Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Differential sensitization to deoxyribonuclease I of Xenopus vitellogenin and albumin genes during primary and secondary induction of vitellogenesis by oestradiol.
???displayArticle.abstract???
The sensitivity to DNAase (deoxyribonuclease) I (which preferentially digests transcribed sequences) of vitellogenin and albumin genes in liver and erythrocytes of male Xenopus after primary and secondary induction of vitellogenesis by oestrogen was measured by hybridization to cDNA (complementary DNA) of the residual DNA after enzymic digestion of isolated nuclei. Vitellogenin sequences were rendered selectively more sensitive to limited DNAase-I digestion (15-20% of DNA rendered acid-soluble) during primary hormonal activation (5 days) of vitellogenin genes in liver, but not erythrocyte, nuclei. Hormone withdrawal (25 days after first injection) did not result in reversion to a pre-activation gene configuration, nor did secondary hormonal stimulation (5 days after second and 25 days after first injection) augment the sensitivity of the genes to digestion by the nuclease. Similar hormone treatment did not affect the sensitivity of the constitutively expressed albumin genes in liver nuclei, nor their insensitivity in erythrocyte nuclei. Under the same conditions, globin genes remained indigestible in liver nuclei. It is concluded that primary induction of vitellogenesis in male Xenopus liver is accompanied by relatively long-lasting (3-4 weeks) change in the configuration of vitellogenin genes in hepatic nuclei which is not reversed or further modified during short-term oestrogen withdrawal or upon secondary stimulation.
Baker,
Kinetics of estrogen induction of Xenopus laevis vitellogenin messenger RNA as measured by hybridization to complementary DNA.
1977, Pubmed,
Xenbase
Baker,
Kinetics of estrogen induction of Xenopus laevis vitellogenin messenger RNA as measured by hybridization to complementary DNA.
1977,
Pubmed
,
Xenbase
Bellard,
Nucleosome structure III: the structure and transcriptional activity of the chromatin containing the ovalbumin and globin genes in chick oviduct nuclei.
1978,
Pubmed
Britten,
Analysis of repeating DNA sequences by reassociation.
1974,
Pubmed
BURTON,
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
1956,
Pubmed
Dawid,
Application of recombinant DNA technology to questions of developmental biology: a review.
1979,
Pubmed
Deeley,
Kinetics of avian vitellogenin messenger RNA induction. Comparison between primary and secondary response to estrogen.
1977,
Pubmed
Dimitriadis,
Isolation and characterization of xenopus laevis albumin mRNA.
1981,
Pubmed
,
Xenbase
Dimitriadis,
Subnuclear fractionation by mild micrococcal-nuclease treatment of nuclei of different transcriptional activities causes a partition of expressed and non-expressed genes.
1980,
Pubmed
,
Xenbase
Farmer,
Translation of Xenopus vitellogenin mRNA during primary and secondary induction.
1978,
Pubmed
,
Xenbase
Felber,
Isolation and translation in vitro of four related vitellogenin mRNAs of estrogen-stimulated Xenopus laevis.
1980,
Pubmed
,
Xenbase
Felsenfeld,
Chromatin.
1978,
Pubmed
Flint,
An altered subunit configuration associated with the actively transcribed DNA of integrated adenovirus genes.
1977,
Pubmed
Garel,
Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.
1976,
Pubmed
Garel,
Genes transcribed at diverse rates have a similar conformation in chromatin.
1977,
Pubmed
Gerber-Huber,
Estrogen induces tissue specific changes in the chromatin conformation of the vitellogenin genes in Xenopus.
1981,
Pubmed
,
Xenbase
Gordon,
Cloning of a double-stranded cDNA that codes for a portion of chicken preproalbumin. A general method for isolating a specific DNA sequence from partially purified mRNA.
1978,
Pubmed
Gross-Bellard,
Isolation of high-molecular-weight DNA from mammalian cells.
1973,
Pubmed
Groudine,
Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation.
1981,
Pubmed
Hayward,
Induction of estrogen receptor and reversal of the nuclear/cytoplasmic receptor ratio during vitellogenin synthesis and withdrawal in Xenopus laevis.
1980,
Pubmed
,
Xenbase
McMaster,
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
1977,
Pubmed
Miller,
Active conformation of the globin genes in uninduced and induced mouse erythroleukemia cells.
1978,
Pubmed
Noll,
Action of micrococcal nuclease on chromatin and the location of histone H1.
1977,
Pubmed
O'Brien,
Oxidation-induced lymphocyte transformation.
1976,
Pubmed
Palmiter,
Regulation of gene expression in the chick oviduct by steroid hormones.
1978,
Pubmed
Panet,
Selective degradation of integrated murine leukemia proviral DNA by deoxyribonucleases.
1977,
Pubmed
Searle,
Vitellogenin gene expression in male Xenopus hepatocytes during primary and secondary stimulation with estrogen in cell cultures.
1981,
Pubmed
,
Xenbase
Stalder,
Hb switching in chickens.
1980,
Pubmed
Stalder,
Tissue-specific DNA cleavages in the globin chromatin domain introduced by DNAase I.
1980,
Pubmed
Tata,
Vitellogenin as a multigene family. Not all Xenopus vitellogenin genes may be in an "expressible" configuration.
1980,
Pubmed
,
Xenbase
Tata,
Vitellogenesis: a versatile model for hormonal regulation of gene expression.
1979,
Pubmed
,
Xenbase
Wachsmuth,
Localization of vitellogenin and serum albumin in hepatic parenchymal cells of normal and estradiol-treated immature chickens.
1976,
Pubmed
Wahli,
Vitellogenin in Xenopus laevis is encoded in a small family of genes.
1979,
Pubmed
,
Xenbase
Wahli,
Vitellogenesis and the vitellogenin gene family.
1981,
Pubmed
,
Xenbase
Wangh,
Parenchymal cells purified from Xenopus liver and maintained in primary culture synthesize vitellogenin in response to estradiol-17 beta and serum albumin in response to dexamethasone.
1979,
Pubmed
,
Xenbase
Weintraub,
Alpha-Globin-gene switching during the development of chicken embryos: expression and chromosome structure.
1981,
Pubmed
Weintraub,
Chromosomal subunits in active genes have an altered conformation.
1976,
Pubmed
Westley,
Estrogen causes a rapid, large and prolonged rise in the level of nuclear estrogen receptor in Xenopus laevis liver.
1979,
Pubmed
,
Xenbase
