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Mol Cell Biol
1989 Oct 01;910:4187-95. doi: 10.1128/mcb.9.10.4187-4195.1989.
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Functional expression of mammalian glucose transporters in Xenopus laevis oocytes: evidence for cell-dependent insulin sensitivity.
Vera JC
,
Rosen OM
.
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We report the functional expression of two different mammalian facilitative glucose transporters in Xenopus oocytes. The RNAs encoding the rat brain and liver glucose transporters were transcribed in vitro and microinjected into Xenopus oocytes. Microinjected cells showed a marked increase in 2-deoxy-D-glucose uptake as compared with controls injected with water. 2-Deoxy-D-glucose uptake increased during the 5 days after microinjection of the RNAs, and the microinjected RNAs were stable for at least 3 days. The expression of functional glucose transporters was dependent on the amount of RNA injected. The oocyte-expressed transporters could be immunoprecipitated with anti-brain and anti-liver glucose transporter-specific antibodies. Uninjected oocytes expressed an endogenous transporter that appeared to be stereospecific and inhibitable by cytochalasin B. This transporter was kinetically and immunologically distinguishable from both rat brain and liver glucose transporters. The uniqueness of this transporter was confirmed by Northern (RNA) blot analysis. The endogenous oocyte transporter was responsive to insulin and to insulinlike growth factor I. Most interestingly, both the rat brain and liver glucose transporters, which were not insulin sensitive in the tissues from which they were cloned, responded to insulin in the oocyte similarly to the endogenous oocyte transporter. These data suggest that the insulin responsiveness of a given glucose transporter depends on the type of cell in which the protein is expressed. The expression of hexose transporters in the microinjected oocytes may help to identify tissue-specific molecules involved in hormonal alterations in hexose transport activity.
Asano,
Rabbit brain glucose transporter responds to insulin when expressed in insulin-sensitive Chinese hamster ovary cells.
1989, Pubmed
Asano,
Rabbit brain glucose transporter responds to insulin when expressed in insulin-sensitive Chinese hamster ovary cells.
1989,
Pubmed
Axelrod,
Unique cytochalasin B binding characteristics of the hepatic glucose carrier.
1983,
Pubmed
Baly,
Dissociation of insulin-stimulated glucose transport from the translocation of glucose carriers in rat adipose cells.
1987,
Pubmed
Birnbaum,
Cloning and characterization of a cDNA encoding the rat brain glucose-transporter protein.
1986,
Pubmed
Blok,
Insulin-induced translocation of glucose transporters from post-Golgi compartments to the plasma membrane of 3T3-L1 adipocytes.
1988,
Pubmed
Carter-Su,
Reconstitution of D-glucose transport activity from cytoplasmic membranes. Evidence against recruitment of cytoplasmic membrane transporters into the plasma membrane as the sole action of insulin.
1980,
Pubmed
Carter-Su,
Photoaffinity labeling of the human erythrocyte D-glucose transporter.
1982,
Pubmed
Cushman,
Potential mechanism of insulin action on glucose transport in the isolated rat adipose cell. Apparent translocation of intracellular transport systems to the plasma membrane.
1980,
Pubmed
Czech,
Characterization of (3H)cytochalasin B binding to the fat cell plasma membrane.
1976,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
El-Etr,
Meiotic maturation in Xenopus laevis oocytes initiated by insulin.
1979,
Pubmed
,
Xenbase
El-Etr,
The Role of Zinc and Follicle Cells in Insulin-Initiated Meiotic Maturation of Xenopus laevis Oocytes.
1980,
Pubmed
,
Xenbase
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Fukumoto,
Sequence, tissue distribution, and chromosomal localization of mRNA encoding a human glucose transporter-like protein.
1988,
Pubmed
Gorga,
Equilibria and kinetics of ligand binding to the human erythrocyte glucose transporter. Evidence for an alternating conformation model for transport.
1981,
Pubmed
Gould,
Insulin-stimulated translocation of the HepG2/erythrocyte-type glucose transporter expressed in 3T3-L1 adipocytes.
1989,
Pubmed
Haspel,
Characterization of antisera to a synthetic carboxyl-terminal peptide of the glucose transporter protein.
1988,
Pubmed
Haspel,
Biosynthetic precursors and in vitro translation products of the glucose transporter of human hepatocarcinoma cells, human fibroblasts, and murine preadipocytes.
1985,
Pubmed
Helgerson,
Equilibrium ligand binding to the human erythrocyte sugar transporter. Evidence for two sugar-binding sites per carrier.
1987,
Pubmed
James,
Molecular cloning and characterization of an insulin-regulatable glucose transporter.
1989,
Pubmed
Janicot,
Activation of glucose uptake by insulin and insulin-like growth factor I in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Jung,
Cytochalasin B binding sites and glucose transport carrier in human erythrocyte ghosts.
1977,
Pubmed
Karnieli,
Insulin-stimulated translocation of glucose transport systems in the isolated rat adipose cell. Time course, reversal, insulin concentration dependency, and relationship to glucose transport activity.
1981,
Pubmed
Kasahara,
Reconstitution and purification of the D-glucose transporter from human erythrocytes.
1977,
Pubmed
Kayano,
Evidence for a family of human glucose transporter-like proteins. Sequence and gene localization of a protein expressed in fetal skeletal muscle and other tissues.
1988,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Madar,
3-O-methyl-D-glucose uptake in isolated rat hepatocytes. Effects of dexamethasone.
1983,
Pubmed
Maller,
A study of the induction of cell division in amphibian oocytes by insulin.
1981,
Pubmed
,
Xenbase
Mueckler,
Sequence and structure of a human glucose transporter.
1985,
Pubmed
Nielsen,
Preparation of capped RNA transcripts using T7 RNA polymerase.
1986,
Pubmed
Oka,
Photoaffinity labeling of insulin-sensitive hexose transporters in intact rat adipocytes. Direct evidence that latent transporters become exposed to the extracellular space in response to insulin.
1984,
Pubmed
Sadler,
In vivo regulation of cyclic AMP phosphodiesterase in Xenopus oocytes. Stimulation by insulin and insulin-like growth factor 1.
1987,
Pubmed
,
Xenbase
Sadler,
Progesterone inhibits adenylate cyclase in Xenopus oocytes. Action on the guanine nucleotide regulatory protein.
1981,
Pubmed
,
Xenbase
Sarkar,
Expression of the human erythrocyte glucose transporter in Escherichia coli.
1988,
Pubmed
Shanahan,
Characterization of cytochalasin B photoincorporation into human erythrocyte D-glucose transporter and F-actin.
1983,
Pubmed
Simpson,
Hormonal regulation of mammalian glucose transport.
1986,
Pubmed
Suzuki,
Evidence that insulin causes translocation of glucose transport activity to the plasma membrane from an intracellular storage site.
1980,
Pubmed
Suzuki,
Reassessment of the translocation hypothesis by kinetic studies on hexose transport in isolated rat adipocytes.
1988,
Pubmed
Thorens,
Cloning and functional expression in bacteria of a novel glucose transporter present in liver, intestine, kidney, and beta-pancreatic islet cells.
1988,
Pubmed
Toyoda,
Reassessment of insulin effects on the Vmax and Km values of hexose transport in isolated rat epididymal adipocytes.
1987,
Pubmed
Wall,
Isolation of plasma membrane complexes from Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Wallace,
The role of zinc and follicle cells in insulin-initiated meiotic maturation of Xenopus laevis oocytes.
1980,
Pubmed
,
Xenbase
Wheeler,
The glucose transporter of mammalian cells.
1985,
Pubmed
Williams,
Stereospecific transport of glucose in the perfused rat liver.
1968,
Pubmed
