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We have recently shown that extensive proliferation of liver parenchymal cells takes place in adult male Xenopus frogs in response to estradiol-17 beta, which also induces synthesis and secretion of vitellogenin, the precursor of yolk proteins. We demonstrate here that liver parenchymal cells from adult male animals can be maintained for several weeks in a defined tissue culture medium containing added insulin, dexamethasone, and triiodothyronine. Under these conditions the cells do not divide, but can synthesize DNA. Maximum DNA synthesis occurs in cells that have achieved monolayer morphology under low plating densities. Estradiol-17 beta causes a dose-dependent increase in the number of cells synthesizing DNA, as well as inducing synthesis of vitellogenin. Estrogen-dependent, but not background, DNA synthesis is inhibited by the antiestrogen tamoxifen. These results imply that estradiol-17 beta acts directly on liver cells to initiate DNA replication, probably by interaction with a receptor protein and induction of specific gene transcription.
Brock,
Estrogen regulates the absolute rate of transcription of the Xenopus laevis vitellogenin genes.
1983, Pubmed,
Xenbase
Brock,
Estrogen regulates the absolute rate of transcription of the Xenopus laevis vitellogenin genes.
1983,
Pubmed
,
Xenbase
Bucher,
Regulation of hepatic regeneration in rats by synergistic action of insulin and glucagon.
1975,
Pubmed
Chalbos,
Estrogens stimulate cell proliferation and induce secretory proteins in a human breast cancer cell line (T47D).
1982,
Pubmed
Green,
Direct induction by estradiol on vitellogenin synthesis in organ cultures of male Xenopus laevis liver.
1976,
Pubmed
,
Xenbase
Gschwendt,
Inhibition of the estrogen-induced synthesis of vitellogenin mRNA in chick liver by tamoxifen.
1983,
Pubmed
Hasegawa,
Induction of mitosis in primary cultures of adult rat hepatocytes under serum-free conditions.
1982,
Pubmed
Hayward,
Coordinate estrogen induction of vitellogenin and a small serum protein mRNA in Xenopus laevis liver.
1985,
Pubmed
,
Xenbase
Holland,
Estrogen induction of a 45 kDa secreted protein coordinately with vitellogenin in Xenopus liver.
1987,
Pubmed
,
Xenbase
Horwitz,
Nuclear mechanisms of estrogen action. Effects of estradiol and anti-estrogens on estrogen receptors and nuclear receptor processing.
1978,
Pubmed
Johnson-Wint,
A rapid in situ deoxyribonucleic acid assay for determining cell number in culture and tissue.
1982,
Pubmed
Kaye,
Kinetics of DNA synthesis in immature rat uterus: age dependence and estradiol stimulation.
1971,
Pubmed
Laishes,
Conditions affecting primary cell cultures of functional adult rat hepatocytes. II. Dexamethasone enhanced longevity and maintenance of morphology.
1976,
Pubmed
May,
Estrogen dramatically decreases albumin mRNA levels and albumin synthesis in Xenopus laevis liver.
1982,
Pubmed
,
Xenbase
McGowan,
Pyruvate promotion of DNA synthesis in serum-free primary cultures of adult rat hepatocytes.
1983,
Pubmed
Morley,
Use of 3 H-thymidine for measurement of DNA synthesis in rat liver--a warning.
1972,
Pubmed
Morley,
Stimulation of hepatocellular proliferation by a serum factor from thioacetamide-treated rats.
1977,
Pubmed
Nakamura,
Loss of reciprocal modulations of growth and liver function of hepatoma cells in culture by contact with cells or cell membranes.
1984,
Pubmed
Nakamura,
Reciprocal modulation of growth and differentiated functions of mature rat hepatocytes in primary culture by cell--cell contact and cell membranes.
1983,
Pubmed
Oka,
Interaction of estrogen and progesterone in chick oviduct development. I. Antagonistic effect of progesterone on estrogen-induced proliferation and differentiation of tubular gland cells.
1969,
Pubmed
Palmiter,
Interaction of estrogen and progesterone in chick oviduct development. 3. Tubular gland cell cytodifferentiation.
1971,
Pubmed
Richman,
Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes.
1976,
Pubmed
Seaver,
The chick oviduct in tissue culture. I. Initial characterization of growing primary oviduct tissue cultures.
1984,
Pubmed
Shyamala,
The nonresponsiveness of lactating mammary gland to estradiol.
1982,
Pubmed
Soto,
Mechanism of estrogen action on cellular proliferation: evidence for indirect and negative control on cloned breast tumor cells.
1984,
Pubmed
Spolski,
Estrogen-dependent DNA synthesis and parenchymal cell proliferation in the liver of adult male Xenopus frogs.
1985,
Pubmed
,
Xenbase
Tomomura,
Role of the cytoskeleton in glycogenolysis stimulated by glucagon in primary cultures of adult rat hepatocytes.
1980,
Pubmed
Topper,
Multiple hormone interactions in the developmental biology of the mammary gland.
1980,
Pubmed
Wahli,
Vitellogenesis and the vitellogenin gene family.
1981,
Pubmed
,
Xenbase
Wangh,
Thyroid hormones are corequisites for estradiol-17beta in vitro induction of Xenopus vitellogenin synthesis and secretion.
1982,
Pubmed
,
Xenbase
Wangh,
Glucocorticoids act together with estrogens and thyroid hormones in regulating the synthesis and secretion of Xenopus vitellogenin, serum albumin, and fibrinogen.
1982,
Pubmed
,
Xenbase
Wangh,
Xenopus fibrinogen. Characterization of subunits and hormonal regulation of biosynthesis.
1983,
Pubmed
,
Xenbase
Wangh,
Parenchymal cells purified from Xenopus liver and maintained in primary culture synthesize vitellogenin in response to estradiol-17 beta and serum albumin in response to dexamethasone.
1979,
Pubmed
,
Xenbase
Wangh,
Synthesis of vitellogenin in cultures of male and female frog liver regulated by estradiol treatment in vitro.
1975,
Pubmed
,
Xenbase
Westley,
An estrogen receptor from Xenopus laevis liver possibly connected with vitellogenin synthesis.
1978,
Pubmed
,
Xenbase
Wiklund,
Genetic differences in estrogen-induced deoxyribonucleic acid synthesis in the rat pituitary: correlations with pituitary tumor susceptibility.
1982,
Pubmed
