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XB-ART-2897
FEBS Lett 2004 Oct 08;5761-2:63-7. doi: 10.1016/j.febslet.2004.08.060.
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Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2.

Omae F , Miyazaki M , Enomoto A , Suzuki A .


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Although the amino acid sequences of mouse DES1 (MDES1) and DES2 (MDES2) have 63% sequence identity, their enzymatic characteristics are quite different. MDES1 exhibits high dihydroceramide delta4-desaturase activity and very low C-4 hydroxylase activity, while MDES2 is similarly active as both a dihydroceramide delta4-desaturase and a C-4 hydroxylase. We constructed several chimeras of MDES1 and MDES2 and identified a region important for C-4 hydroxylase activity in MDES2. This region contains the sequence XAFGY (X=T or A or V; Y=T or N) and occurs on the C-terminal side of the first His-box of MDES2. We confirmed the conservation of this region in DES2 family members sequenced from humans, pigs, rats, chickens, zebrafish, and Xenopus.

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Species referenced: Xenopus
Genes referenced: degs1 degs2