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Conservation of structural and functional domains in complement component C3 of Xenopus and mammals.
Grossberger D
,
Marcuz A
,
Du Pasquier L
,
Lambris JD
.
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The cDNA sequence and the deduced amino acid sequence of the Mr 34,000 C-terminal fragment of Xenopus laevis complement component C3 are presented. The sequence of Xenopus C3 has 57% nucleotide identity to the corresponding sequence of human C3 and approximately 49% amino acid identity to C3 from human, mouse, and rabbit. The Xenopus C3 sequence shows clusters of high and of low similarity to the mammalian C3 sequences. One of these regions of high similarity represents the domain of mammalian C3b involved in the binding of properdin, a regulator of the alternative pathway of complement activation. It is not clear whether the other highly conserved regions are involved in binding to other C3 ligands. The Xenopus C3 sequence completely lacks the Arg-Gly-Asp sequence, which has been suggested to be the recognition site of the human complement receptor type 3 on the iC3b fragment of human C3. The Xenopus C3 gene is shown not to be linked to the Xenopus major histocompatibility complex, as is also the case in mammals. Since the gene of the related molecule C4 is MHC-linked in both mammals and Xenopus, the C3 and C4 genes may have separated before Xenopus and mammals speciated.
Alper,
Human C'3: evidence for the liver as the primary site of synthesis.
1969, Pubmed
Alper,
Human C'3: evidence for the liver as the primary site of synthesis.
1969,
Pubmed
Belt,
The structural basis of the multiple forms of human complement component C4.
1984,
Pubmed
Chen,
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
1985,
Pubmed
Daoudaki,
A 34-amino acid peptide of the third component of complement mediates properdin binding.
1988,
Pubmed
de Bruijn,
Human complement component C3: cDNA coding sequence and derived primary structure.
1985,
Pubmed
Eggertsen,
Antigenic relationships between human and cobra complement factors C3 and cobra venom factor (CVF) from the Indian cobra (Naja naja).
1983,
Pubmed
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Ferreira,
Genetic linkage between serum levels of the third component of complement and the H-2 complex.
1975,
Pubmed
Fujii,
Characterization of the fourth component of complement in the serum of the clawed frog Xenopus laevis.
1985,
Pubmed
,
Xenbase
Jurd,
A natural heterohaemagglutinin in Xenopus laevis serum.
1978,
Pubmed
,
Xenbase
Kai,
Isolation and identification of the third component of complement of Japanese quails.
1983,
Pubmed
Kaidoh,
Phylogeny of C4b-C3b cleaving activity: similar fragmentation patterns of human C4b and C3b produced by lower animals.
1987,
Pubmed
Kan,
Nucleotide sequence of cDNA encoding human alpha 2-macroglobulin and assignment of the chromosomal locus.
1985,
Pubmed
Klein,
Evolution of the major histocompatibility complex.
1986,
Pubmed
Kusano,
Nucleotide sequence of cDNA and derived amino acid sequence of rabbit complement component C3 alpha-chain.
1986,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lambris,
The multifunctional role of C3: structural analysis of its interactions with physiological ligands.
1986,
Pubmed
Lambris,
The multifunctional role of C3, the third component of complement.
1988,
Pubmed
Nakamura,
Cosegregation of the polymorphic C4 with the MHC in the frog, Xenopus laevis.
1986,
Pubmed
,
Xenbase
Needleman,
A general method applicable to the search for similarities in the amino acid sequence of two proteins.
1970,
Pubmed
Nonaka,
Purification of a lamprey complement protein homologous to the third component of the mammalian complement system.
1984,
Pubmed
Nonaka,
Purification of a major serum protein of rainbow trout (Salmo gairdneri) homologous to the third component of mammalian complement.
1984,
Pubmed
Pangburn,
The alternative pathway of complement.
1984,
Pubmed
Ruoslahti,
Arg-Gly-Asp: a versatile cell recognition signal.
1986,
Pubmed
Schwager,
Organization and rearrangement of immunoglobulin M genes in the amphibian Xenopus.
1988,
Pubmed
,
Xenbase
Sekizawa,
Isolation and characterization of the third component of complement in the serum of the clawed frog, Xenopus laevis.
1984,
Pubmed
,
Xenbase
Sekizawa,
Membrane receptors on Xenopus macrophages for two classes of immunoglobulins (IgM and IgY) and the third complement component (C3).
1984,
Pubmed
,
Xenbase
Simmons,
ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM.
1988,
Pubmed
Sottrup-Jensen,
Common evolutionary origin of alpha 2-macroglobulin and complement components C3 and C4.
1985,
Pubmed
Tack,
The beta-Cys-gamma-Glu thiolester bond in human C3, C4, and alpha 2-macroglobulin.
1983,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Ullrich,
Rat insulin genes: construction of plasmids containing the coding sequences.
1977,
Pubmed
Vogelstein,
Preparative and analytical purification of DNA from agarose.
1979,
Pubmed
Wetsel,
Primary structure of the fifth component of murine complement.
1987,
Pubmed
Wetsel,
Structure of murine complement component C3. II. Nucleotide sequence of cloned complementary DNA coding for the alpha chain.
1984,
Pubmed
Whitehead,
Assignment of the structural gene for the third component of human complement to chromosome 19.
1982,
Pubmed
Wright,
C3bi receptor (complement receptor type 3) recognizes a region of complement protein C3 containing the sequence Arg-Gly-Asp.
1987,
Pubmed
Young,
Efficient isolation of genes by using antibody probes.
1983,
Pubmed
Young,
Yeast RNA polymerase II genes: isolation with antibody probes.
1983,
Pubmed
