activity of the purified HeLa USF.
1988,
Pubmed
Scheidereit,
A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein.
1988,
Pubmed
Scholer,
In vivo competition between a metallothionein regulatory element and the SV40 enhancer.
1986,
Pubmed
Scotto,
Positive and negative regulation of the gene for transcription factor IIIA in Xenopus laevis oocytes.
1989,
Pubmed
,
Xenbase
Sealey,
At least two nuclear proteins bind specifically to the Rous sarcoma virus long terminal repeat enhancer.
1987,
Pubmed
Shapiro,
A high-efficiency HeLa cell nuclear transcription extract.
1988,
Pubmed
,
Xenbase
Shastry,
Altered levels of a 5 S gene-specific transcription factor (TFIIIA) during oogenesis and embryonic development of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Taylor,
The developmental expression of the gene for TFIIIA in Xenopus laevis.
1986,
Pubmed
,
Xenbase
Treisman,
cis and trans activation of globin gene transcription in transient assays.
1983,
Pubmed
Tso,
Structure of the gene for Xenopus transcription factor TFIIIA.
1986,
Pubmed
,
Xenbase
Weintraub,
Expression of transfected DNA depends on DNA topology.
1986,
Pubmed
Wolffe,
Developmental regulation of two 5S ribosomal RNA genes.
1988,
Pubmed
,
Xenbase
