Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Anti-OTF-1 antibodies inhibit NFIII stimulation of in vitro adenovirus DNA replication.
Pruijn JM
,
van der Vliet PC
,
Dathan NA
,
Mattaj IW
.
???displayArticle.abstract???
HeLa cell OTF-1 has been purified on the basis of its DNA binding activity and used to raise a polyclonal rabbit antiserum. This antiserum is shown to recognize both native and denatured OTF-1 from both human and a similar protein from Xenopus culture cells, but to react either more weakly or not at all with the lymphoid cell-specific OTF-2. Separately, NFIII has been purified on the basis of its ability to stimulate Adenovirus DNA replication in vitro. On denaturing polyacrylamide gels OTF-1 and NFIII exhibit identical mobility. Anti-OTF-1 antiserum recognizes NFIII and neutralizes its stimulatory effect on DNA replication. Moreover, OTF-1 can functionally replace NFIII. Taken together with previously published DNA binding data, this indicates that OTF-1 and NFIII are either very closely related or identical.
Ares,
Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
1985, Pubmed,
Xenbase
Ares,
Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
1985,
Pubmed
,
Xenbase
Augereau,
The mouse immunoglobulin heavy-chain enhancer: effect on transcription in vitro and binding of proteins present in HeLa and lymphoid B cell extracts.
1986,
Pubmed
Bohmann,
A transcription factor which binds to the enhancers of SV40, immunoglobulin heavy chain and U2 snRNA genes.
,
Pubmed
,
Xenbase
Carbon,
A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II.
1987,
Pubmed
,
Xenbase
Chodosh,
Human CCAAT-binding proteins have heterologous subunits.
1988,
Pubmed
Ciliberto,
Transcription signals in embryonic Xenopus laevis U1 RNA genes.
1985,
Pubmed
,
Xenbase
Davidson,
Cell-type specific protein binding to the enhancer of simian virus 40 in nuclear extracts.
,
Pubmed
DePamphilis,
Transcriptional elements as components of eukaryotic origins of DNA replication.
1988,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Falkner,
Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements.
,
Pubmed
Fletcher,
Purification and characterization of OTF-1, a transcription factor regulating cell cycle expression of a human histone H2b gene.
1987,
Pubmed
Fried,
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
1981,
Pubmed
Galas,
DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
1978,
Pubmed
Garner,
A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.
1981,
Pubmed
Hager,
Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.
1980,
Pubmed
Harvey,
Independently evolving chicken histone H2B genes: identification of a ubiquitous H2B-specific 5' element.
1982,
Pubmed
,
Xenbase
Holst,
Murine genomic DNA sequences replicating autonomously in mouse L cells.
1988,
Pubmed
Jones,
A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.
1987,
Pubmed
Kadonaga,
Affinity purification of sequence-specific DNA binding proteins.
1986,
Pubmed
Ko,
A human protein specific for the immunoglobulin octamer DNA motif contains a functional homeobox domain.
1988,
Pubmed
Kornberg,
DNA replication.
1988,
Pubmed
Krol,
The two embryonic U1 RNA genes of Xenopus laevis have both common and gene-specific transcription signals.
1985,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Landolfi,
Interaction of cell-type-specific nuclear proteins with immunoglobulin VH promoter region sequences.
,
Pubmed
Mattaj,
An enhancer-like sequence within the Xenopus U2 gene promoter facilitates the formation of stable transcription complexes.
,
Pubmed
,
Xenbase
Mattaj,
Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins.
1985,
Pubmed
,
Xenbase
McKay,
Binding of a simian virus 40 T antigen-related protein to DNA.
1981,
Pubmed
Meisterernst,
A quantitative analysis of nuclear factor I/DNA interactions.
1988,
Pubmed
Minowada,
Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes.
1972,
Pubmed
Mocikat,
Upstream regulatory sequences of immunoglobulin genes are recognized by nuclear proteins which also bind to other gene regions.
1986,
Pubmed
,
Xenbase
Mocikat,
An ACCC-containing protein-binding sequence in the neighbourhood of the decanucleotide recognition site of the immunoglobulin gene promoter.
1988,
Pubmed
Mosthaf,
A viral enhancer element specifically active in human haematopoietic cells.
,
Pubmed
Müller,
A cloned octamer transcription factor stimulates transcription from lymphoid-specific promoters in non-B cells.
1988,
Pubmed
Ondek,
Discrete elements within the SV40 enhancer region display different cell-specific enhancer activities.
1987,
Pubmed
O'Neill,
Purification and characterization of nuclear factor III (origin recognition protein C), a sequence-specific DNA binding protein required for efficient initiation of adenovirus DNA replication.
1988,
Pubmed
O'Neill,
Transcription factor OTF-1 is functionally identical to the DNA replication factor NF-III.
1988,
Pubmed
Parslow,
Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.
1984,
Pubmed
Parslow,
The immunoglobulin octanucleotide: independent activity and selective interaction with enhancers.
1987,
Pubmed
Pruijn,
Interaction between the octamer-binding protein nuclear factor III and the adenovirus origin of DNA replication.
1988,
Pubmed
Pruijn,
Promoter and enhancer elements containing a conserved sequence motif are recognized by nuclear factor III, a protein stimulating adenovirus DNA replication.
1987,
Pubmed
Pruijn,
Nuclear factor III, a novel sequence-specific DNA-binding protein from HeLa cells stimulating adenovirus DNA replication.
,
Pubmed
,
Xenbase
Pruijn,
Inhibition of adenovirus DNA replication in vitro by autoimmune sera.
1986,
Pubmed
Rosales,
In vitro binding of cell-specific and ubiquitous nuclear proteins to the octamer motif of the SV40 enhancer and related motifs present in other promoters and enhancers.
1987,
Pubmed
Rosenfeld,
Purification of nuclear factor I by DNA recognition site affinity chromatography.
1986,
Pubmed
Rosenfeld,
Sequence-specific interactions between cellular DNA-binding proteins and the adenovirus origin of DNA replication.
1987,
Pubmed
Santoro,
A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs.
1988,
Pubmed
Scheidereit,
Identification and purification of a human lymphoid-specific octamer-binding protein (OTF-2) that activates transcription of an immunoglobulin promoter in vitro.
1987,
Pubmed
Scheidereit,
A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein.
1988,
Pubmed
Schirm,
The SV40 enhancer can be dissected into multiple segments, each with a different cell type specificity.
1987,
Pubmed
Schlokat,
Nuclear factors binding specific sequences within the immunoglobulin enhancer interact differentially with other enhancer elements.
1986,
Pubmed
Sigel,
Production of antibodies by inoculation into lymph nodes.
1983,
Pubmed
Singh,
A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
,
Pubmed
Sive,
Multiple sequence elements are required for maximal in vitro transcription of a human histone H2B gene.
1986,
Pubmed
Sive,
Interaction of a common factor with conserved promoter and enhancer sequences in histone H2B, immunoglobulin, and U2 small nuclear RNA (snRNA) genes.
1986,
Pubmed
Staudt,
A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes.
,
Pubmed
Stunnenberg,
High expression of functional adenovirus DNA polymerase and precursor terminal protein using recombinant vaccinia virus.
1988,
Pubmed
Sturm,
The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
1988,
Pubmed
Sturm,
A 100-kD HeLa cell octamer binding protein (OBP100) interacts differently with two separate octamer-related sequences within the SV40 enhancer.
1987,
Pubmed
Tebb,
Only two of the four sites of interaction with nuclear factors within the Xenopus U2 gene promoter are necessary for efficient transcription.
1987,
Pubmed
,
Xenbase
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Wildeman,
Stimulation of in vitro transcription from the SV40 early promoter by the enhancer involves a specific trans-acting factor.
1984,
Pubmed
Wirth,
An octamer oligonucleotide upstream of a TATA motif is sufficient for lymphoid-specific promoter activity.
,
Pubmed