Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Enzymatic amplification of translation inhibition of rabbit beta-globin mRNA mediated by anti-messenger oligodeoxynucleotides covalently linked to intercalating agents.
Cazenave C
,
Loreau N
,
Thuong NT
,
Toulmé JJ
,
Hélène C
.
???displayArticle.abstract???
The effects of anti-messenger oligodeoxynucleotides, covalently linked to an intercalating agent, on translation of rabbit beta-globin mRNA, were investigated both in wheat germ extract and in microinjected Xenopus oocytes. A specific inhibition of beta-globin synthesis was observed in both expression systems with a modified 11-mer covalently linked to an acridine derivative. In injected oocytes a more efficient block was observed with this modified oligonucleotide than with its unsubstituted homolog. This was ascribed to stacking interactions of the intercalating agent with base pairs which provide an additional stabilization of the [mRNA/DNA] hybrid. We demonstrated that in wheat germ extract, the modified and unmodified oligonucleotides behaved similarly due to the presence of a high RNaseH activity. RNaseH was also present, although to a lesser extent, in the oocytecytoplasm. This anti-messenger DNA-induced degradation of target mRNA resulted in amplified efficiency of hybrid-arrested translation. This additional mechanism might provide anti-sense DNAs with an advantage over anti-sense RNAs.
Agris,
Inhibition of vesicular stomatitis virus protein synthesis and infection by sequence-specific oligodeoxyribonucleoside methylphosphonates.
1986, Pubmed
Agris,
Inhibition of vesicular stomatitis virus protein synthesis and infection by sequence-specific oligodeoxyribonucleoside methylphosphonates.
1986,
Pubmed
Amini,
Regulation of pp60c-src synthesis by inducible RNA complementary to c-src mRNA in polyomavirus-transformed rat cells.
1986,
Pubmed
Asseline,
Oligodeoxynucleotides covalently linked to intercalating dyes as base sequence-specific ligands. Influence of dye attachment site.
1984,
Pubmed
Asseline,
[New substances with high specific activity for nucleic acid sequences: oligodeoxynucleotides covalently linked to an intercalating agent].
1983,
Pubmed
Auron,
Computer-aided prediction of RNA secondary structures.
1982,
Pubmed
Blake,
Inhibition of rabbit globin mRNA translation by sequence-specific oligodeoxyribonucleotides.
1985,
Pubmed
Blake,
Hybridization arrest of globin synthesis in rabbit reticulocyte lysates and cells by oligodeoxyribonucleoside methylphosphonates.
1985,
Pubmed
Cathala,
Isolation and characterization of two types of ribonucleases H in Krebs II ascites cells.
1979,
Pubmed
Cazenave,
Anti-messenger oligodeoxynucleotides: specific inhibition of rabbit beta-globin synthesis in wheat germ extracts and Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Chen,
Nuclease activity of 1,10-phenanthroline-copper: sequence-specific targeting.
1986,
Pubmed
Chu,
Nonenzymatic sequence-specific cleavage of single-stranded DNA.
1985,
Pubmed
Coleman,
The use of RNAs complementary to specific mRNAs to regulate the expression of individual bacterial genes.
1984,
Pubmed
Cornelissen,
The common 5' terminal sequence on trypanosome mRNAs: a target for anti-messenger oligodeoxynucleotides.
1986,
Pubmed
Crowley,
Phenocopy of discoidin I-minus mutants by antisense transformation in Dictyostelium.
1985,
Pubmed
Dreyer,
Sequence-specific cleavage of single-stranded DNA: oligodeoxynucleotide-EDTA X Fe(II).
1985,
Pubmed
Ecker,
Inhibition of gene expression in plant cells by expression of antisense RNA.
1986,
Pubmed
Efstratiadis,
The primary structure of rabbit beta-globin mRNA as determined from cloned DNA.
1977,
Pubmed
Giglioni,
Translational control of globin synthesis by haemin in Xenopus oocytes.
1973,
Pubmed
,
Xenbase
Green,
The role of antisense RNA in gene regulation.
1986,
Pubmed
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Haberkern,
Studies on a calf thymus ribonuclease specific for ribonucleic acid-deoxyribonucleic acid hybrids.
1973,
Pubmed
Haeuptle,
Translation arrest by oligodeoxynucleotides complementary to mRNA coding sequences yields polypeptides of predetermined length.
1986,
Pubmed
Harland,
Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA.
1985,
Pubmed
,
Xenbase
Hastie,
Analysis of mRNA populations by cDNA.mRNA hybrid-mediated inhibition of cell-free protein synthesis.
1978,
Pubmed
Hillenbrand,
Discriminatory function of ribonuclease H in the selective initiation of plasmid DNA replication.
1982,
Pubmed
Hirashima,
Engineering of the mRNA-interfering complementary RNA immune system against viral infection.
1986,
Pubmed
Holt,
Inducible production of c-fos antisense RNA inhibits 3T3 cell proliferation.
1986,
Pubmed
Huez,
Degradation of deadenylated rabbit alpha-globin mRNA in Xenopus oocytes is associated with its translation.
1977,
Pubmed
,
Xenbase
Izant,
Inhibition of thymidine kinase gene expression by anti-sense RNA: a molecular approach to genetic analysis.
1984,
Pubmed
Izant,
Constitutive and conditional suppression of exogenous and endogenous genes by anti-sense RNA.
1985,
Pubmed
,
Xenbase
Kawasaki,
Quantitative hybridization-arrest of mRNA in Xenopus oocytes using single-stranded complementary DNA or oligonucleotide probes.
1985,
Pubmed
,
Xenbase
Kim,
Stable reduction of thymidine kinase activity in cells expressing high levels of anti-sense RNA.
1985,
Pubmed
Lancelot,
Proton and phosphorus nuclear magnetic resonance studies of an oligothymidylate covalently linked to an acridine derivative and of its binding to complementary sequences.
1985,
Pubmed
Le Doan,
Targeted cleavage of polynucleotides by complementary oligonucleotides covalently linked to iron-porphyrins.
1986,
Pubmed
Liebhaber,
Translationally associated helix-destabilizing activity in rabbit reticulocyte lysate.
1984,
Pubmed
Light,
Post-transcriptional control of expression of the repA gene of plasmid R1 mediated by a small RNA molecule.
1983,
Pubmed
McGarry,
Inhibition of heat shock protein synthesis by heat-inducible antisense RNA.
1986,
Pubmed
Melton,
Injected anti-sense RNAs specifically block messenger RNA translation in vivo.
1985,
Pubmed
,
Xenbase
Miller,
Control of ribonucleic acid function by oligonucleoside methylphosphonates.
1985,
Pubmed
Minshull,
The use of single-stranded DNA and RNase H to promote quantitative 'hybrid arrest of translation' of mRNA/DNA hybrids in reticulocyte lysate cell-free translations.
1986,
Pubmed
Mizuno,
A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA).
1984,
Pubmed
Nepveu,
Intragenic pausing and anti-sense transcription within the murine c-myc locus.
1986,
Pubmed
Okamoto,
Mechanism for the autogenous control of the crp operon: transcriptional inhibition by a divergent RNA transcript.
1986,
Pubmed
Paterson,
Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.
1977,
Pubmed
Rosenberg,
Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos.
,
Pubmed
Rovera,
Resolution of hemoglobin subunits by electrophoresis in acid urea polyacrylamide gels containing Triton X-100.
1978,
Pubmed
Rubenstein,
[Nonsense RNA: a tool for specifically inhibiting the expression of a gene in vivo].
1984,
Pubmed
Shakin,
Destabilization of messenger RNA/complementary DNA duplexes by the elongating 80 S ribosome.
1986,
Pubmed
Simons,
Translational control of IS10 transposition.
1983,
Pubmed
Smith,
Antiviral effect of an oligo(nucleoside methylphosphonate) complementary to the splice junction of herpes simplex virus type 1 immediate early pre-mRNAs 4 and 5.
1986,
Pubmed
Spencer,
Overlapping transcription units in the dopa decarboxylase region of Drosophila.
,
Pubmed
Stephenson,
Inhibition of Rous sarcoma viral RNA translation by a specific oligodeoxyribonucleotide.
1978,
Pubmed
Toulmé,
Specific inhibition of mRNA translation by complementary oligonucleotides covalently linked to intercalating agents.
1986,
Pubmed
Walder,
The 35-nucleotide spliced leader sequence is common to all trypanosome messenger RNA's.
1986,
Pubmed
Williams,
A mouse locus at which transcription from both DNA strands produces mRNAs complementary at their 3' ends.
,
Pubmed
Zamecnik,
Inhibition of Rous sarcoma virus replication and cell transformation by a specific oligodeoxynucleotide.
1978,
Pubmed
Zamecnik,
Inhibition of replication and expression of human T-cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotides complementary to viral RNA.
1986,
Pubmed
