Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
DNA (from bacteriophage lambda or Xenopus) is assembled into nucleus-like structures when mixed with an extract from Xenopus eggs. Electron microscopy shows that these in vitro-reconstituted nuclei possess complete double membranes; some, but not all, nuclei have pore complexes. Extracts depleted of their endogenous ATP (by addition of ATPases) cannot assemble nuclear envelopes visible by phase-contrast microscopy. Once synthetic nuclei are assembled, however, they are stable when ATP is subsequently depleted, although their chromatin becomes condensed. About one-fourth of the nuclei assembled in vitro from lambda DNA accumulate nuclear proteins such as nucleoplasmin. ATP depletion blocks nucleoplasmin accumulation both in vitro, in pre-assembled synthetic nuclei, and in vivo, in the nucleus of microinjected oocytes. However, nucleoplasmin previously accumulated by reconstituted nuclei or by the germinal vesicle in microinjected oocytes is retained after ATP depletion.
Barry,
Swelling of hen erythrocyte nuclei in cytoplasm from Xenopus eggs.
1972, Pubmed,
Xenbase
Barry,
Swelling of hen erythrocyte nuclei in cytoplasm from Xenopus eggs.
1972,
Pubmed
,
Xenbase
Berrios,
Characterization of an ATPase/dATPase activity associated with the Drosophila nuclear matrix-pore complex-lamina fraction. Identification of the putative enzyme polypeptide by direct ultraviolet photoaffinity labeling.
1983,
Pubmed
Bonner,
Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins.
1975,
Pubmed
,
Xenbase
Cande,
Nucleotide requirements for anaphase chromosome movements in permeabilized mitotic cells: anaphase B but not anaphase A requires ATP.
1982,
Pubmed
Chardonnet,
Early events in the interaction of adenoviruses with HeLa cells. 3. Relationship between an ATPase activity in nuclear envelopes and transfer of core material: a hypothesis.
1972,
Pubmed
Dabauvalle,
Karyophilic proteins: polypeptides synthesized in vitro accumulate in the nucleus on microinjection into the cytoplasm of amphibian oocytes.
1982,
Pubmed
,
Xenbase
Davey,
Identification of the sequence responsible for the nuclear accumulation of the influenza virus nucleoprotein in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
De Robertis,
Intracellular migration of nuclear proteins in Xenopus oocytes.
1978,
Pubmed
,
Xenbase
De Robertis,
Nucleocytoplasmic segregation of proteins and RNAs.
1983,
Pubmed
Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase
Earnshaw,
Assembly of nucleosomes: the reaction involving X. laevis nucleoplasmin.
1980,
Pubmed
,
Xenbase
Feldherr,
Evidence for mediated protein uptake by amphibian oocyte nuclei.
1983,
Pubmed
Feldherr,
Mechanism for the selection of nuclear polypeptides in Xenopus oocytes. II. Two-dimensional gel analysis.
1980,
Pubmed
,
Xenbase
Feldherr,
Movement of a karyophilic protein through the nuclear pores of oocytes.
1984,
Pubmed
,
Xenbase
Foe,
Reversible chromosome condensation induced in Drosophila embryos by anoxia: visualization of interphase nuclear organization.
1985,
Pubmed
Forbes,
Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs.
1983,
Pubmed
,
Xenbase
Gerace,
The nuclear envelope lamina is reversibly depolymerized during mitosis.
1980,
Pubmed
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Gurdon,
Nuclear transplantation and the control of gene activity in animal development.
1970,
Pubmed
Kleinschmidt,
Co-existence of two different types of soluble histone complexes in nuclei of Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase
Krohne,
Immunological identification and localization of the predominant nuclear protein of the amphibian oocyte nucleus.
1980,
Pubmed
,
Xenbase
Lohka,
Roles of cytosol and cytoplasmic particles in nuclear envelope assembly and sperm pronuclear formation in cell-free preparations from amphibian eggs.
1984,
Pubmed
,
Xenbase
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Miake-Lye,
Induction of early mitotic events in a cell-free system.
1985,
Pubmed
,
Xenbase
Mills,
An acidic protein which assembles nucleosomes in vitro is the most abundant protein in Xenopus oocyte nuclei.
1980,
Pubmed
,
Xenbase
Ottaviano,
Phosphorylation of the nuclear lamins during interphase and mitosis.
1985,
Pubmed
Scheer,
Negative staining and adenosine triphosphatase activity of annulate lamellae of newt oocytes.
1969,
Pubmed
Stick,
Changes in the nuclear lamina composition during early development of Xenopus laevis.
1985,
Pubmed
,
Xenbase
Yasuzumi,
The fine structure of nuclei as revealed by electron microscopy. 3. Adenosine triphosphatase activity in the pores of nuclear envelope of mouse choroid plexus epithelial cells.
1966,
Pubmed
