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We have used in vitro explant cultures of Xenopus laevis skin to investigate the role that the thyroid hormone triiodothyronine (T3) plays in activating the 63-kilodalton (kDa) keratin genes. The activation of these genes in vivo requires two distinct steps, one independent of T3 and one dependent on T3. In this report we have shown that the same two steps are required to fully activate the 63-kDa keratin genes in skin explant cultures, and we have characterized the T3-mediated step in greater detail. Unlike the induction of transcription by T3 or steroid hormones in adult tissues, there was a long latent period of approximately 2 days between the addition of T3 to skin cultures and an increase in concentration of keratin mRNA. While the T3 induction of 63-kDa keratin gene transcription cannot occur until age 48, a short transient exposure of stage 40 skin cultures to T3 resulted in high-level expression of these genes 5 days later, when normal siblings had reached stage 48. This result indicates that T3 induces a stable change in epidermal cells which can be expressed much later, after extensive cell proliferation has occurred in the absence of T3. Once the 63-kDa keratin genes were induced, they were stably expressed, and by the end of metamorphosis T3 had no further effect on their expression. The results suggest that T3 induces constitutive expression of the 63-kDa keratin genes during metamorphosis.
Amasino,
Acceleration of nucleic acid hybridization rate by polyethylene glycol.
1986, Pubmed
Amasino,
Acceleration of nucleic acid hybridization rate by polyethylene glycol.
1986,
Pubmed
Atchison,
The role of the kappa enhancer and its binding factor NF-kappa B in the developmental regulation of kappa gene transcription.
1987,
Pubmed
Casanova,
5'-Flanking DNA of the rat growth hormone gene mediates regulated expression by thyroid hormone.
1985,
Pubmed
Cattini,
The human growth hormone gene is negatively regulated by triiodothyronine when transfected into rat pituitary tumor cells.
1986,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Davis,
Expression of a single transfected cDNA converts fibroblasts to myoblasts.
1987,
Pubmed
DeFesi,
L-Triiodothyronine (T3) stimulates growth of cultured GC cells by action early in the G1 period: evidence for mediation by the nuclear T3 receptor.
1985,
Pubmed
de-la-Concha,
Functional interactions of neurogenic genes of Drosophila melanogaster.
1988,
Pubmed
Dierich,
Cell-specificity of the chicken ovalbumin and conalbumin promoters.
1987,
Pubmed
Di Liegro,
Cellular mechanism of action of thyroid hormones.
1987,
Pubmed
Ellison,
Developmental changes in keratin patterns during epidermal maturation.
1985,
Pubmed
,
Xenbase
Fox,
Genesis and regression of the figures of Eberth and occurrence of cytokeratin aggregates in the epidermis of anuran larvae.
1986,
Pubmed
,
Xenbase
HEADY,
HORMONAL MODIFICATION OF THE DEVELOPMENT OF PLICAL SKIN GLANDS.
1964,
Pubmed
Hoffmann,
Amino acid sequence of the carboxy-terminal part of an acidic type I cytokeratin of molecular weight 51 000 from Xenopus laevis epidermis as predicted from the cDNA sequence.
1984,
Pubmed
,
Xenbase
Hoffmann,
Amino acid sequence microheterogeneities of basic (type II) cytokeratins of Xenopus laevis epidermis and evolutionary conservativity of helical and non-helical domains.
1985,
Pubmed
,
Xenbase
Kimelman,
Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
1987,
Pubmed
,
Xenbase
Kleinman,
Basement membrane complexes with biological activity.
1986,
Pubmed
Koenig,
Thyroid hormone receptor binds to a site in the rat growth hormone promoter required for induction by thyroid hormone.
1987,
Pubmed
Lyman,
Molecular cloning of hepatic mRNAs in Rana catesbeiana responsive to thyroid hormone during induced and spontaneous metamorphosis.
1987,
Pubmed
Mathisen,
Thyroid hormone induction of keratin genes: a two-step activation of gene expression during development.
1987,
Pubmed
,
Xenbase
McKearin,
Persistent estrogen induction of hepatic Xenopus laevis serum retinol binding protein mRNA.
1988,
Pubmed
,
Xenbase
Morris,
Thyroxine elicits divergent changes in mRNA levels for two urea cycle enzymes and one gluconeogenic enzyme in tadpole liver.
1987,
Pubmed
Nyborg,
Relationship between thyroid and glucocorticoid hormone receptor occupancy, growth hormone gene transcription, and mRNA accumulation.
1984,
Pubmed
Pinney,
Myogenic lineage determination and differentiation: evidence for a regulatory gene pathway.
1988,
Pubmed
Reeves,
Hormonal regulation of epidermis-specific protein and messenger RNA synthesis in amphibian metamorphosis.
1977,
Pubmed
,
Xenbase
Reeves,
In vitro differentiation of a homogeneous cell population--the epidermis of Xenopus laevis.
1975,
Pubmed
,
Xenbase
Sap,
The c-erb-A protein is a high-affinity receptor for thyroid hormone.
,
Pubmed
Simon,
Enzymatic cross-linking of involucrin and other proteins by keratinocyte particulates in vitro.
1985,
Pubmed
Slack,
Mesoderm induction in early Xenopus embryos by heparin-binding growth factors.
,
Pubmed
,
Xenbase
Tata,
The formation, distribution and function of ribosomes and microsomal membranes during induced amphibian metamorphosis.
1967,
Pubmed
Tata,
Early metamorphic competence of Xenopus larvae.
1968,
Pubmed
,
Xenbase
Weeks,
A maternal mRNA localized to the vegetal hemisphere in Xenopus eggs codes for a growth factor related to TGF-beta.
1987,
Pubmed
,
Xenbase
Weinberger,
The c-erb-A gene encodes a thyroid hormone receptor.
,
Pubmed
Wight,
Discrete positive and negative thyroid hormone-responsive transcription regulatory elements of the rat growth hormone gene.
1987,
Pubmed
Yisraeli,
Muscle-specific activation of a methylated chimeric actin gene.
1986,
Pubmed
